Probe:	Otlx2 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: oral region	Present	Regionally restricted		Expressed in the oral epithelium/ectoderm.

Structure	Level	Pattern	Image	Note
TS15: oral region	Present	Regionally restricted	2A	Expressed in the oral ectoderm.
TS15: 1st branchial arch maxillary component mesenchyme	Strong		2A	Expressed in the maxillary mesenchyme just posterior to the optic cup.
TS15: future midbrain	Weak	Regionally restricted	2A	Faint expression in the basal plate just rostral to the mesencephalic flexure.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Regionally restricted		Expressed in the mesenchyme at the basis of the forelimb.

Structure	Level	Pattern	Image	Note
TS17: oral epithelium	Present		2B	Expressed in the oral ectoderm.
TS17: eye mesenchyme	Present		2B
TS17: dermomyotome	Weak		2B
TS17: forelimb bud mesenchyme	Weak		2B
TS17: trunk	Weak		2B
TS17: midbrain	Strong	Regionally restricted	2B	Expressed in the basal plate, and extended from the middle part of the mesencephalon to the future mammillary region.
TS17: diencephalon	Present	Regionally restricted	2B	Expressed as a small triangular dorsal extension in the middiencephalic region, prefiguring the zona limitans intrathalamica.

Structure	Level	Pattern	Image	Note
TS19: forebrain	Present	Regionally restricted	3A	Expression is restricted to the mammillary area (basal prosomere 4) and extends to the ventricular zone.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Graded	3B	Expressed in a clear rostrocaudal gradient. Expressed in the basal plate of the anterior part in two layers.
TS19: forebrain	Strong	Regionally restricted	3B	Expressed in the basal plate of the caudal prosencephalon (prosomere 1-4).
TS19: metencephalon basal plate	Present	Regionally restricted	3B	Expressed in the first rhombomere.

Structure	Level	Pattern	Image	Note
TS19: metencephalon basal plate	Present	Regionally restricted	3C	Expressed in the first rhombomere.
TS19: midbrain	Present	Regionally restricted	3C	Expressed in the mesencephalic alar (future superior colliculus). Expression is strongest at the mesencephalic/prosomere1 boundary and declines caudally.
TS19: diencephalon	Present	Regionally restricted	3C	Expressed in a thin dorsal extension at the prosomere 2/3 boundary corresponding to the zona limitans.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	3D	Expressed in the superficial alar plate (future superior colliculus), and the subventricular basal plate. The two domains are discontinuous with each other.
TS20: metencephalon basal plate	Present	Regionally restricted	3D	Expressed in the first rhombomere.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present		4A
TS20: midbrain	Present	Regionally restricted	4A	Expressed in the alar plate of the rostral region (future superior colliculus), and the basal plate. The two domains are discontinuous with each other.
TS20: metencephalon basal plate	Present	Regionally restricted	4A	Expressed in the first rhombomere.
TS20: forebrain	Present	Regionally restricted	4A	Homogeneously strong expression in the prosencephalon, with a sharp rostal limit at the prosomeric 4/5 boundary.

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	4B	Expressed in the area dorsal to the motoneurons, but not in the ventricular layer where proliferating neuroblasts are found.

Structure	Level	Pattern	Image	Note
TS19: neural tube lateral wall	Present	Regionally restricted		Expressed as a thin column along the entire length of the spinal cord, dorsal to the motor columns. No expression in the ventricular layer where proliferating neuroblasts are found.

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Regionally restricted	5A	Expression extends to the pretectal area. The caudal limit of the expression in the mesencephalic basal plate has retracted.
TS21: diencephalon	Present	Regionally restricted	5A	A fainter and more diffuse expression in the alar plate of prosomere 1. Expressed in the entire mammillary area (basal prosomere 4). Expressed in a thin dorsal extension at the prosomere 2/3 boundary corresponding to the zona limitans.
TS21: metencephalon basal plate	Present	Regionally restricted	5A	Expressed in the first rhombomere.

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Regionally restricted	5B	The caudal limit of the expression in the mesencephalic basal plate has retracted.
TS21: diencephalon	Present	Regionally restricted	5B	Expressed in the entire mammillary area (basal prosomere 4), including the ventral midline. Expressed in a thin dorsal extension at the prosomere 2/3 boundary corresponding to the zona limitans.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	5C	Expressed in the entire mammillary area (basal prosomere 4).

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	5D	Expressed in the entire mammillary area (basal prosomere 4), including the ventral midline. Expressed in a thin dorsal extension at the prosomere 2/3 boundary corresponding to the zona limitans.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	6A	Expressed in three patches underlining the prosomere 2/3, prosomere 3/4, and prosomere 4/5 boundaries.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	6B	Expressed in the mamillary region.
TS24: zona incerta	Present	Regionally restricted	6B	Expressed in the dorsal part of the zona incerta in continuity with a thin dorsal extension corresponding to the zona limitans
TS24: zona limitans intrathalamica	Present		6B
TS24: prethalamus	Present		6B	Expressed in the subthalamic nucleus.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	6C	Expressed in the mamillary region.
TS24: zona incerta	Present	Regionally restricted	6C	Expressed in the dorsal part of the zona incerta in continuity with a thin dorsal extension corresponding to the zona limitans
TS24: zona limitans intrathalamica	Present		6C
TS24: midbrain tegmentum	Present		6C	Expression in prerubral tegmentum.
TS24: pretectum	Present		6C
TS24: superior colliculus	Present		6C
TS24: prethalamus	Present		6C	Expressed in the subthalamic nucleus.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	6D	Expressed in the prerubral tegmentum, pretectum, superior colliculus, and mamillary region.

Structure	Level	Pattern	Image	Note
TS27: diencephalon	Present	Regionally restricted	8A	Expressed along the zona limitans, probably corresponding to the intergeniculate leaflet and the deep part of the thalamic reticular nucleus.
TS27: subthalamic nucleus	Present		8A
TS27: zona incerta	Present	Regionally restricted	8A	Expressed in the zona incerta pars dorsalis.

Structure	Level	Pattern	Image	Note
TS27: subthalamic nucleus	Present		8B
TS27: zona incerta	Present	Regionally restricted	8B	Expressed in the zona incerta pars dorsalis.

Structure	Level	Pattern	Image	Note
TS27: diencephalon	Present	Regionally restricted	8C	Expressed in the prerubral tegmentum.

Structure	Level	Pattern	Image	Note
TS15: Rathke's pouch	Present		9A	Expressed in the roof of the stomatodeum.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present	Regionally restricted	9B	Expressed in a ring-like pattern.

Structure	Level	Pattern	Image	Note
TS19: Rathke's pouch	Present	Uniform	9C	Homogeneously expressed.

Structure	Level	Pattern	Image	Note
TS21: adenohypophysis pars anterior	Weak	Uniform	9D	Homogeneously expressed in the anterior lobe.
TS21: adenohypophysis pars intermedia	Weak	Uniform	9D	Homogeneously expressed in the intermediate lobe.

Structure	Level	Pattern	Image	Note
TS24: adenohypophysis pars anterior	Weak	Uniform	9E	Homogeneously expressed in the anterior lobe.
TS24: adenohypophysis pars intermedia	Weak	Uniform	9E	Homogeneously expressed in the intermediate lobe.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme	Present