Gene Expression Data
Assay Details
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Assay
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Reference:
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J:29932
Fedtsova NG, et al., Brn-3.0 expression identifies early post-mitotic CNS neurons and sensory neural precursors. Mech Dev. 1995 Nov;53(3):291-304
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5468306
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Gene symbol:
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Pou4f1
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Gene name:
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POU domain, class 4, transcription factor 1
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Antibody:
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anti-Brn3.0
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Assay notes:
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Specimens were fixed in 60% ethanol and 4% formaldehyde.
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Results
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Specimen
not shown E8.5:
embryonic day 8.5
(more  )
Specimen
not shown E8.5:
(close  )
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Genetic Background:
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involves: BALB/c * C57BL/6
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Present |
Regionally restricted |
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Expression in occasional cells near midline of rostral mesencephalic tectum, prior to closure of anterior neuropore.
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Specimen
not shown E9.0:
embryonic day 9.0
(more )
Note: 20 somites.
Specimen
not shown E9.0:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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20 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future midbrain |
Present |
Regionally restricted |
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Expression in a significantly increased number of tectal neurons.
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| TS14: trigeminal neural crest |
Present |
Regionally restricted |
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Expression in migrating precursors of the trigeminal ganglion.
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Specimen
not shown E9.5:
embryonic day 9.5
(more )
Specimen
not shown E9.5:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
not shown E10.5:
embryonic day 10.5
(more )
Specimen
not shown E10.5:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
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Prominent expression at the dorsolateral margin of the caudal medulla.
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| TS17: 1st branchial arch mesenchyme |
Present |
Regionally restricted |
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Expression in a small number of cells within the mesenchyme of the 1st and 2nd branchial arches.
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| TS17: 2nd branchial arch mesenchyme |
Present |
Regionally restricted |
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Expression in a small number of cells within the mesenchyme of the 1st and 2nd branchial arches.
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Specimen
not shown E12.5:
embryonic day 12.5
(more )
Specimen
not shown E12.5:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
Present |
Regionally restricted |
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Expression in the pretectum; expressing cells adjacent to fibers of posterior commissure.
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| TS20: epithalamus |
Present |
Regionally restricted |
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Expression in the medial and lateral habenula.
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| TS20: retina |
Weak |
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Weak expression in a few cells in the retina.
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Specimen
not shown E13.5:
embryonic day 13.5
(more )
Specimen
not shown E13.5:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: neural retinal epithelium |
Present |
Regionally restricted |
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Expression in a centrally located layer of retinal ganglion cells.
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Specimen
not shown neonatal:
postnatal day 1
(more )
Specimen
not shown neonatal:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: pretectum |
Present |
Regionally restricted |
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Expression in the pretectum, including the medial pretectal nucleus, and scattered cells surrounding the habenulo-peduncular tract.
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| TS27: midbrain |
Present |
Regionally restricted |
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Expression in midbrain nuclei including the nucleus of the lateral lemniscus and midline nuclei of the oculomotor reflex system.
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Specimen
not shown postnatal:
postnatal
(more )
Specimen
not shown postnatal:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: ganglion |
Present |
Regionally restricted |
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Expression in all sensory ganglia.
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