Probe:	E2A probe
Probe preparation:	Antisense labelled with digoxigenin
Visualized with:	Alkaline phosphatase
Assay notes:	Authors indicate that from E9.5-12.5 identical results were obtained for two Msx1as probes used in in situ hybridization assays. The two probes were I (MGI:4948297) and E2A (MGI:4948293).

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component	Absent		3B
TS15: 1st branchial arch maxillary component	Absent		3B
TS15: future brain	Present		3B

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component	Absent		3E
TS15: 1st branchial arch maxillary component	Absent		3E

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	3H	Expression was detected in the proximal part of the mandible.
TS17: brain	Present		3H
TS17: limb	Present		3H

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	3K	Expression was detected in the proximal part of the mandible.

Structure	Level	Pattern	Image	Note
TS19: mandible primordium	Present	Graded	3N	Expression was detected in almost all the oral part of the mandible. An expression gradient was observed with levels higher in the proximal region.
TS19: brain	Present		3N
TS19: limb	Present		3N

Structure	Level	Pattern	Image	Note
TS19: mandible primordium	Present	Graded	3Q	Expression was detected in almost all the oral part of the mandible. An expression gradient was observed with levels higher in the proximal region.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	4A	Expression was detected in the proximal regions of the mandibular arch.

Structure	Level	Pattern	Image	Note
TS21: jaw epithelium	Present	Regionally restricted	5I	Expression was detected in the dental epithelium.
TS21: jaw mesenchyme	Weak	Regionally restricted	5I	Low level expression was detected in the dental mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: jaw epithelium	Present		5N	Expression was detected in the dental epithelium.

Structure	Level	Pattern	Image	Note
TS27: jaw	Present	Regionally restricted	5S	Expression was detected in dental mesenchyme and dental epithelium.

Structure	Level	Pattern	Image	Note
TS15: limb	Absent		6B

Structure	Level	Pattern	Image	Note
TS17: limb	Present	Regionally restricted	6E	Expression was detected in the proximal region immediately adjacent to the Msx1 Sense transcript.

Structure	Level	Pattern	Image	Note
TS19: limb	Present	Regionally restricted	6H	Expression was detected in the region adjacent to the Msx1 Sense transcript.

Structure	Level	Pattern	Image	Note
TS22: limb interdigital region	Present	Regionally restricted	6J	Expression was detected in the proximal part of the interdigital regions.