Antibody:	Vangl2 antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS11: neural fold	Present		2A

Structure	Level	Pattern	Image	Note
TS11: neural fold	Present		2B	Staining is membrane associated.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Strong		2C	Expression is stronger in the posterior region, where the neural tube is not yet fully closed.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Strong		2D

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		2E
TS17: neural tube lateral wall	Present		2E
TS17: neural tube roof plate	Present		2E

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		2F
TS17: neural tube lateral wall	Present		2F
TS17: neural tube roof plate	Present		2F

Structure	Level	Pattern	Image	Note
TS19: neural tube	Weak		2G , 2H
TS19: neural tube mantle layer	Present		2G , 2H
TS19: neural tube basal columns	Present		2G , 2H	Expression is in the motor columns and in the axons of the commissural neurons as well as in the outgrowing motor and sensory neuronal fibers.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		2I

Structure	Level	Pattern	Image	Note
TS14: foregut epithelium	Weak
TS14: midgut epithelium	Weak

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present
TS17: nephric duct, mesonephric portion	Present
TS17: surface ectoderm	Present
TS17: lung	Present	Regionally restricted		Expression is in the major bronchial ducts.
TS17: forelimb bud	Present	Regionally restricted		Expression is in the most distal part.

Structure	Level	Pattern	Image	Note
TS19: esophagus epithelium	Present
TS19: main bronchus epithelium	Present
TS19: mesonephric tubule	Present
TS19: nephric duct, mesonephric portion	Present
TS19: trachea epithelium	Present

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Present		3A
TS24: cerebral cortex marginal layer	Present		3A
TS24: cerebral cortex mantle layer	Weak		3A
TS24: cerebral cortex ventricular layer	Absent		3A

Structure	Level	Pattern	Image	Note
TS24: basal columns	Strong		3B , 3C

Structure	Level	Pattern	Image	Note
TS24: cranial ganglion	Present		3D
TS24: facial ganglion	Present		3D

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present		3E

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Strong		3G , 3F
TS24: nasal cavity	Moderate	Regionally restricted	3G	Expression is in the ventral (frontal) part of the nasal cavity, in the majority of the cells of the apical and middle layers (but not the basal layer).

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Strong		3H
TS24: nasal cavity	Present	Regionally restricted	3H	Expression in more dorsal and dorso-lateral portions of the nasal cavity, there was membrane-associated expression in fewer cells, predominantly in the apical zone.

Structure	Level	Pattern	Image	Note
TS24: trigeminal ganglion	Present		3I
TS24: cochlea	Strong	Regionally restricted	3I	Expression is in the neurosensory epithelium.
TS24: inner ear	Strong	Regionally restricted	3I	Expression is in the neurosensory epithelium.
TS24: organ of Corti	Very strong		3I

Structure	Level	Pattern	Image	Note
TS24: trigeminal ganglion	Present		3J

Structure	Level	Pattern	Image	Note
TS24: optic nerve	Present		3K
TS24: retina nuclear layer	Present	Regionally restricted	3K	Expression is in the inner nuclear layer.

Structure	Level	Pattern	Image	Note
TS24: organ of Corti	Very strong		3L
TS24: inner ear	Strong	Regionally restricted	3L	Expression is in the neurosensory epithelium.

Structure	Level	Pattern	Image	Note
TS24: cornea	Strong	Regionally restricted	4A	Expression is in the outer layer of the cornea.

Structure	Level	Pattern	Image	Note
TS24: cornea	Strong	Regionally restricted	4B	Expression is in the outer layer of the cornea.
TS24: eyelid epithelium	Strong	Regionally restricted	4B	Expression is in the outer eyelid epithelium.

Structure	Level	Pattern	Image	Note
TS24: gut epithelium	Present		4C

Structure	Level	Pattern	Image	Note
TS24: gut epithelium	Present		4D

Structure	Level	Pattern	Image	Note
TS24: bronchiole epithelium	Present		4E
TS24: lobar bronchus epithelium	Present		4E

Structure	Level	Pattern	Image	Note
TS24: bronchiole epithelium	Present		4F
TS24: lobar bronchus epithelium	Present		4F

Structure	Level	Pattern	Image	Note
TS24: mesenchyme of metanephros	Present		4G
TS24: metanephros	Present		4G
TS24: ureteric trunk	Present		4G

Structure	Level	Pattern	Image	Note
TS24: mesenchyme of metanephros	Present		4H
TS24: metanephros	Present		4H
TS24: ureteric trunk	Present		4H

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present		4I
TS24: hair follicle	Present		4I
TS24: vibrissa follicle	Present		4I

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present		4J
TS24: hair follicle	Present		4J
TS24: vibrissa follicle	Present		4J

Structure	Level	Pattern	Image	Note
TS24: esophagus	Present		4K

Structure	Level	Pattern	Image	Note
TS23: eyelid	Present	Regionally restricted		Expression is at the site of eyelid fusion.

Structure	Level	Pattern	Image	Note
TS24: salivary gland	Present	Regionally restricted		Expression is in epithelia.
TS24: trachea epithelium	Present