Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Pvalbtm1.1(cre/ERT2)Hze
Name:	parvalbumin; targeted mutation 1.1, Hongkui Zeng
MGI ID:	MGI:5461312
Synonyms:	Pvalb-2A-CreERT2, Pvalb-2A-CreERT2-Deltahygro, Pvalbtm1.1(cre/ERT2)Htz
Gene:	Pvalb  Location: Chr15:78075314-78090600 bp, - strand  Genetic Position: Chr15, 36.93 cM, cytoband E
Alliance:	Pvalbtm1.1(cre/ERT2)Hze page

Germline Transmission:	Earliest citation of germline transmission: J:191262
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Pvalbtm1.1(cre/ERT2)Hze expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Pvalb (MGI:97821)   Mutation details: Embryonic stem (ES) cells, previously targeted with a T2A-Cre vector immediately downstream of the parvalbumin translational STOP codon were re-targeted with a T2A-CreERT2 vector and a FLP-expressing plasmid to facilitate recombination. Correctly targeted ES cells had (from 5' to 3') a three amino acid linker sequence, an frt3 site within Pvalb intron 3, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A sequence that is in-frame with the Pvalb coding sequence, a CreERT2 fusion gene, the Pvalb 3' UTR sequence from exon 4, an attB site, a PGK-hygromycin-SV40polyA cassette and an attP site. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the attB/attP-flanked PGK cassette. The resulting mice were bred with C57BL/6J wildtype mice for at least two additional generations (and the PhiC31 transgene was removed). (J:155793, J:191262, J:220523)

Activity:	Tissue activity of this recombinase allele
Driver:	Pvalb (mouse) Summary of all recombinase alleles driven by Pvalb. Comparative matrix view of recombinase activities Alliance mouse Pvalb gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pvalb Mutation:	29 strains or lines available

Original:	J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
All:	7 reference(s)