Probe:	miR-183 LNA probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS14: otic placode	Absent

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Widespread	1C	Broad expression within the otic vesicle

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present		1F	Expression in the entire otic vesicle, cochleo-vestibular ganglion, and all cells of the neural tube.
TS19: inner ear	Present		1F	Expression in the entire otic vesicle, cochleo-vestibular ganglion, and all cells of the neural tube.
TS19: neural tube	Present		1F	Expression in the entire otic vesicle, cochleo-vestibular ganglion, and all cells of the neural tube.

Structure	Level	Pattern	Image	Note
TS20: inner ear vestibular component	Present	Widespread	2C	Expression in the entire vestibule.

Structure	Level	Pattern	Image	Note
TS20: inner ear vestibular component	Present	Widespread	2F	Expression in all different cells of the epithelium.

Structure	Level	Pattern	Image	Note
TS22: inner ear vestibular component	Present	Regionally restricted	2I	Expression restricted to hair cells of the luminal layer of the vestibule. No expression in the surrounding mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: vestibulo-cochlear ganglion	Present		2L

Structure	Level	Pattern	Image	Note
TS20: vestibulo-cochlear ganglion	Present	Regionally restricted	2M , 2N	Expression in cochleo-vestibular ganglion neurons

Structure	Level	Pattern	Image	Note
TS20: cochlear duct epithelium	Present	Ubiquitous	2R	Expression in all cells of the cochlear duct.

Structure	Level	Pattern	Image	Note
TS23: cochlear duct	Present	Regionally restricted	3C	Expression in the majority of the cochlear duct cells. Stronger expression in the dorsal portion of the cochlear duct.
TS23: cochlear duct epithelium	Strong	Regionally restricted	3C	Stronger expression especially in the sensory epithelium and in the medial thickened region of the cochlear duct (greater epithelial ridge, GER).
TS23: greater epithelial ridge	Strong	Regionally restricted	3C	Stronger expression especially in the sensory epithelium and in the medial thickened region of the cochlear duct (greater epithelial ridge, GER).
TS23: vestibulo-cochlear ganglion	Present		3C	Authors report expression in the spiral ganglion.

Structure	Level	Pattern	Image	Note
TS23: vestibular component epithelium	Present	Widespread	3F

Structure	Level	Pattern	Image	Note
TS25: cochlear duct epithelium	Present	Regionally restricted	3I	Expression restricted to dorsal portion of cochlear duct (sensory epithelium and GER) and spiral ganglion.
TS25: greater epithelial ridge	Present	Regionally restricted	3I	Expression restricted to dorsal portion of cochlear duct (sensory epithelium and GER) and spiral ganglion.
TS25: vestibulo-cochlear ganglion	Present	Regionally restricted	3I	Expression restricted to dorsal portion of cochlear duct (sensory epithelium and GER) and spiral ganglion.

Structure	Level	Pattern	Image	Note
TS25: organ of Corti	Present	Regionally restricted	3L	Expression in hair cells.
TS25: greater epithelial ridge	Weak	Regionally restricted	3L	Lesser extent of expression in a small proportion of the GER.
TS25: cochlear duct epithelium	Present	Regionally restricted	3L	In the sensory epithelium, no expression in the supporting cell area.

Structure	Level	Pattern	Image	Note
TS25: vestibular component epithelium	Present	Widespread	3O	In the sensory epithelium, no expression in the supporting cell area.

Structure	Level	Pattern	Image	Note
TS23: vestibulo-cochlear ganglion	Present	Regionally restricted	3R	Expression in cochleo-vestibular ganglion neurons

Structure	Level	Pattern	Image	Note
TS25: vestibulo-cochlear ganglion	Present	Regionally restricted	3U	Expression in cochleo-vestibular ganglion neurons

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	4I	Expression in cochlear hair cells in the organ of Corti.
TS27: cochlear ganglion	Present	Regionally restricted	4I	Expression in spiral ganglion neurons.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	4J	Expression in cochlear hair cells in the organ of Corti.

Structure	Level	Pattern	Image	Note
TS27: cochlear ganglion	Present	Regionally restricted	4K	Expression in cochlear hair cells in the organ of Corti.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	4L	Expression in vestibular hair cells.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	4P , 4O	Expression in hair cells.

Structure	Level	Pattern	Image	Note
TS27: cochlear ganglion	Present		4S , 4T	Expression in the spiral ganglion.

Structure	Level	Pattern	Image	Note
TS27: cochlear ganglion	Strong	Regionally restricted		Strong expression specific to hair cells and the spiral ganglion.
TS27: organ of Corti	Strong	Regionally restricted		Strong expression specific to hair cells and the spiral ganglion.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted		Expression in hair cells.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	5C	Expression confined to hair cells.

Structure	Level	Pattern	Image	Note
TS28: cochlear ganglion	Present	Regionally restricted	5F	Expression in spiral ganglion in neuronal cell bodies.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	5I	Espression in hair cells.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	5L	No expression in hair cells. Expression restricted to the inner sulcus.
TS28: osseus spiral lamina	Present	Regionally restricted	5L	Expression in the spiral limbus. Lower expression in interdental cells.

Structure	Level	Pattern	Image	Note
TS28: cochlear ganglion	Present	Regionally restricted	5O	Expression in spiral ganglion in neuronal cell bodies.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	5R	Expression in hair cells.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Absent			No specific staining in organ of Corti or spiral limbus.
TS28: osseus spiral lamina	Absent			No specific staining in organ of Corti or spiral limbus.