Gene Expression Data
Assay Details
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Assay
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Reference:
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J:2959
Price M, et al., Regional expression of the homeobox gene Nkx-2.2 in the developing mammalian forebrain. Neuron. 1992 Feb;8(2):241-55
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5462555
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Gene symbol:
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Nkx2-2
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Gene name:
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NK2 homeobox 2
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Probe:
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probe b
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Probe preparation:
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Antisense RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
4A/B:
embryonic day 12.5
(more  )
Specimen
4A/B:
(close  )
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Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus |
Present |
Regionally restricted |
4A/B
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Expression was detected in a centrally located narrow band.
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| TS20: thalamus |
Present |
Regionally restricted |
4A/B
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Expression was detected in a narrow band in the ventral thalamus and in the most posterior part of the dorsal thalamus.
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Specimen
5A Nkx-2.2:
embryonic day 10.0
(more )
Note: Section pseudocolored and overlaid with other expression patterns. Colors: red - Nkx2-2; blue - Dlx1 (assay MGI:5462557); green - Nkx2-1 (assay MGI:5462556).
Specimen
5A Nkx-2.2:
(close )
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Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Section pseudocolored and overlaid with other expression patterns. Colors: red - Nkx2-2; blue - Dlx1 (assay MGI:5462557); green - Nkx2-1 (assay MGI:5462556).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: diencephalon |
Present |
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6A Nkx-2.2
,
6C
,
6B
,
5A Nkx2.2
,
6A merge
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Expression was detected throughout the neuroepithelium.
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| TS16: future hindbrain |
Weak |
Regionally restricted |
6B
,
6C
,
5A Nkx2.2
,
6A Nkx-2.2
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6A merge
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Expression was detected in the myelencephalon.
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Specimen
5B Nkx-2.2:
embryonic day 11.0
(more )
Specimen
5B Nkx-2.2:
(close )
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Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: diencephalon |
Present |
Regionally restricted |
5B Nkx2.2
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Expression was detected in an intermediate domain in the hypothalamus.
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Specimen
7A Nkx-2.2:
embryonic day 12.5
(more )
Note: Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
Specimen
7A Nkx-2.2:
(close )
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Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
7A Nkx2.2
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Expression was detected in a narrow band of cells immediately above the zona limitans intrathalamica. The domain extends laterally to the walls of the diencephalon.
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Specimen
7B Nkx-2.2:
embryonic day 12.5
(more )
Note: Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
Specimen
7B Nkx-2.2:
(close )
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Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
7B Nkx2.2
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Expression was detected in a narrow band of cells immediately above the zona limitans intrathalamica. The domain extends laterally to the walls of the diencephalon.
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Specimen
7C Nkx-2.2:
embryonic day 12.5
(more )
Note: Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
Specimen
7C Nkx-2.2:
(close )
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Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus |
Strong |
Regionally restricted |
7C Nkx2.2
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Expression was detected in the hypothalamic paraventricular areas, but not in the floor of the hypothalamus.
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Specimen
7D Nkx-2.2:
embryonic day 12.5
(more )
Note: Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
Specimen
7D Nkx-2.2:
(close )
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Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Fig. 7 shows a frontal series of sections through the forebrain, with A being more anterior and D more posterior.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Absent |
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7D Nkx2.2
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There is no expression in the very posterior parts of the diencephalon.
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Specimen
8A Nkx-2.2:
embryonic day 12.5
(more )
Specimen
8A Nkx-2.2:
(close )
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Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: thalamus |
Present |
Regionally restricted |
8A Nkx2.2
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Expression was detected in the ventral thalamus in a ring of cells including ependymal cells.
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| TS20: diencephalon |
Present |
Regionally restricted |
8A Nkx2.2
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Expression was not detected in anterior diencephalon.
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Specimen
8B Nkx-2.2:
embryonic day 12.5
(more )
Specimen
8B Nkx-2.2:
(close )
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Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: thalamus |
Present |
Regionally restricted |
8B Nkx2.2
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Expression was detected in the ventral thalamus in a ring of cells including ependymal cells.
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| TS20: diencephalon |
Present |
Regionally restricted |
8B Nkx2.2
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Expression was not detected in anterior diencephalon.
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Specimen
8C Nkx-2.2:
embryonic day 12.5
(more )
Specimen
8C Nkx-2.2:
(close )
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Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus |
Strong |
Regionally restricted |
8C Nkx2.2
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Expression was detected centrally in an X-shaped pattern (in transverse section).
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| TS20: diencephalon |
Strong |
Regionally restricted |
8C Nkx2.2
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Expression was not detected in anterior diencephalon.
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Specimen
9A Nkx-2.2:
embryonic day 14.5
(more )
Specimen
9A Nkx-2.2:
(close )
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Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
9A Nkx2.2
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Expression was detected in 2 thin cell layers immediately above the interparencephalic boundary. Expression was abundant in lateral areas surrounding the prospective ventral corpus geniculate.
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| TS22: hypothalamus |
Present |
Regionally restricted |
9A Nkx2.2
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Expression was detected in a dorsal, paraventricular region.
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Specimen
9B Nkx-2.2:
embryonic day 14.5
(more )
Specimen
9B Nkx-2.2:
(close )
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Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
9B Nkx2.2
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Expression was abundant in lateral areas surrounding the prospective ventral corpus geniculate.
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| TS22: thalamus |
Present |
Regionally restricted |
9B Nkx2.2
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Expression was detected in the most ventral part of the dorsal thalamus.
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| TS22: hypothalamus |
Present |
Regionally restricted |
9B Nkx2.2
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Expression was detected in the ependymal layer.
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Analysis Tools