Gene Expression Data
Assay Details
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Assay
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Reference:
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J:43464
Matise MP, et al., Expression patterns of developmental control genes in normal and Engrailed-1 mutant mouse spinal cord reveal early diversity in developing interneurons. J Neurosci. 1997 Oct 15;17(20):7805-16
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5467510
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Gene symbol:
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Jag1
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Gene name:
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jagged 1
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Results
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Specimen
1d:
embryonic day 11.5; En1tm2Alj/En1 +
(more  )
Note: Double labeled: purple - Jag1; blue - En1 reporter (assay MGI:5467409)
Specimen
1d:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Double labeled: purple - Jag1; blue - En1 reporter (assay MGI:5467409)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain ventricular layer |
Present |
Regionally restricted |
1d
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The expression domain in the posterior hindbrain ventricular zone corresponds to the domain of neuroepithelium from which En1 expressing cells derive. The rostral limit of the ventral expression was at the rhombomere 1-2 border.
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Specimen
not shown:
embryonic day 10.5; En1tm2Alj/En1 +
(more )
Note: Also stained for En1 reporter (assay MGI:5467409)
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Also stained for En1 reporter (assay MGI:5467409)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
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The expression domain in the ventricular zone corresponds to the domain of neuroepithelium from which En1 expressing cells derive.
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| TS17: hindbrain ventricular layer |
Present |
Regionally restricted |
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The expression domain in the ventricular zone corresponds to the domain of neuroepithelium from which En1 expressing cells derive.
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Specimen
not shown:
embryonic day 11.5; En1tm2Alj/En1 +
(more )
Note: Also stained for En1 reporter (assay MGI:5467409)
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Agarose
|
|
Note:
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Also stained for En1 reporter (assay MGI:5467409)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
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The expression domain in the ventricular zone corresponds to the domain of neuroepithelium from which En1 expressing cells derive.
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| TS19: midbrain |
Present |
Regionally restricted |
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The rostral expression boundary of the dorsal stripe was in the midbrain.
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Specimen
1e:
embryonic day 11.5
(more )
Note: Also stained for Lmx1b (assay MGI:5467415); both purple.
Specimen
1e:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Also stained for Lmx1b (assay MGI:5467415); both purple.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain ventricular layer |
Present |
Regionally restricted |
1e
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Expression was detected in two domains, ventral and dorsal. The dorsal-most stripe was located just ventral to the sulcus limitans and to Lmx1b expression.
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