Probe:	Flrt2 cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: future midbrain neural crest	Present	Regionally restricted	1A	Expression is present in the cranial neural crest, from the forebrain-midbrain border to the midbrain-hindbrain border.
TS13: heart	Present	Regionally restricted	1A	Expression is present in the neural crest cells that contribute to the developing heart primordium.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	1B	Expression is present mesenchyme over the entire midbrain from the dorsal aspect of the midbrain region to the dorsal and posterior aspects of the developing eye.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	1C	Decreasing expression in the midbrain is concomitant with the appearance of expression in the mesenchyme posterior to the maxillary and mandibular primordia.
TS17: 1st branchial arch mandibular component	Present		1C	Expression is present in the mandibular primordia.

Structure	Level	Pattern	Image	Note
TS21: head mesenchyme	Present	Regionally restricted	1D	Expression is confined to the mesenchyme between the eye and the brain vesicle and also ventral and dorsal to the developing ear.
TS21: vibrissa	Present		1D

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme derived from neural crest	Strong		1E	Expression is present in mesenchymal tissues lateral to the neuroepithelium of the midbrain, in migration paths of cranial neural crest cells toward the developing pharyngeal regions.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme derived from neural crest	Strong		1F	Expression is present in mesenchymal tissues lateral to the neuroepithelium of the midbrain, in migration paths of cranial neural crest cells toward the developing pharyngeal regions.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present		1G	Expression is present in the mandibular primordia.
TS17: 1st branchial arch maxillary component	Present		1G	Expression is present in the maxillary primordia.
TS17: head mesenchyme derived from neural crest	Present		1G

Structure	Level	Pattern	Image	Note
TS15: fronto-nasal process ectoderm	Present	Regionally restricted	3A	Expression is present in two stripes on both sides of the developing frontonasal mass in the medial portion of the nasal placodes.
TS15: fronto-nasal process mesenchyme	Present	Regionally restricted	3A	Expression is present in two stripes on both sides of the developing frontonasal mass in the medial portion of the nasal placodes.

Structure	Level	Pattern	Image	Note
TS17: nose	Strong	Regionally restricted	3B	Expression is present in the vomero-nasal organ.
TS17: fronto-nasal process	Strong		3B

Structure	Level	Pattern	Image	Note
TS19: nose	Strong	Regionally restricted	3C	Expression is present in the vomero-nasal organ.
TS19: fronto-nasal process	Strong		3C

Structure	Level	Pattern	Image	Note
TS20: vomeronasal organ	Strong		3D
TS20: fronto-nasal process	Strong		3D

Structure	Level	Pattern	Image	Note
TS15: fronto-nasal process ectoderm	Present	Regionally restricted	3E	Expression is present in two stripes on both sides of the developing frontonasal mass in the medial portion of the nasal placodes.
TS15: fronto-nasal process mesenchyme	Present	Regionally restricted	3E	Expression is present in two stripes on both sides of the developing frontonasal mass in the medial portion of the nasal placodes.

Structure	Level	Pattern	Image	Note
TS17: nose	Present	Regionally restricted	3F	Expression is present in the vomero-nasal organ.
TS17: medial-nasal process mesenchyme	Present		3F

Structure	Level	Pattern	Image	Note
TS19: nose	Present	Regionally restricted	3G	Expression is present in the vomero-nasal organ.
TS19: medial-nasal process mesenchyme	Present		3G

Structure	Level	Pattern	Image	Note
TS20: medial-nasal process mesenchyme	Present		3H

Structure	Level	Pattern	Image	Note
TS19: medial-nasal process mesenchyme	Present		3K

Structure	Level	Pattern	Image	Note
TS22: vomeronasal organ	Present	Regionally restricted	3L	Expression is present on the concave side of the organ.

Structure	Level	Pattern	Image	Note
TS17: oral region	Present	Regionally restricted	3M	Expression is present in the mesenchyme underlying the ectoderm in the putative oral cavity.
TS17: fronto-nasal process	Present		3M

Structure	Level	Pattern	Image	Note
TS21: vomeronasal organ	Present	Regionally restricted	3O	Expression is present on the concave side of the organ.

Structure	Level	Pattern	Image	Note
TS22: vomeronasal organ	Present	Regionally restricted	3P	Expression is present on the concave side of the organ.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf mesenchyme	Present	Regionally restricted	4A	Expression is present in the posterior, but not anterior, of the secondary palatal shelf.
TS21: vomeronasal organ	Present		4A

Structure	Level	Pattern	Image	Note
TS21: palatal shelf mesenchyme	Absent		4B	There is no expression in the anterior portion of the secondary palatal shelf.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf mesenchyme	Present	Regionally restricted	4C	Expression is present in the posterior third of the secondary palatal shelf.

Structure	Level	Pattern	Image	Note
TS23: primary palate mesenchyme	Present	Regionally restricted	4D	Expression is present in the mesenchyme of the posterior part of the palate.

Structure	Level	Pattern	Image	Note
TS23: primary palate mesenchyme	Present	Regionally restricted	4E	Expression is present in the mesenchyme of the posterior part of the palate.

Structure	Level	Pattern	Image	Note
TS23: tongue epithelium	Present		4F

Structure	Level	Pattern	Image	Note
TS22: tooth epithelium	Present		5A	Expression is present in the dental epithelia in the bud to cap stage of tooth development.

Structure	Level	Pattern	Image	Note
TS27: enamel organ	Present	Regionally restricted	5C	Expression is present in Herwig's root sheath.

Structure	Level	Pattern	Image	Note
TS27: enamel organ	Present		5E	Expression is present in Herwig's root sheath. It is also present in the posterior facing aspect of the ameloblastic layer.

Structure	Level	Pattern	Image	Note
TS17: optic cup inner layer	Present		6A

Structure	Level	Pattern	Image	Note
TS23: eyelid epithelium	Present		6C	Expression is present in the epithelial lining of the eye lid.
TS23: lens equatorial epithelium	Present		6C

Structure	Level	Pattern	Image	Note
TS22: vibrissa	Present	Regionally restricted	6E	Expression is present in the outer root sheath.

Structure	Level	Pattern	Image	Note
TS20: vibrissa	Present