Gene Expression Data
Assay Details
Assay
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Reference:
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J:43464
Matise MP, et al., Expression patterns of developmental control genes in normal and Engrailed-1 mutant mouse spinal cord reveal early diversity in developing interneurons. J Neurosci. 1997 Oct 15;17(20):7805-16
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5467562
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Gene symbol:
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En1
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Gene name:
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engrailed 1
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Results
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Specimen
8b:
embryonic day 11.5; En1tm2Alj/En1 +
(more )
Note: Lower thoracic spinal cord.
Specimen
8b:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lower thoracic spinal cord.
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Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
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8b
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Expression was detected in interneurons migrating out of the ventricular zone and ventrally. Expression was detected in cells near the marginal zone lateral to motoneurons in the lateral motor column.
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Specimen
8c:
embryonic day 11.5; En1tm1Alj/ En1tm2Alj
(more )
Note: Lower thoracic spinal cord.
Specimen
8c:
(close )
Genetic Background:
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either: (involves: 129 * CD-1) or (involves: 129 * C57BL/6J * CD-1)
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Mutant Allele(s):
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En1tm1Alj/En1tm2Alj
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lower thoracic spinal cord.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
Regionally restricted |
8c
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Expression was detected in interneurons migrating out of the ventricular zone and ventrally. Expression was detected in cells near the marginal zone lateral to motoneurons in the lateral motor column. Expression was indistinguishable from wild type.
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Specimen
not shown:
embryonic day 15.5; En1tm2Alj/En1 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS23: spinal cord lateral wall |
Present |
Regionally restricted |
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Expression was detected in cells located near the marginal zone lateral to motoneurons in the lateral motor column.
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Specimen
not shown:
(close )
Genetic Background:
|
either: (involves: 129 * CD-1) or (involves: 129 * C57BL/6J * CD-1)
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Mutant Allele(s):
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En1tm1Alj/En1tm2Alj
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: spinal cord lateral wall |
Present |
Regionally restricted |
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Expression was detected in cells located near the marginal zone lateral to motoneurons in the lateral motor column. Expression was indistinguishable from wild type.
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