Gene Expression Data
Assay Details
Assay
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Reference:
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J:40837
Mitsiadis TA, et al., Mouse Serrate-1 (Jagged-1): expression in the developing tooth is regulated by epithelial-mesenchymal interactions and fibroblast growth factor-4. Development. 1997 Apr;124(8):1473-83
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5461954
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Gene symbol:
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Jag1
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Gene name:
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jagged 1
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Probe:
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Ser-1 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 7.5
(more )
Specimen
1A:
(close )
Genetic Background:
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either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo endoderm |
Present |
Regionally restricted |
1A
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Expressed in a small area around the primitive streak, presumably in embryonic endoderm.
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Specimen
1B:
embryonic day 8.5
(more )
Specimen
1B:
(close )
Genetic Background:
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either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS13: rhombomere 1 |
Strong |
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1B
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High expression in two transverse stripes, and lower levels in a third, more posterior stripe.
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TS13: rhombomere 3 |
Strong |
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1B
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High expression in two transverse stripes, and lower levels in a third, more posterior stripe.
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TS13: rhombomere 5 |
Present |
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1B
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Specimen
1C:
embryonic day 9.5
(more )
Specimen
1C:
(close )
Genetic Background:
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either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: branchial arch |
Strong |
Regionally restricted |
1C
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Intense expression in a region between the first and second branchial arches, and the area just posterior of the second branchial arch.
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TS15: otocyst |
Strong |
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1C
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Intense expression in the otic vesicle.
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Specimen
not shown:
embryonic day 9.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1D:
embryonic day 8.5
(more )
Note: This embryo has 6 somites. Double labeled: blue - Jag1 (Ser-1); brown - Egr2 (Krox-20; assay MGI:5462080).
Specimen
1D:
(close )
Genetic Background:
|
either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This embryo has 6 somites. Double labeled: blue - Jag1 (Ser-1); brown - Egr2 (Krox-20; assay MGI:5462080).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: rhombomere |
Present |
Regionally restricted |
1D
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Expressed in 3 stripes corresponding to the presumptive rhombomere 1, 3 and 5.
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Specimen
1E:
embryonic day 9.5
(more )
Specimen
1E:
(close )
Genetic Background:
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either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS15: mesonephros |
Present |
|
1E
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Expressed in pronephros.
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TS15: somite |
Present |
Regionally restricted |
1E
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Expressed in a thin stripe of cells in the forming somite boundary.
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Specimen
1F:
embryonic day 11.5
(more )
Specimen
1F:
(close )
Genetic Background:
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either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1G:
embryonic day 11.5
(more )
Specimen
1G:
(close )
Genetic Background:
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either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3 E12.5:
embryonic day 12.5
(more )
Specimen
3 E12.5:
(close )
Genetic Background:
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either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3 DRG:
embryonic day 12.5
(more )
Specimen
3 DRG:
(close )
Genetic Background:
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either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3 max. proc.:
embryonic day 12.5
(more )
Specimen
3 max. proc.:
(close )
Genetic Background:
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either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3 aorta:
embryonic day 12.5
(more )
Specimen
3 aorta:
(close )
Genetic Background:
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either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4A:
embryonic day 10.5
(more )
Specimen
4A:
(close )
Genetic Background:
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either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS17: mesonephros |
Present |
Regionally restricted |
4A
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Expressed in epithelial tubules.
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Specimen
4B:
embryonic day 13.5-14.5
(more )
Specimen
4B:
(close )
Genetic Background:
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either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 13.5-14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4C:
embryonic day 15.5-16.5
(more )
Specimen
4C:
(close )
Genetic Background:
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either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 15.5-16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4D:
embryonic day 15.5-16.5
(more )
Specimen
4D:
(close )
Genetic Background:
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either: (CBA x C57BL/6)F1 or (CBA x NMRI)F1
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Age:
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embryonic day 15.5-16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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