Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:116150
Torban E, et al., Tissue, cellular and sub-cellular localization of the Vangl2 protein during embryonic development: Effect of the Lp mutation. Gene Expr Patterns. 2007 Jan;7(3):346-54
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5449371
|
|
Gene symbol:
|
Vangl2
|
|
Gene name:
|
VANGL planar cell polarity 2
|
|
Antibody:
|
Vangl2 antibody
|
|
Detection system:
|
Secondary antibody coupled to Cy3
|
|
|
Results
|
Specimen
5A, C:
embryonic day 10.5
(more  )
Note: Double labeled: red - Vangl2; green - Ctnnb1 (assay MGI:5449368). (C) Merged image of (A) and (B).
Specimen
5A, C:
(close  )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Vangl2; green - Ctnnb1 (assay MGI:5449368). (C) Merged image of (A) and (B).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
5A
,
5C
|
Staining is at the plasma membrane. Inset is a higher magnification of the boxed area.
|
Specimen
5D, F:
(close )
|
Genetic Background:
|
involves: 101/Rl * C3H/Rl
|
|
Mutant Allele(s):
|
Vangl2Lp-m1Jus/Vangl2Lp-m1Jus
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Vangl2; green - Ctnnb1 (assay MGI:5449368). (F) Merged image of (D) and (E).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Weak |
Regionally restricted |
5D
,
5F
|
Staining is intracellular in this mutant. Inset is a higher magnification of the boxed area.
|
Specimen
S1 A,B:
embryonic day 14.5
(more )
Specimen
S1 A,B:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea |
Present |
Regionally restricted |
S1B
,
S1A
|
Expression is in the hair cells of the organ of Corti at the plasma membrane.
|
Specimen
S1 E:
embryonic day 14.5
(more )
Specimen
S1 E:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea |
Present |
Regionally restricted |
S1E
|
Expression is in the hair cells of the organ of Corti at the plasma membrane.
|
Specimen
S1 F:
(close )
|
Genetic Background:
|
involves: A
|
|
Mutant Allele(s):
|
Vangl2Lp/Vangl2Lp
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea |
Present |
Regionally restricted |
S1F
|
Expression is in the hair cells of the organ of Corti but not at the plasma membrane.
|
Specimen
S1 G:
embryonic day 14.5
(more )
Specimen
S1 G:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea |
Present |
Regionally restricted |
S1G
|
Expression is in the hair cells of the organ of Corti at the plasma membrane.
|
Specimen
S1 H:
(close )
|
Genetic Background:
|
involves: A
|
|
Mutant Allele(s):
|
Vangl2Lp/Vangl2Lp
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea |
Present |
Regionally restricted |
S1H
|
Expression is in the hair cells of the organ of Corti but not at the plasma membrane.
|
|
Analysis Tools