Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:58583
Fukada K, et al., In situ hybridisation study of type I, II, X collagens and aggrecan mRNas in the developing condylar cartilage of fetal mouse mandible. J Anat. 1999 Oct;195(Pt 3):321-9
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5468299
|
Gene symbol:
|
Acan
|
Gene name:
|
aggrecan
|
Probe:
|
Aggrecan cDNA2
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Results
|
Specimen
1b & 1d:
embryonic day 14.5
(more )
Specimen
1b & 1d:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: mandible |
Present |
Regionally restricted |
1d
,
1b
|
Expression was detected adjacent to the ossifying mandibular bone anlage. Expression was also noted in the mesenchymal cell aggregation of the most posterior region.
|
Specimen
2d:
embryonic day 15.0
(more )
Specimen
2d:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: mandible |
Present |
Regionally restricted |
2d
|
Expression was detected in hypertrophic chondrocytes of the cartilage matrix.
|
Specimen
3d & 4b:
embryonic day 16.0
(more )
Specimen
3d & 4b:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 16.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: mandible |
Present |
Regionally restricted |
4b
,
3d
|
Expression was detected from the fibrous cell layer to the bottom of the hypertrophic layer in the condylar cartilage of the mandible. This includes the polymorphic cell layer and the flattened cell layer.
|
Specimen
5e:
embryonic day 18.0
(more )
Specimen
5e:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mandible |
Ambiguous |
|
5e
|
Expression disappeared in the chondrocytes of the lower half of the hypertrophic cell layer of the condylar cartilage of the mandible.
|
Specimen
6d:
embryonic day 13.0
(more )
Specimen
6d:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 13.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
6i:
embryonic day 14.0
(more )
Specimen
6i:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 14.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|