Probe:	D3 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS8: ectoplacental cone	Present		3A
TS8: extraembryonic ectoderm	Present		3A	Expression in the extraembryonic ectoderm was less than in the ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS8: ectoplacental cone	Present		3B
TS8: extraembryonic ectoderm	Present		3B	Expression in the extraembryonic ectoderm was less than in the ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS10: extraembryonic mesoderm	Present		3C	Expression was restricted to trophectoderm and mesoderm lineages.
TS10: trophectoderm	Present		3C	Expression was restricted to trophectoderm and mesoderm lineages.

Structure	Level	Pattern	Image	Note
TS10: extraembryonic mesoderm	Present		3D	Expression was restricted to trophectoderm and mesoderm lineages.
TS10: trophectoderm	Present		3D	Expression was restricted to trophectoderm and mesoderm lineages.

Structure	Level	Pattern	Image	Note
TS10: primary trophoblast giant cell	Absent		3E

Structure	Level	Pattern	Image	Note
TS10: extraembryonic mesoderm	Present		3F
TS10: yolk sac mesoderm	Present		3F
TS10: extraembryonic visceral endoderm	Absent		3F
TS10: parietal endoderm	Absent		3F

Structure	Level	Pattern	Image	Note
TS10: extraembryonic mesoderm	Present		3G
TS10: yolk sac mesoderm	Present		3G

Structure	Level	Pattern	Image	Note
TS12: head	Present

Structure	Level	Pattern	Image	Note
TS12: embryo mesenchyme	Present		6A
TS12: foregut diverticulum endoderm	Present		6A
TS12: neural ectoderm	Present		6A
TS12: embryo	Present	Regionally restricted	6A	Expression was detected in two domains. An anterior domain in neuroectoderm, mesoderm, and foregut endoderm, and a second domain in the caudal-most region of the embryo.
TS12: neural fold	Present	Regionally restricted	6A	Expression was strongly reduced in the dorsal tips of the neural folds.

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum endoderm	Present		6B
TS12: hindgut diverticulum endoderm	Present		6B
TS12: neural ectoderm	Present		6B
TS12: neural groove	Present	Regionally restricted	6B	Expression in the caudal region of the embryo was detected in the neural groove.
TS12: embryo mesenchyme	Present	Regionally restricted	6B	Expression was detected in two domains (anterior and posterior) in the embryo mesenchyme.
TS12: neural fold	Present	Regionally restricted	6B	Expression was strongly reduced in the dorsal tips of the neural folds.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		6C	In 10-12 somite embryos, the two expression domains have merged.
TS13: neural fold	Present	Regionally restricted	6C	Expression was strongly reduced in the dorsal tips of the neural folds.

Structure	Level	Pattern	Image	Note
TS13: yolk sac	Present	Regionally restricted	6D	Expression was detected in blood islands in the visceral yolk sac.

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum endoderm	Present		6E
TS12: neural ectoderm	Present		6E
TS12: neural fold	Present	Regionally restricted	6E	Expression was strongly reduced in the dorsal tips of the neural folds.

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present		6F
TS12: cardiovascular system	Present	Regionally restricted	6F	Expression was detected in red blood cells in the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS12: embryo mesenchyme	Present		6G
TS12: trunk	Present	Regionally restricted	6G	Expression was detected in the ventral half of the trunk.

Structure	Level	Pattern	Image	Note
TS12: hindgut diverticulum endoderm	Present		6H
TS12: trunk	Present	Regionally restricted	6H	Expression was detected in the ventral half of the trunk.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		6I	In 10-12 somite embryos, the two expression domains have merged.
TS13: trunk	Present	Regionally restricted	6I	Expression in the ventral trunk was progressively extended to the dorsal half with further development.

Structure	Level	Pattern	Image	Note
TS13: amnion	Present	Regionally restricted	6J	Expression was detected in amnion ectoderm and mesoderm.

Structure	Level	Pattern	Image	Note
TS12: amnion ectoderm	Present
TS12: amnion mesenchyme	Present
TS12: cardiac muscle tissue	Present
TS12: endocardial tube	Present
TS12: head mesenchyme	Present
TS12: hindgut diverticulum endoderm	Present
TS12: neural ectoderm	Present
TS12: neural tube	Present
TS12: paraxial mesenchyme	Present
TS12: somatopleure	Present
TS12: somite	Present
TS12: splanchnopleure	Present
TS12: surface ectoderm	Present
TS12: allantois	Present			Expression was detected in the base of the allantois.
TS12: blood island	Strong
TS12: foregut diverticulum endoderm	Strong
TS12: yolk sac endoderm	Absent
TS12: yolk sac mesenchyme	Ambiguous