Slc1a3 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:32068 Sutherland ML, et al., Glutamate transporter mRNA expression in proliferative zones of the developing and adult murine CNS. J Neurosci. 1996 Apr 1;16(7):2191-207
Assay type:	RNA in situ
MGI Accession ID:	MGI:5467788
Gene symbol:	Slc1a3
Gene name:	solute carrier family 1 (glial high affinity glutamate transporter), member 3

Probe:	Eaat1 oligo
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Results

3A ©

Specimen 3A: embryonic day 15.0 (more

)

Structure	Level	Image
TS23: cerebellum	Present	3A
TS23: midbrain ventricular layer	Present	3A
TS23: telencephalon ventricular layer	Present	3A

3B ©

Specimen 3B: embryonic day 17.0 (more

)

Structure	Level	Image
TS25: cerebellum	Present	3B
TS25: midbrain ventricular layer	Present	3B
TS25: telencephalon ventricular layer	Present	3B

3C ©

Specimen 3C: embryonic day 19.0 (more

)

Structure	Level	Image
TS26: cerebellum	Present	3C
TS26: midbrain ventricular layer	Present	3C
TS26: telencephalon ventricular layer	Present	3C

4A/B ©

Specimen 4A/B: embryonic day 19.0 (more

)

Structure	Level	Pattern	Image	Note
TS26: dorsal grey horn	Present		4A/B
TS26: ventral grey horn	Weak		4A/B

5A/B ©

Specimen 5A/B: embryonic day 19.0 (more

)

Structure	Level	Pattern	Image	Note
TS26: olfactory cortex ventricular layer	Present		5A/B

5D/E ©

Specimen 5D/E: embryonic day 19.0 (more

)

Structure	Level	Pattern	Image	Note
TS26: telencephalon ventricular layer	Present		5D/E

5G/H ©

Specimen 5G/H: embryonic day 19.0 (more

)

Structure	Level	Pattern	Image	Note
TS26: midbrain ventricular layer	Present		5G/H

6A ©

Specimen 6A: postnatal day 0 (more

)

Structure	Level	Pattern	Image
TS27: forebrain	Weak	Diffuse	6A
TS27: cerebellar cortex	Strong		6A
TS27: hippocampus	Present		6A
TS27: medial habenular nucleus	Present		6A
TS27: corpus callosum	Weak		6A
TS27: fimbria hippocampus	Weak		6A

6B ©

Specimen 6B: postnatal day 7 (more

)

Structure	Level	Pattern	Image	Note
TS28: forebrain	Weak	Diffuse	6B
TS28: cerebellar cortex	Strong		6B
TS28: globus pallidus	Absent		6B	There is a discrepancy between the table and the text. The text states that expression is absent at P7, the table indicates weak expression.

6C ©

Specimen 6C: postnatal day 14 (more

)

Structure	Level	Pattern	Image	Note
TS28: forebrain	Present	Diffuse	6C
TS28: cerebellar cortex	Strong		6C

6D ©

Specimen 6D: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: forebrain	Weak	Diffuse	6D
TS28: cerebellar cortex	Strong		6D

7A/B ©

Specimen 7A/B: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	7A/B	Expression is in the superficial cell layer.

7E/F ©

Specimen 7E/F: postnatal day 14 (more

)

Structure	Level	Image
TS28: cortical layer II	Strong	7E/F
TS28: cortical layer III	Strong	7E/F
TS28: cortical layer IV	Strong	7E/F

9A/C ©

Specimen 9A/C: postnatal day 0 (more

)

Structure	Level	Image
TS27: dentate gyrus granule cell layer	Present	9A/C
TS27: dentate gyrus hilus	Present	9A/C
TS27: hippocampus stratum lacunosum	Present	9A/C
TS27: hippocampus stratum oriens	Present	9A/C
TS27: hippocampus stratum radiatum	Present	9A/C
TS27: hippocampus pyramidal cell layer	Absent	9A/C

9E/G ©

Specimen 9E/G: postnatal day 14 (more

)

Structure	Level	Image
TS28: hippocampus stratum lacunosum	Moderate	9E/G
TS28: hippocampus stratum oriens	Moderate	9E/G
TS28: hippocampus stratum radiatum	Moderate	9E/G
TS28: dentate gyrus granule cell layer	Weak	9E/G

10A/C ©

Specimen 10A/C: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus hilus	Present	Regionally restricted	10A/C	Expression is in a single layer of hilar interneurons bordering the inner dentate granule cell layer.
TS28: hippocampus stratum lacunosum	Present		10A/C
TS28: hippocampus stratum oriens	Present		10A/C
TS28: dentate gyrus granule cell layer	Weak		10A/C

11A/C ©

Specimen 11A/C: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present	Regionally restricted	11A/C	Expression is in migrating cells.

11B/D ©

Specimen 11B/D: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		11B/D
TS28: cerebellum granule cell layer	Present	Single cells	11B/D

11E ©

Specimen 11E: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong	Single cells	11E	Expression is in Bergmann glial cells.

12A/B ©

Specimen 12A/B: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: corpus callosum	Present		12A/B

Specimen Table 1 E15.0: embryonic day 15.0 (more

)

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Very strong
TS23: cerebral cortex ventricular layer	Very strong
TS23: olfactory lobe	Very strong
TS23: pons	Strong
TS23: hippocampus	Moderate
TS23: medulla oblongata	Moderate
TS23: spinal cord	Moderate
TS23: cerebral cortex	Weak
TS23: cerebral cortex intermediate zone	Weak
TS23: hypothalamus	Weak
TS23: midbrain ventricular layer	Weak
TS23: inferior colliculus	Absent
TS23: superior colliculus	Absent
TS23: telencephalon septum	Absent

Specimen Table 1 E17.0: embryonic day 17.0 (more

)

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Very strong
TS25: cerebral cortex ventricular zone	Very strong
TS25: midbrain ventricular layer	Strong
TS25: pons	Strong
TS25: spinal cord	Strong
TS25: hippocampus	Moderate
TS25: inferior colliculus	Moderate
TS25: medulla oblongata	Moderate
TS25: olfactory lobe	Moderate
TS25: superior colliculus	Moderate
TS25: cerebral cortex	Weak
TS25: cerebral cortex intermediate zone	Weak
TS25: hypothalamus	Weak
TS25: telencephalon septum	Weak
TS25: thalamus	Absent

Specimen Table 1 E19.0: embryonic day 19.0 (more

)

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Very strong
TS26: cerebral cortex ventricular zone	Very strong
TS26: midbrain ventricular layer	Strong
TS26: pons	Strong
TS26: spinal cord	Strong
TS26: hippocampus	Moderate
TS26: medulla oblongata	Moderate
TS26: olfactory lobe	Moderate
TS26: cerebral cortex intermediate zone	Weak
TS26: hypothalamus	Weak
TS26: midbrain	Weak
TS26: telencephalon septum	Weak
TS26: inferior colliculus	Absent
TS26: superior colliculus	Absent
TS26: thalamus	Absent

Specimen Table 2 1 Day: postnatal day 1 (more

)

Structure	Level	Pattern	Note
TS27: cerebellum Purkinje cell layer	Strong
TS27: cerebral cortex	Strong	Regionally restricted	Expression is in the lateral ventricular zone/subependymal zone.
TS27: cortical layer II	Moderate
TS27: cortical layer III	Moderate
TS27: cortical layer IV	Moderate
TS27: dentate gyrus hilus	Moderate
TS27: fimbria hippocampus	Moderate
TS27: hippocampus molecular layer	Moderate
TS27: cerebellum internal granule cell layer	Weak
TS27: corpus callosum	Weak
TS27: cortical layer V	Weak
TS27: cortical layer VI	Weak
TS27: dentate gyrus	Weak
TS27: entorhinal cortex	Weak
TS27: hippocampus stratum lacunosum	Weak
TS27: hippocampus stratum oriens	Weak
TS27: hippocampus stratum radiatum	Weak
TS27: medial habenular nucleus	Weak
TS27: presubiculum	Weak
TS27: forebrain	Weak		Expression is in the lateral, medial, and triangular septum.
TS27: retrohippocampal cortex	Weak	Regionally restricted	Parasubiculum.
TS27: accumbens nucleus	Absent
TS27: anterior dorsal thalamic nucleus	Absent
TS27: caudate-putamen	Absent
TS27: cerebellum external granule cell layer	Absent
TS27: cerebellum molecular layer	Absent
TS27: dorsal lateral geniculate nucleus	Absent
TS27: globus pallidus	Absent
TS27: hippocampus CA1	Absent
TS27: hippocampus CA2	Absent
TS27: hippocampus CA3	Absent
TS27: inferior colliculus	Absent
TS27: reticular thalamic nucleus	Absent
TS27: superior colliculus	Absent
TS27: ventral lateral geniculate nucleus	Absent
TS27: ventral posterior medial thalamic nucleus	Absent

Specimen Table 2 7 Day: postnatal day 7 (more

)

Structure	Level	Pattern	Note
TS28: cerebellum Purkinje cell layer	Very strong
TS28: accumbens nucleus	Weak
TS28: anterior dorsal thalamic nucleus	Weak
TS28: caudate-putamen	Weak
TS28: cerebellum internal granule cell layer	Weak
TS28: cerebellum molecular layer	Weak
TS28: cortical layer II	Weak
TS28: cortical layer III	Weak
TS28: cortical layer IV	Weak
TS28: cortical layer V	Weak
TS28: cortical layer VI	Weak
TS28: dentate gyrus	Weak
TS28: dentate gyrus hilus	Weak
TS28: entorhinal cortex	Weak
TS28: hippocampus CA1	Weak
TS28: hippocampus CA2	Weak
TS28: hippocampus CA3	Weak
TS28: hippocampus molecular layer	Weak
TS28: hippocampus stratum lacunosum	Weak
TS28: hippocampus stratum oriens	Weak
TS28: hippocampus stratum radiatum	Weak
TS28: medial habenular nucleus	Weak
TS28: presubiculum	Weak
TS28: cerebral cortex	Weak	Regionally restricted	Absent in the lateral ventricular zone/subependymal zone.
TS28: retrohippocampal cortex	Weak	Regionally restricted	Parasubiculum.
TS28: globus pallidus	Weak		There is a discrepancy between the table and the text. The text states that expression is absent at P7, the table indicates weak expression.
TS28: forebrain	Weak	Regionally restricted	Expression is in the lateral, medial, and triangular septum.
TS28: cerebellum external granule cell layer	Absent
TS28: corpus callosum	Absent
TS28: dorsal lateral geniculate nucleus	Absent
TS28: fimbria hippocampus	Absent
TS28: inferior colliculus	Absent
TS28: reticular thalamic nucleus	Absent
TS28: superior colliculus	Absent
TS28: ventral lateral geniculate nucleus	Absent
TS28: ventral posterior medial thalamic nucleus	Absent

Specimen Table 2 14 Day: postnatal day 14 (more

)

Structure	Level	Pattern	Note
TS28: cerebellum Purkinje cell layer	Very strong
TS28: anterior dorsal thalamic nucleus	Moderate
TS28: hippocampus molecular layer	Moderate
TS28: accumbens nucleus	Weak
TS28: caudate-putamen	Weak
TS28: cerebellum internal granule cell layer	Weak
TS28: cerebellum molecular layer	Weak
TS28: cortical layer II	Weak
TS28: cortical layer III	Weak
TS28: cortical layer IV	Weak
TS28: cortical layer V	Weak
TS28: cortical layer VI	Weak
TS28: dentate gyrus	Weak
TS28: dentate gyrus hilus	Weak
TS28: entorhinal cortex	Weak
TS28: fimbria hippocampus	Weak
TS28: globus pallidus	Weak
TS28: hippocampus stratum lacunosum	Weak
TS28: hippocampus stratum oriens	Weak
TS28: hippocampus stratum radiatum	Weak
TS28: medial habenular nucleus	Weak
TS28: presubiculum	Weak
TS28: ventral posterior medial thalamic nucleus	Weak
TS28: cerebral cortex	Weak	Regionally restricted	Absent in the lateral ventricular zone/subependymal zone.
TS28: retrohippocampal cortex	Weak	Regionally restricted	Parasubiculum.
TS28: forebrain	Weak	Regionally restricted	Expression is in the lateral, medial, and triangular septum.
TS28: cerebellum external granule cell layer	Absent
TS28: corpus callosum	Absent
TS28: dorsal lateral geniculate nucleus	Absent
TS28: hippocampus CA1	Absent
TS28: hippocampus CA2	Absent
TS28: hippocampus CA3	Absent
TS28: inferior colliculus	Absent
TS28: reticular thalamic nucleus	Absent
TS28: superior colliculus	Absent
TS28: ventral lateral geniculate nucleus	Absent

Specimen Table 2 Adult: postnatal adult (more

)

Structure	Level	Pattern	Note
TS28: cerebellum Purkinje cell layer	Very strong
TS28: accumbens nucleus	Weak
TS28: anterior dorsal thalamic nucleus	Weak
TS28: caudate-putamen	Weak
TS28: cerebellum internal granule cell layer	Weak
TS28: cerebellum molecular layer	Weak
TS28: cortical layer II	Weak
TS28: cortical layer III	Weak
TS28: cortical layer IV	Weak
TS28: cortical layer V	Weak
TS28: cortical layer VI	Weak
TS28: dentate gyrus	Weak
TS28: dentate gyrus hilus	Weak
TS28: entorhinal cortex	Weak
TS28: fimbria hippocampus	Weak
TS28: globus pallidus	Weak
TS28: hippocampus molecular layer	Weak
TS28: hippocampus stratum lacunosum	Weak
TS28: hippocampus stratum oriens	Weak
TS28: hippocampus stratum radiatum	Weak
TS28: presubiculum	Weak
TS28: ventral posterior medial thalamic nucleus	Weak
TS28: cerebral cortex	Weak	Regionally restricted	Absent in the lateral ventricular zone/subependymal zone.
TS28: retrohippocampal cortex	Weak	Regionally restricted	Parasubiculum.
TS28: forebrain	Weak	Regionally restricted	Expression is in the lateral, medial, and triangular septum.
TS28: cerebellum external granule cell layer	Absent
TS28: corpus callosum	Absent
TS28: dorsal lateral geniculate nucleus	Absent
TS28: hippocampus CA1	Absent
TS28: hippocampus CA2	Absent
TS28: hippocampus CA3	Absent
TS28: inferior colliculus	Absent
TS28: medial habenular nucleus	Absent
TS28: reticular thalamic nucleus	Absent
TS28: superior colliculus	Absent
TS28: ventral lateral geniculate nucleus	Absent

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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