Gene Expression Data
Assay Details
Assay
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Reference:
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J:43464
Matise MP, et al., Expression patterns of developmental control genes in normal and Engrailed-1 mutant mouse spinal cord reveal early diversity in developing interneurons. J Neurosci. 1997 Oct 15;17(20):7805-16
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5467548
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Gene symbol:
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En1
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Gene name:
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engrailed 1
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Antibody:
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alpha Enhb-1
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Present |
Regionally restricted |
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Ventrally located cells were detected at the lateral margins of the spinal cord.
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Specimen
3a:
embryonic day 11.5
(more )
Note: Section from the lumbar region.
Specimen
3a:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Section from the lumbar region.
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Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
Regionally restricted |
3a
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Expression was detected lateral and medial to motoneurons in the lumbar region.
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Specimen
3b:
embryonic day 11.5
(more )
Note: Section from the midthoracic region.
Specimen
3b:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Section from the midthoracic region.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
Regionally restricted |
3b
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Expression was detected throughout the ventrolateral ventral horn in the midthoracic region.
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Specimen
3c:
embryonic day 15.5
(more )
Note: Section from the lower thoracic region.
Specimen
3c:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Section from the lower thoracic region.
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Structure |
Level |
Pattern |
Image |
Note |
TS23: ventral grey horn |
Present |
Regionally restricted |
3c
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Expression was detected in widely dispersed interneurons in the ventrolateral gray matter of the spinal cord.
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