Reporter:	lacZ
	Direct Detection
Assay notes:	Embryos were fixed in 1% formaldehyde/0.2% gluteraldehyde.

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Strong		1A
TS13: 1st branchial arch	Strong		1A
TS13: amnion	Weak		1A
TS13: somatopleure	Weak		1A

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Strong		1A'
TS13: 1st branchial arch	Strong		1A'
TS13: amnion	Weak		1A'
TS13: somatopleure	Weak		1A'

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Strong		1B
TS15: mesenchyme derived from somatopleure	Strong	Regionally restricted	1B	Expression is present in the ventral somatopleure.
TS15: dermomyotome	Absent		1B
TS15: somite	Absent		1B
TS15: embryo mesenchyme	Present	Single cells	1B	Expression is present in a few, isolated cells just dorsal of the ventral somatopleure.

Structure	Level	Pattern	Image	Note
TS15: somite	Absent		1B'

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Present		1C
TS15: 2nd branchial arch	Present		1C
TS15: mesenchyme derived from somatopleure	Present	Regionally restricted	1C	Expression is present in the ventral somatopleure.
TS15: forelimb bud	Present	Regionally restricted	1C	The scattered staining just dorsal of the ventral somatopleure is appearing to coalesce into an elongated structure in the dorso-medial margin of the limb bud.
TS15: somite	Present		1C	The first signs of staining are observed.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Present		1D
TS16: 2nd branchial arch	Present		1D
TS16: mesenchyme derived from somatopleure	Present	Regionally restricted	1D	Expression is present in the ventral somatopleure.
TS16: forelimb bud	Present	Regionally restricted	1D	The scattered staining just dorsal of the ventral somatopleure is appearing to coalesce into an elongated structure in the dorso-medial margin of the limb bud.
TS16: somite	Present		1D	The first signs of staining are observed.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		1E
TS17: 2nd branchial arch	Present		1E
TS17: 3rd branchial arch	Present		1E
TS17: 4th branchial arch	Present		1E
TS17: forelimb bud	Present		1E
TS17: somite	Present		1E

Structure	Level	Pattern	Image	Note
TS19: somite	Strong	Widespread	1F	There is strong expression in all somites.
TS19: 1st branchial arch	Present	Patchy	1F
TS19: 2nd branchial arch	Present	Patchy	1F
TS19: body wall mesenchyme	Present		1F	Expression is present in the somatopleure.
TS19: branchial arch mesenchyme	Present		1F	Expression is present in presumptive branchiomeric muscle precursors.
TS19: future arm mesenchyme	Present		1F	Expression is present in the developing muscle groups.
TS19: future leg mesenchyme	Present		1F	Expression is present in the developing muscle groups.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present		1G	Expression is present in the presumptive muscle anlagen.
TS20: hindlimb mesenchyme	Present		1G	Expression is present in the presumptive muscle anlagen.
TS20: septum transversum non-hepatic component	Present		1G	Expression is present in the presumptive diaphgragm anlagen.
TS20: body wall mesenchyme	Present		1G	Expression is present in the presumptive abdominal muscle anlagen.
TS20: paraxial mesenchyme	Present		1G	Expression is present in the presumptive back muscle anlagen.
TS20: head mesenchyme	Present		1G	Expression is present in the presumptive head muscle anlagen.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present		1G'	Expression is present in the presumptive muscle anlagen.

Structure	Level	Pattern	Image	Note
TS21: forelimb mesenchyme	Present		1H	Expression is present in the presumptive muscle anlagen.
TS21: hindlimb mesenchyme	Present		1H	Expression is present in the presumptive muscle anlagen.
TS21: diaphragm	Present		1H	Expression is present in the presumptive muscle anlagen.
TS21: anterior abdominal wall musculature	Present		1H	Expression is present in the presumptive muscle anlagen.
TS21: pectoral girdle and thoracic body wall musculature	Present		1H	Expression is present in the presumptive back muscle anlagen.
TS21: head mesenchyme	Present		1H	Expression is present in the presumptive head muscle anlagen.

Structure	Level	Pattern	Image	Note
TS22: forelimb mesenchyme	Present		1I	Expression is present in the presumptive muscle anlagen.
TS22: hindlimb mesenchyme	Present		1I	Expression is present in the presumptive muscle anlagen.
TS22: diaphragm	Present		1I	Expression is present in the presumptive muscle anlagen.
TS22: anterior abdominal wall musculature	Present		1I	Expression is present in the presumptive muscle anlagen.
TS22: pectoral girdle and thoracic body wall musculature	Present		1I	Expression is present in the presumptive back muscle anlagen.
TS22: cranial musculature	Present		1I	Expression is present in the presumptive muscle anlagen.

Structure	Level	Pattern	Image	Note
TS20: body wall mesenchyme	Strong		2A	Expression is present in the entire body wall (somatopleure).
TS20: paraxial mesenchyme	Present	Regionally restricted	2A	Expression is present in the intercostal muscles.
TS20: forelimb mesenchyme	Present		2A	Expression is present in the extensor and flexor muscles.

Structure	Level	Pattern	Image	Note
TS21: body wall mesenchyme	Present		2B	Expression is present in the entire body wall (somatopleure).
TS21: paraxial mesenchyme	Present	Regionally restricted	2B	Expression is present in the intercostal muscles.
TS21: forelimb musculature	Present		2B	Expression is present in the extensor and flexor muscles.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present		2C	Expression is present in the extensor and flexor muscles.

Structure	Level	Pattern	Image	Note
TS21: forelimb musculature	Present		2D	Expression is present in the extensor and flexor muscles.

Structure	Level	Pattern	Image	Note
TS20: body wall mesenchyme	Strong		2E	Expression is present in the somatopleure.
TS20: septum transversum non-hepatic component	Strong		2E	Expression is present in the diaphgragm.

Structure	Level	Pattern	Image	Note
TS21: skin	Present	Regionally restricted	2F	Expression is present in the cutaneous maximus.
TS21: anterior abdominal wall musculature	Present	Regionally restricted	2F	Expression is present in the oblique abdominis.

Structure	Level	Pattern	Image	Note
TS12: head mesenchyme	Present		2G
TS12: surface ectoderm	Present		2G
TS12: 1st branchial arch	Present		2G

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch ectoderm	Present		2H
TS15: 2nd branchial arch ectoderm	Present		2H
TS15: 1st branchial arch mesenchyme	Present	Regionally restricted	2H	Expression is present in an internal pocket of cells.
TS15: 2nd branchial arch mesenchyme	Present	Regionally restricted	2H	Expression is present in an internal pocket of cells.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	2I	Expression is present in the masseter, which is derived from the first branchial arch.

Structure	Level	Pattern	Image	Note
TS21: head mesenchyme	Present	Regionally restricted	2J	Expression is present in the masseter, which is derived from the first branchial arch.

Structure	Level	Pattern	Image	Note
TS20: upper arm rest of mesenchyme	Present	Regionally restricted		Expression is present in the presumptive biceps brachii and triceps brachii.
TS20: skin	Present	Regionally restricted		Expression is present in the presumptive cutaneous maximus.
TS20: septum transversum non-hepatic component	Present			Expression is present in the presumptive diaphragm.
TS20: limb mesenchyme	Present			Expression is present in the presumptive extensor and flexor muscles.
TS20: hindlimb mesenchyme	Present			Expression is present in the presumptive hindlimb muscles, including the semitendinosus and tibialis anterior.
TS20: paraxial mesenchyme	Present	Regionally restricted		Expression is present in the following prospective muscles: gluteus; latissimus dorsi; oblique abdominis; pectoralis; rectus abdominis; spinodeltoid; acromiotrapezius; deep back; intercostal; serratus; and spinotrapezius.
TS20: head mesenchyme	Present	Regionally restricted		Expression is present in the presumptive digastric, orbicularis oculi, masseter, sternohyoid, sternomastoid, and temporalis muscles.
TS20: outer ear	Present	Regionally restricted		Expression is present in the presumptive external ear muscle.
TS20: integumental system	Present	Regionally restricted		Expression is present in the presumptive platysma muscle.
TS20: eye	Present	Regionally restricted		Expression is present in the presumptive extraocular muscle.
TS20: tongue muscle	Present

Structure	Level	Pattern	Image	Note
TS21: forelimb musculature	Present	Regionally restricted		Expression is present in the presumptive biceps brachii and triceps brachii.
TS21: skin	Present	Regionally restricted		Expression is present in the presumptive cutaneous maximus.
TS21: diaphragm	Present
TS21: limb musculature	Present			Expression is present in the presumptive extensor and flexor muscles.
TS21: hindlimb musculature	Present			Muscles with expression include the semitendinosus and tibialis anterior.
TS21: vertebral axis musculature	Present	Regionally restricted		Areas of expression include the following presumptive muscles: gluteus; deep back; intercostal; and serratus.
TS21: pectoral girdle and thoracic body wall musculature	Present	Regionally restricted		Expression is present in the presumptive latissimus dorsi, pectoralis, acromiotrapezius, and spinotrapezius.
TS21: anterior abdominal wall musculature	Present	Regionally restricted		Expression is present in the presumptive oblique abdominis and rectus abdominis.
TS21: deltoid pre-muscle mass	Present			Expression is present in the presumptive spinodeltoid.
TS21: outer ear	Present	Regionally restricted		Expression is present in the presumptive external ear muscle.
TS21: head mesenchyme	Present	Regionally restricted		Expression is present in the presumptive digastric, orbicularis oculi, masseter, sternohyoid, sternomastoid, and temporalis muscles.
TS21: integumental system	Present	Regionally restricted		Expression is present in the presumptive platysma muscle.
TS21: extrinsic ocular pre-muscle mass	Present
TS21: tongue muscle	Present

Structure	Level	Pattern	Image	Note
TS22: forelimb musculature	Present	Regionally restricted		Expression is present in the presumptive biceps brachii and triceps brachii.
TS22: skin	Present	Regionally restricted		Expression is present in the presumptive cutaneous maximus.
TS22: diaphragm	Present
TS22: limb musculature	Present			Expression is present in the presumptive extensor and flexor muscles.
TS22: hindlimb musculature	Present			Muscles with expression include the semitendinosus and tibialis anterior.
TS22: pelvic girdle musculature	Present	Regionally restricted		Expression is present in the presumptive gluteus muscle.
TS22: latissimus dorsi	Present
TS22: anterior abdominal wall musculature	Present	Regionally restricted		Expression is present in the presumptive oblique abdominis.
TS22: pectoral girdle and thoracic body wall muscle	Present	Regionally restricted		Expression is present in the presumptive pectoralis.
TS22: rectus abdominis	Present
TS22: deltoid	Present	Regionally restricted		Expression is present in the spinodeltoid.
TS22: trapezius	Present			Expression is present in the acromiotrapezius and spinotrapezius.
TS22: axial musculature	Present	Regionally restricted		Expression is present in the presumptive deep back muscles and the serratus.
TS22: intercostal muscle	Present
TS22: cranial muscle	Present	Regionally restricted		Expression is present in the presumptive digastric, orbicularis oculi, and sternohyoid muscles.
TS22: outer ear	Present	Regionally restricted		Expression is present in the presumptive external ear muscle.
TS22: masseter	Present
TS22: platysma	Present
TS22: sterno-mastoid	Present
TS22: temporalis	Present
TS22: tongue muscle	Present

Structure	Level	Pattern	Image	Note
TS28: biceps brachii	Present
TS28: skin muscle	Present	Regionally restricted		Expression is present in the cutaneous maximus.
TS28: diaphragm	Present
TS28: limb musculature	Present	Regionally restricted		Expression is present in the extensor and flexor muscles.
TS28: hindlimb musculature	Present			Muscles with expression include the caudofemoralis.
TS28: biceps femoris	Present
TS28: gluteal muscle	Present
TS28: latissimus dorsi	Present
TS28: pectoralis	Present
TS28: oblique abdominal muscle	Present
TS28: rectus abdominis	Present
TS28: semitendinosus	Present
TS28: deltoid	Present	Regionally restricted		Expression is present in the spinodeltoid.
TS28: tibialis anterior	Present
TS28: triceps brachii	Present
TS28: trapezius	Present			Expression is present in the acromiotrapezius and spinotrapezius.
TS28: back musculature	Present			Expression is present in deep back muscles.
TS28: intercostal muscle	Present
TS28: serratus muscle	Present
TS28: digastric	Present
TS28: pinna muscle	Present
TS28: orbicularis oculi	Present
TS28: masseter	Present
TS28: platysma	Present
TS28: sternohyoid muscle	Present
TS28: sterno-mastoid	Present
TS28: temporalis	Present
TS28: extrinsic ocular muscle	Present
TS28: tongue muscle	Present

Structure	Level	Pattern	Image	Note
TS28: cranial muscle	Present	Widespread	5A	Expression is present in all.
TS28: digastric	Present		5A
TS28: extrinsic ocular muscle	Present		5A
TS28: masseter	Present		5A
TS28: orbicularis oculi	Present		5A
TS28: pinna muscle	Present		5A
TS28: sterno-mastoid	Present		5A
TS28: sternohyoid muscle	Present		5A
TS28: temporalis	Present		5A

Structure	Level	Pattern	Image	Note
TS28: forelimb musculature	Present	Widespread	5B	Expression is present in the majority of hypaxial muscles in the forelimb and most of the shoulder muscles.
TS28: deltoid	Present		5B
TS28: trapezius	Present		5B
TS28: triceps brachii	Present	Regionally restricted	5B	There is no expression in the lateral head.

Structure	Level	Pattern	Image	Note
TS28: hindlimb musculature	Present	Widespread	5C	Expression is present in the majority of hypaxial muscles of the hindlimb and hip muscles.
TS28: gluteal muscle	Present		5C
TS28: quadriceps femoris muscle	Present		5C
TS28: tensor fasciae lata	Absent		5C

Structure	Level	Pattern	Image	Note
TS28: tongue muscle	Present		5D

Structure	Level	Pattern	Image	Note
TS28: trunk musculature	Present	Widespread	5E	Expression is present in all.
TS28: deltoid	Present		5E
TS28: latissimus dorsi	Present		5E
TS28: trapezius	Present		5E

Structure	Level	Pattern	Image	Note
TS28: trunk musculature	Present	Widespread	5F	Expression is present in all.
TS28: intercostal muscle	Present		5F
TS28: serratus muscle	Present		5F

Structure	Level	Pattern	Image	Note
TS28: abdomen musculature	Strong	Widespread	5G	Expression is present in the majority of muscles.
TS28: oblique abdominal muscle	Present		5G
TS28: rectus abdominis	Present		5G

Structure	Level	Pattern	Image	Note
TS28: abdomen musculature	Strong	Widespread	5H	Expression is present in the majority of muscles.
TS28: oblique abdominal muscle	Present		5H
TS28: transversus abdominis	Absent		5H