Antibody:	anti- cyclin E (M-20) (sc-481)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Strong	Regionally restricted	2B	Expressed in the neuroepithelium, excluding the deepest layer where dividing cells are present.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present		2D
TS20: cortical preplate	Present		2D

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Present		2G
TS22: cerebral cortex ventricular layer	Present		2G
TS22: cortical plate	Present		2G	Decreased expression compared with E12.5.
TS22: cerebral cortex marginal layer	Present		2G	Decreased expression compared with E12.5. Some cells showed intense expression.

Structure	Level	Pattern	Image	Note
TS27: neocortex layer	Present		2J	Detected in a number of cells in the cortical plate.
TS27: cerebral cortex ventricular zone	Trace	Single cells	2J	Expressed in few cells.
TS27: cortical layer I	Trace	Single cells	2J	Expressed in occasional cells in the most superficial aspect of the molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		2M	Detected in the nuclei, cytoplasm and apical processes of pyramidal neurons. Also expressed in glial cells.
TS28: cortical layer III	Present		2M	Detected in the nuclei, cytoplasm and apical processes of pyramidal neurons. Also expressed in glial cells.
TS28: cortical layer IV	Present		2M	Detected in the nuclei, cytoplasm and apical processes of pyramidal neurons. Also expressed in glial cells.
TS28: cortical layer VI	Present		2M	Detected in the nuclei, cytoplasm and apical processes of pyramidal neurons. Also expressed in glial cells.
TS28: cortical layer V	Present		2M' , 2M	Detected in the nuclei, cytoplasm and apical processes of pyramidal neurons. Also expressed in glial cells.
TS28: cerebral cortex ventricular zone	Absent		2M	Expression was hardly present.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present	Widespread	2P	Expressed in the whole cerebellar primordium.
TS22: cerebellum ventricular layer	Present		2P	Highest in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Strong		2R

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		2U	Intense expression was also present in the processes of Bergmann glia.