Gene Expression Data
Assay Details
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Assay
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Reference:
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J:23910
Cifuentes-Diaz C, et al., M-cadherin localization in developing adult and regenerating mouse skeletal muscle: possible involvement in secondary myogenesis. Mech Dev. 1995 Mar;50(1):85-97
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5450823
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Gene symbol:
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Cdh15
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Gene name:
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cadherin 15
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Antibody:
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Anti-M-cadherin
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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Two antibodies were described. The antibody used in this assay could have been MGI:5450812 or MGI:5450815.
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Results
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Specimen
2A:
embryonic day 12.5
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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129P1/ReJ
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: upper leg |
Weak |
Regionally restricted |
2A
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Weak, discontinous expression was detected in myogenic cells, primary myoblasts and myotubes, of developing hindlimb thigh muscle.
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Specimen
2B:
embryonic day 14.5
(more )
Specimen
2B:
(close )
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Genetic Background:
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129P1/ReJ
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: upper leg |
Weak |
Regionally restricted |
2B
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The expression in developing hindlimb thigh muscle was weaker than at E12.5.
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Specimen
2C:
embryonic day 16.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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129P1/ReJ
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: upper leg muscle |
Present |
Widespread |
2C
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Expression level increased at this stage and was noted throughout the muscle. Immunostaining outlined the surface of myotubes and accumulated at myotube-myotube contact sites.
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Specimen
2D:
embryonic day 18.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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129P1/ReJ
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: upper leg muscle |
Present |
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2D
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Staining delineated the areas of contact between smaller and larger myotubes.
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Specimen
not shown:
postnatal day 0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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129P1/ReJ
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: upper leg muscle |
Present |
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Staining did not change from that seen at E18.5.
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Specimen
2E:
postnatal day 2
(more )
Specimen
2E:
(close )
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Genetic Background:
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129P1/ReJ
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: upper leg muscle |
Weak |
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2E
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Expression was very weak except at a few sites of contact between myogenic cells and myotubes.
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Specimen
not shown:
postnatal day 7
(more )
Specimen
not shown:
(close )
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Genetic Background:
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129P1/ReJ
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
3A:
embryonic day 18.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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129P1/ReJ
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hindlimb musculature |
Present |
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3A
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Immunostaining was homogeneously distributed on the sarcolemma of both large diameter primary and smaller diameter secondary myotubes, which were in clusters. Expression was also noted at the sites of contact between primary myotubes and myoblasts.
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Specimen
3B,3B":
embryonic day 16.5
(more )
Specimen
3B,3B":
(close )
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Genetic Background:
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129P1/ReJ
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hindlimb musculature |
Present |
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3B
,
3B"
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Expression was specifically accumulated at areas of contact between primary and secondary myotubes.
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Specimen
3C,3C":
embryonic day 16.5
(more )
Specimen
3C,3C":
(close )
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Genetic Background:
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129P1/ReJ
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hindlimb musculature |
Present |
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3C"
,
3C
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Expression was noted at the areas of contact between myotubes encircled by the same basal lamina.
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Specimen
5B:
postnatal adult
(more )
Specimen
5B:
(close )
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Genetic Background:
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129P1/ReJ
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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