Probe:	Lhx5 cDNA5
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present	Regionally restricted	3A	Expression is present in the most anterior portion of the neural plate.
TS12: future hindbrain	Present	Regionally restricted	3A	Expression is present in restricted regions.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present		3B	Expression is present in neural epithelium of the headfold.
TS13: optic pit	Absent		3B

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present		3C	Expression is present in the headfold.
TS13: optic eminence	Absent		3C

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		3D	Expression delineates the boundary between the midbrain and forebrain.
TS15: future midbrain	Absent		3D
TS15: diencephalon	Present	Widespread	3D	Expression is present as a broad band.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		3F	Expression delineates the boundary between the midbrain and forebrain.
TS15: future midbrain	Absent		3F

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	4A left	Expression is present in the headfold; the strongest signals are detected at the anterior headfold.
TS13: optic pit	Absent		4A left

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Non-Uniform	4B left	Expression is strongest in the anterior, posterior and ventral edges of the headfold, the future ventral telencephalon and part of the diencephalon; it is weak in the dorsal edge.
TS13: optic eminence	Absent		4B left

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	4C left	Expression is present in two small groups of cells.
TS13: future prosencephalon	Present	Regionally restricted	4C left	Expression is restricted to the edge of the headfold and does not extend beyond.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present		4D left
TS14: future midbrain	Absent		4D left

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		5A right
TS15: embryo	Present	Regionally restricted	5A right	Expression is not detected in tissues outside the central nervous system.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	5B right	Expression is present in ventral midbrain, with a strip extending dorsally.
TS17: hindbrain	Present	Regionally restricted	5B right	Expression is present as two lightly stained strips runnning along the ventral portion of the longitudinal axis, with darkly stained bands segmentally arranged perpendicular to the stripes.
TS17: future spinal cord	Strong	Regionally restricted	5B right	Expression is present as three strongly stained stripes running along the entire course of the cord.
TS17: forebrain	Present		5B right

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	5C	Expression is present in ventral midbrain, with a strip extending dorsally.
TS17: hindbrain	Present	Regionally restricted	5C	Expression is present as two lightly stained strips runnning along the ventral portion of the longitudinal axis, with darkly stained bands segmentally arranged perpendicular to the stripes.
TS17: forebrain	Present		5C

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	5D right	Expression is present three groups of cells distributed dorsolaterally at each side of the spinal cord; expression is not present in the lateral motor column.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present		7A	Expression is present in the headfolds.
TS13: future hindbrain	Present		7A

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Absent		7B
TS13: future hindbrain	Present		7B