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Gene Expression Data
Assay Details
Assay
Reference: J:43464 Matise MP, et al., Expression patterns of developmental control genes in normal and Engrailed-1 mutant mouse spinal cord reveal early diversity in developing interneurons. J Neurosci. 1997 Oct 15;17(20):7805-16
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5467562
Gene symbol: En1
Gene name: engrailed 1
Reporter: lacZ
Antibody: anti-B-galactosidase (5Prime3Prime)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 8b: embryonic day 11.5; En1tm2Alj/En1+ (more )
Note: Lower thoracic spinal cord.
Structure Level Pattern Image Note
TS19: future spinal cord Present 8b Expression was detected in interneurons migrating out of the ventricular zone and ventrally. Expression was detected in cells near the marginal zone lateral to motoneurons in the lateral motor column.

Specimen 8c: embryonic day 11.5; En1tm1Alj/En1tm2Alj (more )
Note: Lower thoracic spinal cord.
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 8c Expression was detected in interneurons migrating out of the ventricular zone and ventrally. Expression was detected in cells near the marginal zone lateral to motoneurons in the lateral motor column. Expression was indistinguishable from wild type.

Specimen not shown: embryonic day 15.5; En1tm2Alj/En1+ (more )
Structure Level Pattern Image Note
TS23: spinal cord lateral wall Present Regionally restricted Expression was detected in cells located near the marginal zone lateral to motoneurons in the lateral motor column.

Specimen not shown: embryonic day 15.5; En1tm1Alj/En1tm2Alj (more )
Structure Level Pattern Image Note
TS23: spinal cord lateral wall Present Regionally restricted Expression was detected in cells located near the marginal zone lateral to motoneurons in the lateral motor column. Expression was indistinguishable from wild type.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory