Probe:	miR-96 LNA probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS14: otic placode	Absent

Structure	Level	Pattern	Image	Note
TS15: otocyst	Absent		1A

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present		1D	Expression in the entire otic vesicle, cochleo-vestibular ganglion, and all cells of the neural tube.
TS19: inner ear	Present		1D	Expression in the entire otic vesicle, cochleo-vestibular ganglion, and all cells of the neural tube.
TS19: neural tube	Present		1D	Expression in the entire otic vesicle, cochleo-vestibular ganglion, and all cells of the neural tube.

Structure	Level	Pattern	Image	Note
TS20: inner ear vestibular component	Present	Regionally restricted	2A	Expression only in cells of the luminal layer within the vestibular epithelium.

Structure	Level	Pattern	Image	Note
TS20: inner ear vestibular component	Present	Regionally restricted	2D	Expression only in cells of the luminal layer within the vestibular epithelium.

Structure	Level	Pattern	Image	Note
TS22: inner ear vestibular component	Present	Regionally restricted	2G	Expression restricted to hair cells of the luminal layer of the vestibule. No expression in the surrounding mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: vestibulo-cochlear ganglion	Present		2J

Structure	Level	Pattern	Image	Note
TS20: cochlear duct epithelium	Present	Regionally restricted	2P	Expression only in cells of the luminal layer within the cochlear epithelium.

Structure	Level	Pattern	Image	Note
TS23: cochlear duct	Present	Regionally restricted	3A	Expression in the majority of the cochlear duct cells. Stronger expression in the dorsal portion of the cochlear duct.
TS23: cochlear duct epithelium	Strong	Regionally restricted	3A	Stronger expression especially in the sensory epithelium and in the medial thickened region of the cochlear duct (greater epithelial ridge, GER).
TS23: greater epithelial ridge	Strong	Regionally restricted	3A	Stronger expression especially in the sensory epithelium and in the medial thickened region of the cochlear duct (greater epithelial ridge, GER).
TS23: vestibulo-cochlear ganglion	Present		3A	Authors report expression in the spiral ganglion.

Structure	Level	Pattern	Image	Note
TS23: vestibular component epithelium	Present	Widespread	3D

Structure	Level	Pattern	Image	Note
TS25: cochlear duct epithelium	Present	Regionally restricted	3G	Expression restricted to dorsal portion of cochlear duct (sensory epithelium and GER) and spiral ganglion.
TS25: greater epithelial ridge	Present	Regionally restricted	3G	Expression restricted to dorsal portion of cochlear duct (sensory epithelium and GER) and spiral ganglion.
TS25: vestibulo-cochlear ganglion	Present	Regionally restricted	3G	Expression restricted to dorsal portion of cochlear duct (sensory epithelium and GER) and spiral ganglion.

Structure	Level	Pattern	Image	Note
TS25: organ of Corti	Present	Regionally restricted	3J	Expression in hair cells.
TS25: greater epithelial ridge	Weak	Regionally restricted	3J	Lesser extent of expression in a small proportion of the GER.
TS25: cochlear duct epithelium	Present	Regionally restricted	3J	In the sensory epithelium, no expression in the supporting cell area.

Structure	Level	Pattern	Image	Note
TS25: vestibular component epithelium	Present	Widespread	3M	In the sensory epithelium, no expression in the supporting cell area.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	4A	Expression in cochlear hair cells in the organ of Corti.
TS27: cochlear ganglion	Present	Regionally restricted	4A	Expression in spiral ganglion neurons.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	4B	Expression in cochlear hair cells in the organ of Corti.

Structure	Level	Pattern	Image	Note
TS27: cochlear ganglion	Present	Regionally restricted	4C	Expression in cochlear hair cells in the organ of Corti.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	4D	Expression in vestibular hair cells.

Structure	Level	Pattern	Image	Note
TS27: cochlear ganglion	Strong	Regionally restricted		Strong expression specific to hair cells and the spiral ganglion.
TS27: organ of Corti	Strong	Regionally restricted		Strong expression specific to hair cells and the spiral ganglion.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted		No expression in hair cells. Expression in inner sulcus.
TS28: osseus spiral lamina	Present			Expression in the spiral limbus

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	5A	No expression in hair cells. Expression in inner sulcus.
TS28: osseus spiral lamina	Present		5A	Expression in the spiral limbus

Structure	Level	Pattern	Image	Note
TS28: cochlear ganglion	Present	Regionally restricted	5D	Expression in spiral ganglion in neuronal cell bodies.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	5G	No expression in hair cells. Expression in inner sulcus.
TS28: osseus spiral lamina	Present		5G	Expression in the spiral limbus

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	5K	No expression in hair cells. Expression in inner sulcus.
TS28: osseus spiral lamina	Present	Regionally restricted	5K	Expression in the spiral limbus. Lower expression in interdental cells.

Structure	Level	Pattern	Image	Note
TS28: cochlear ganglion	Present	Regionally restricted	5M	Expression in spiral ganglion in neuronal cell bodies.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Absent		5P	No expression in hair cells.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Absent			No specific staining in organ of Corti or spiral limbus.
TS28: osseus spiral lamina	Absent			No specific staining in organ of Corti or spiral limbus.