Fgf6 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:35179 Ozawa K, et al., Expression of the fibroblast growth factor family and their receptor family genes during mouse brain development. Brain Res Mol Brain Res. 1996 Sep 5;41(1-2):279-88
Assay type:	RT-PCR
MGI Accession ID:	MGI:5461913
Gene symbol:	Fgf6
Gene name:	fibroblast growth factor 6

Probe:	Fgf6-pG, Fgf6-pH
Probe preparation:	Double stranded labelled with digoxigenin DNA
Assay notes:	The semi-quantitative RT-PCR gel was Southern blotted (probe - MGI:5461860).

Results

Image: 1_FGF-6

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	542 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
Lane 1	E12.0 (a)	TS20: hindbrain	Present (c)	1 µg; total RNA	Slc:ICR		Not Specified
Lane 2	E14.0 (a)	TS22: hindbrain	Absent (d)	1 µg; total RNA	Slc:ICR		Not Specified
Lane 3	E16.0 (a)	TS24: hindbrain	Present	1 µg; total RNA	Slc:ICR		Not Specified
Lane 4	P1	TS27: hindbrain	Present (c)	1 µg; total RNA	Slc:ICR		Not Specified
Lane 5	P3	TS27: hindbrain	Present (c)	1 µg; total RNA	Slc:ICR		Not Specified
Lane 6	P5	TS28: hindbrain	Present (c)	1 µg; total RNA	Slc:ICR		Not Specified
Lane 7	P adult	TS28: telencephalon	Ambiguous	1 µg; total RNA	Slc:ICR		Not Specified
Lane 8	P adult	TS28: cerebellum	Present (c)	1 µg; total RNA	Slc:ICR		Not Specified
Lane 9	Control
Lane 10	Control
Lane 11	Control
Lane 12	Control
Lane 13	Control
Lane 14	Control

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression increased during embryonic development but decreased markedly after birth.
(c) Present assigned by curator.
(d) Absent assigned by curator.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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