Gene Expression Data
Assay Details
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Assay
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Reference:
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J:1430
Hutchins JB, et al., Developmental distribution of platelet-derived growth factor in the mouse central nervous system. Brain Res Dev Brain Res. 1992 Jun 19;67(2):121-35
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5467256
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Gene symbol:
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Pdgfa
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Gene name:
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platelet derived growth factor, alpha
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Antibody:
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anti-PDGF(AB)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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The antibody used in this assay recognizes the heterodimer.
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Results
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Specimen
3C:
embryonic day 12.5
(more  )
Note: transverse section
Specimen
3C:
(close  )
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Genetic Background:
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either: (CBA x C57BL)F1 or ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: basal columns |
Present |
Regionally restricted |
3C
,
3D
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Expression is present in the future ventral horn.
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Specimen
4C:
embryonic day 13.5
(more )
Note: parasagittal section
Specimen
4C:
(close )
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Genetic Background:
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either: (CBA x C57BL)F1 or ICR
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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parasagittal section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: basal columns |
Present |
Regionally restricted |
4C
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Expression is present in the ventral spinal cord.
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| TS21: dorsal root ganglion |
Present |
Widespread |
4D
,
4C
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| TS21: spinal nerve |
Present |
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4C
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Expression is present in peripheral nerves coursing between the vertebrae. It is also present in dorsal roots entering the spinal cord.
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Specimen
6A:
embryonic day 15.0
(more )
Note: parasagittal section
Specimen
6A:
(close )
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Genetic Background:
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either: (CBA x C57BL)F1 or ICR
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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parasagittal section
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Specimen
6B:
embryonic day 15.0
(more )
Note: parasagittal section
Specimen
6B:
(close )
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Genetic Background:
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either: (CBA x C57BL)F1 or ICR
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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parasagittal section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: spinal cord |
Present |
Regionally restricted |
6B
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Expression is present in a column in the ventrolateral spinal cord. Cells in both horns express.
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| TS23: brainstem |
Present |
Regionally restricted |
6B
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Expression is present in fibrous elements, as is illustrated in the inset.
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Specimen
6E:
embryonic day 15.0
(more )
Specimen
6E:
(close )
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Genetic Background:
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either: (CBA x C57BL)F1 or ICR
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: trigeminal ganglion |
Present |
Single cells |
6E
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Expression is present in only a few cells.
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Analysis Tools