Acta2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:32678 Li L, et al., SM22 alpha, a marker of adult smooth muscle, is expressed in multiple myogenic lineages during embryogenesis. Circ Res. 1996 Feb;78(2):188-95
Assay type:	RNA in situ
MGI Accession ID:	MGI:5467741
Gene symbol:	Acta2
Gene name:	actin alpha 2, smooth muscle, aorta

Probe:	SM-alpha actin cDNA
Probe preparation:	Antisense labelled with S35 RNA

Results

Specimen 5A: embryonic day 8.0 (more

)

Structure	Level	Pattern	Image	Note
TS12: bulbus cordis	Strong		5A
TS12: primitive ventricle	Strong		5A

Specimen 5B: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: bulbus cordis	Strong		5B
TS13: primitive ventricle	Strong		5B
TS13: dorsal aorta	Present	Regionally restricted	5B	Expression is in the aortic sac.

Specimen 5C: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: bulbus cordis	Strong		5C
TS15: primitive ventricle	Strong		5C
TS15: dorsal aorta	Present		5C
TS15: myotome	Present		5C
TS15: blood vessel	Present	Regionally restricted	5C	Expression is in cranial vessels.

Specimen 5D: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		5D
TS17: myotome	Present		5D
TS17: primitive ventricle	Present		5D
TS17: umbilical artery	Present		5D
TS17: arterial system	Present	Regionally restricted	5D	Expression is in cranial vessels.

Specimen 6A: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: heart	Present		6A

Specimen 6B: embryonic day 13.5 (more

)

Structure	Level	Image
TS21: myotome	Present	6B
TS21: skeletal muscle	Present	6B
TS21: heart	Absent	6B

Specimen 6C: embryonic day 14.5 (more

)

Structure	Level	Image
TS22: myotome	Present	6C
TS22: skeletal muscle	Present	6C
TS22: heart	Absent	6C

Specimen 6D: embryonic day 15.5 (more

)

Structure	Level	Image	Note
TS23: diaphragm	Very strong	6D
TS23: intercostal muscle	Very strong	6D
TS23: skeletal muscle	Strong	6D	Intense in head and neck muscles.
TS23: lung	Present	6D
TS23: smooth muscle tissue	Present	6D
TS23: stomach	Present	6D

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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