Antibody:	anti-Sox2
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS19: oral epithelium	Present	Regionally restricted	5A	Expression is present in the oral epithelium and in most cells of the dental epithelium of developing incisors.
TS19: jaw primordium mesenchyme	Present	Single cells	5A	Expression is present in a limited number of cells adjacent to the epithelium in developing incisors.

Structure	Level	Pattern	Image	Note
TS21: incisor epithelium	Present		5C , 5B	Expression is present in most cells.
TS21: jaw epithelium	Present		5C , 5B	Expression is present in the vestibular lamina.
TS21: incisor mesenchyme	Present	Single cells	5B , 5C	Expression is present in a limited number of cells adjacent to the epithelium.

Structure	Level	Pattern	Image	Note
TS22: incisor enamel organ	Strong	Non-Uniform	5E , 5D	There is abundant staining in the labial cervical loop area.
TS22: cervical loop	Strong	Regionally restricted	5E , 5D	There is abundant staining in the labial cervical loop area.
TS22: jaw epithelium	Strong		5D , 5F	Expression is present in the vestibular lamina.
TS22: incisor mesenchyme	Present	Single cells	5D , 5E	Expression is present in a limited number of cells adjacent to the epithelium.

Structure	Level	Pattern	Image	Note
TS24: incisor enamel organ	Present	Regionally restricted	5I , 5H , 5G	Expression is restricted in the labial cervical loop area, including the basal epithelium, stellate reticulum, and outer enamel epithelium. There is also some expression in the transit-amplifying cells. It is not present in the lingual cervical loop.
TS24: cervical loop	Present	Regionally restricted	5H , 5I , 5G	Expression is restricted in the labial cervical loop area and is not present in the lingual cervical loop.
TS24: outer enamel epithelium	Present		5G , 5I , 5H
TS24: stellate reticulum	Present		5G , 5I , 5H
TS24: incisor mesenchyme	Present	Single cells	5G , 5I	Expression is present in some mesenchymal cells adjacent to the labial cervical loop.

Structure	Level	Pattern	Image	Note
TS26: incisor enamel organ	Present	Regionally restricted	5K , 5L , 5J	Expression is restricted in the labial cervical loop area, including the basal epithelium, stellate reticulum, and outer enamel epithelium. There is also some expression in the transit-amplifying cells. It is not present in the lingual cervical loop.
TS26: cervical loop	Present	Regionally restricted	5J , 5L , 5K	Expression is restricted in the labial cervical loop area and is not present in the lingual cervical loop.
TS26: outer enamel epithelium	Present		5L , 5K , 5J
TS26: stellate reticulum	Present		5L , 5K , 5J
TS26: incisor mesenchyme	Present	Single cells	5J , 5L	Expression is present in some mesenchymal cells adjacent to the labial cervical loop.

Structure	Level	Pattern	Image	Note
TS27: incisor enamel organ	Present	Regionally restricted	5O , 5N , 5M	Expression is restricted in the labial cervical loop area, including the basal epithelium, stellate reticulum, and outer enamel epithelium. There is also some expression in the transit-amplifying cells. It is not present in the lingual cervical loop.
TS27: cervical loop	Present	Regionally restricted	5M , 5N , 5O	Expression is restricted in the labial cervical loop area and is not present in the lingual cervical loop.
TS27: outer enamel epithelium	Present		5O , 5N , 5M
TS27: stellate reticulum	Present		5O , 5N , 5M
TS27: incisor dental papilla	Present		5M , 5O	Expression is present in some mesenchymal cells adjacent to the labial cervical loop.

Structure	Level	Pattern	Image	Note
TS28: incisor enamel organ	Present	Regionally restricted	5P , 5Q , 5R	Expression is restricted in the labial cervical loop area, including the basal epithelium, stellate reticulum, and outer enamel epithelium. There is also some expression in the transit-amplifying cells. It is not present in the lingual cervical loop.
TS28: cervical loop	Present	Regionally restricted	5R , 5Q , 5P	Expression is restricted in the labial cervical loop area and is not present in the lingual cervical loop.
TS28: outer enamel epithelium	Present		5R , 5P , 5Q
TS28: stellate reticulum	Present		5R , 5P , 5Q
TS28: incisor dental papilla	Present	Single cells	5R , 5P	Expression is present in some mesenchymal cells adjacent to the labial cervical loop.

Structure	Level	Pattern	Image	Note
TS28: incisor enamel organ	Present	Regionally restricted	5T , 5U , 5S	Expression is restricted in the labial cervical loop area, including the basal epithelium, stellate reticulum, and outer enamel epithelium. There is also some expression in the transit-amplifying cells. It is not present in the lingual cervical loop.
TS28: cervical loop	Present	Regionally restricted	5S , 5T , 5U	Expression is restricted in the labial cervical loop area and is not present in the lingual cervical loop.
TS28: outer enamel epithelium	Present		5T , 5S , 5U
TS28: stellate reticulum	Present		5T , 5S , 5U
TS28: incisor dental papilla	Present	Single cells	5U , 5S	Expression is present in some mesenchymal cells adjacent to the labial cervical loop.

Structure	Level	Pattern	Image	Note
TS19: oral epithelium	Strong	Regionally restricted	6A	Expression is present in the oral epithelium and the dental epithelium of developing molars.
TS19: jaw primordium mesenchyme	Present	Single cells	6A	Expression is present in a limited number of cells adjacent to the epithelium in developing molars.

Structure	Level	Pattern	Image	Note
TS21: molar epithelium	Strong	Non-Uniform	6B	There is strong expression in the lingual side of the dental epithelium; there is only weak staining in the buccal side.
TS21: molar mesenchyme	Present	Single cells	6B	Expression is present in a limited number of cells adjacent to the epithelium in developing molars.

Structure	Level	Pattern	Image	Note
TS22: molar enamel organ	Present	Regionally restricted	6C	Thre is strong staining in the lingual side of the tooth germ. It is also expressed in the stellate reticulum. There is no staining in the primary enamel knot.
TS22: stellate reticulum	Present		6C
TS22: primary enamel knot	Absent		6C
TS22: molar mesenchyme	Present	Single cells	6C	Expression is present in a limited number of cells adjacent to the epithelium in developing molars.

Structure	Level	Pattern	Image	Note
TS24: inner enamel epithelium	Present	Regionally restricted	6F , 6D , 6E	Expression is present in the lingual side of the outer enamel epithelium, the cervical loop, and the inner enamel epithelium between secondary enamel knots.
TS24: molar enamel organ	Present	Regionally restricted	6F , 6D , 6E	Expression is present in the lingual side of the outer enamel epithelium, the cervical loop, and the inner enamel epithelium between secondary enamel knots.
TS24: outer enamel epithelium	Present	Regionally restricted	6F , 6D , 6E	Expression is present in the lingual side of the outer enamel epithelium, the cervical loop, and the inner enamel epithelium between secondary enamel knots.
TS24: cervical loop	Present		6E , 6D , 6F
TS24: molar mesenchyme	Present	Single cells	6D	Expression is present in a limited number of cells adjacent to the epithelium in developing molars.

Structure	Level	Pattern	Image	Note
TS26: molar enamel organ	Present	Regionally restricted	6G , 6I , 6H	Expression is present in the stratum intermedium, the stellate reticulum adjacent to stratum intermedium layer, and the inner enamel epithelium, especially the cervical loop area.
TS26: stellate reticulum	Present	Regionally restricted	6I , 6H , 6G	Expression is seen adjacent to the stratum intermedium layer.
TS26: cervical loop	Present		6I , 6H , 6G
TS26: inner enamel epithelium	Present		6I , 6H , 6G
TS26: stratum intermedium	Present		6I , 6H , 6G

Structure	Level	Pattern	Image	Note
TS27: molar enamel organ	Present	Regionally restricted	7C , 7B , 7A	Expression is present in the stratum intermedium, stellate reticulum adjacent to stratum intermedium layer, and some inner enamel epithelium cells within the cervical loop area.
TS27: stratum intermedium	Present		7B , 7C , 7A
TS27: stellate reticulum	Present	Regionally restricted	7B , 7C , 7A	Expression is seen adjacent to the stratum intermedium layer.
TS27: cervical loop	Present	Regionally restricted	7B , 7C , 7A	Expression is seen in some inner enamel epithelium cells within the cervical loop area.
TS27: inner enamel epithelium	Present	Regionally restricted	7B , 7C , 7A	Expression is seen in some inner enamel epithelium cells within the cervical loop area.

Structure	Level	Pattern	Image	Note
TS28: Hertwig's epithelial root sheath	Present	Regionally restricted	7E , 7D , 7F	Expression is present in the apical end of Hertwig's epithelium root sheath.
TS28: molar enamel organ	Present	Regionally restricted	7E , 7D , 7F	Expression is present in the apical end of Hertwig's epithelium root sheath.

Structure	Level	Pattern	Image	Note
TS28: Hertwig's epithelial root sheath	Present	Regionally restricted	7G , 7H	Expression is present in the apical end of Hertwig's epithelium root sheath.
TS28: molar enamel organ	Present	Regionally restricted	7G , 7H	Expression is present in the apical end of Hertwig's epithelium root sheath.
TS28: molar dental pulp	Present	Regionally restricted	7H , 7G	Expression is present in the perivascular region of the dental pulp.

Structure	Level	Pattern	Image	Note
TS28: molar dental pulp	Present	Regionally restricted	7J , 7K	Expression is present in the perivascular region of the dental pulp.

Structure	Level	Pattern	Image	Note
TS28: incisor	Present			The protein is seen in the tooth. However, it is not present in the differentiated odontoblasts and ameloblasts, although the mRNA is seen in those cells.
TS28: molar	Present			The protein is seen in the tooth. However, it is not present in the differentiated odontoblasts and ameloblasts, although the mRNA is seen in those cells.

Structure	Level	Pattern	Image	Note
TS19: eye	Strong	Widespread	S1D

Structure	Level	Pattern	Image	Note
TS19: brain	Strong	Widespread	S1E

Structure	Level	Pattern	Image	Note
TS26: hair follicle	Present	Regionally restricted	S1F	Expression is confined to a subset of cells in the tip of the dermal papilla.