Antibody:	anti-Brn3.0
Detection system:	horseradish peroxidase
Assay notes:	Specimens were fixed in 60% ethanol and 4% formaldehyde.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	2a	Expression in the tectum.

Structure	Level	Pattern	Image	Note
TS17: trigeminal ganglion	Present		2b

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2g	Expression in dorsal cells forming a distinct layer. Weak expression in occasional cells among the differentiated ventral motoneurons.
TS17: trigeminal ganglion	Present		2g	Expression in trigeminal neural precursors.

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present	Regionally restricted	2h	Expression in a majority cells in the extensive ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS27: midbrain	Present	Non-Uniform	3a	Expression in the cells of differing size and intensity organized in distinct layers in the midbrain.
TS27: superior colliculus	Present		3a

Structure	Level	Pattern	Image	Note
TS27: nucleus ambiguus	Present		3b
TS27: red nucleus	Present		3b
TS27: superior colliculus	Present		3b

Structure	Level	Pattern	Image	Note
TS27: red nucleus	Present		3c

Structure	Level	Pattern	Image	Note
TS27: trigeminal V mesencephalic nucleus	Present		3d	Expression in large cells with the characteristic location and morphology of mesencephalic nucleus of the trigeminal (MesV) neurons.

Structure	Level	Pattern	Image	Note
TS27: inferior olivary nucleus	Present		3e
TS27: nucleus ambiguus	Present		3e
TS27: medulla oblongata	Present	Scattered	3e	Expression in numerous, scattered reticular cells in the caudal medulla.

Structure	Level	Pattern	Image	Note
TS27: spinal cord dorsal horn	Present	Regionally restricted	3f	Expression in neurons concentrated primarily in the dorsal and intermediate gray.
TS27: spinal cord gray matter	Present	Regionally restricted	3f	Expression in neurons concentrated primarily in the dorsal and intermediate gray.
TS27: spinal cord ventral horn	Absent		3f	No significant expression in ventral motor horn neurons.

Structure	Level	Pattern	Image	Note
TS17: trigeminal ganglion	Present	Regionally restricted	5f	Expression in dividing cells in the precursors of the trigeminal ganglion.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	5g	Expression in dividing cells in the precursors of the vestibulocochlear ganglion.