Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:2959
Price M, et al., Regional expression of the homeobox gene Nkx-2.2 in the developing mammalian forebrain. Neuron. 1992 Feb;8(2):241-55
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5462556
|
Gene symbol:
|
Nkx2-1
|
Gene name:
|
NK2 homeobox 1
|
Probe:
|
TTF1 probe1
|
Probe preparation:
|
Antisense RNA
|
Visualized with:
|
Autoradiography
|
|
Results
|
Specimen
4C:
embryonic day 12.5
(more )
Specimen
4C:
(close )
Genetic Background:
|
involves: C57BL/6 * SJL
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hypothalamus |
Present |
Regionally restricted |
4C
|
Expression was detected in the posterior area.
|
TS20: corpus striatum |
Present |
Regionally restricted |
4C
|
Expression was detected in the median corpus striatum.
|
Specimen
5A TTF-1:
embryonic day 10.0
(more )
Note: Section pseudocolored and overlaid with other expression patterns. Colors: green - Nkx2-1; blue - Dlx1 (assay MGI:5462557); red - Nkx2-2 (assay MGI:5462555).
Specimen
5A TTF-1:
(close )
Genetic Background:
|
involves: C57BL/6 * SJL
|
Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Section pseudocolored and overlaid with other expression patterns. Colors: green - Nkx2-1; blue - Dlx1 (assay MGI:5462557); red - Nkx2-2 (assay MGI:5462555).
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: diencephalon |
Present |
|
6C
,
6A merge
,
5A TTF1
,
6B
,
6D/E
,
6A TTF-1
|
Expression was detected throughout the neuroepithelium.
|
Specimen
5B TTF-1:
embryonic day 11.0
(more )
Specimen
5B TTF-1:
(close )
Genetic Background:
|
involves: C57BL/6 * SJL
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: diencephalon |
Present |
Regionally restricted |
5B TTF1
|
Expression was detected throughout the walls of the posterior hypothalamus.
|
Specimen
6F/G:
embryonic day 10.0
(more )
Specimen
6F/G:
(close )
Genetic Background:
|
involves: C57BL/6 * SJL
|
Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: diencephalon |
Present |
Regionally restricted |
6F/G
|
Expression was detected throughout the neuroepithelium.
|
Specimen
7A TTF-1:
embryonic day 12.5
(more )
Note: Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
Specimen
7A TTF-1:
(close )
Genetic Background:
|
involves: C57BL/6 * SJL
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
|
Specimen
7B TTF-1:
embryonic day 12.5
(more )
Note: Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
Specimen
7B TTF-1:
(close )
Genetic Background:
|
involves: C57BL/6 * SJL
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: thalamus |
Absent |
|
7B TTF1
|
|
TS20: hypothalamus |
Present |
Regionally restricted |
7B TTF1
|
Expression was detected in posterior hypothalamus.
|
Specimen
7C TTF-1:
embryonic day 12.5
(more )
Note: Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
Specimen
7C TTF-1:
(close )
Genetic Background:
|
involves: C57BL/6 * SJL
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: thalamus |
Absent |
|
7C TTF1
|
|
TS20: hypothalamus |
Present |
Regionally restricted |
7C TTF1
|
Expression was detected in the floor of posterior hypothalamus.
|
Specimen
7D TTF-1:
embryonic day 12.5
(more )
Note: Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
Specimen
7D TTF-1:
(close )
Genetic Background:
|
involves: C57BL/6 * SJL
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
|
Specimen
9A TTF-1:
embryonic day 14.5
(more )
Specimen
9A TTF-1:
(close )
Genetic Background:
|
involves: C57BL/6 * SJL
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hypothalamus |
Strong |
Regionally restricted |
9A TTF1
|
Expression was detected in ventral hypothalamus.
|
Specimen
9B TTF-1:
embryonic day 14.5
(more )
Specimen
9B TTF-1:
(close )
Genetic Background:
|
involves: C57BL/6 * SJL
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: thalamus |
Present |
Regionally restricted |
9B TTF1
|
Expression was detected in the ependymal layer.
|
TS22: hypothalamus |
Present |
Regionally restricted |
9B TTF1
|
Expression was weak in the ependyma, but was strong in the most dorsal hypothalamic area.
|
|