Antibody:	HNF4a antibody
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium	Present	Regionally restricted	3A	Expression in most Gcg-expressing cells in the developing pancreas.

Structure	Level	Pattern	Image	Note
TS21: pancreas	Present	Regionally restricted	3C	Relatively strong expression in cells strongly expressing Pdx1.

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present	Regionally restricted	3G , 3E	Expression in some amylase-expressing cells.

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium	Present	Regionally restricted	4A , 4C	Weak expression in a few Isl1-expressing endocrine cells.

Structure	Level	Pattern	Image	Note
TS21: pancreas	Present	Regionally restricted	4D	Strong expression in most Gcg-expressing endocrine cells.

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present	Regionally restricted	4G , 4E	Expression in Amy-expressing acinar cells.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present	Regionally restricted	5C , 5A	Expression in Neurog3-expressing endocrine cell precursors.

Structure	Level	Pattern	Image	Note
TS21: pancreas	Present	Regionally restricted	5F , 5D	Expression in Neurog3-expressing endocrine cell precursors.

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present	Regionally restricted	5I , 5G	Expression in some Neurog3-expressing endocrine cell precursors. Authors report that the frequency of Hnf4a and Neurog3 co-expressing cells decreased as development proceeded.

Structure	Level	Pattern	Image	Note
TS25: pancreas	Present	Regionally restricted	6C , 6A	Expression in islet cells and most acinar cells. Strong expression in a few islet cells.

Structure	Level	Pattern	Image	Note
TS28: pancreatic acinus	Present		6F , 6D	There was expression present in most acinar cells.
TS28: islets of Langerhans	Strong	Regionally restricted	6F , 6D	Strong expression in a few islet cells.

Structure	Level	Pattern	Image	Note
TS28: pancreatic acinus	Present		6I , 6G	There was expression present in the acinar cells.
TS28: islets of Langerhans	Present	Regionally restricted	6I , 6G	Strong expression in a few islet cells.

Structure	Level	Pattern	Image	Note
TS25: pancreas	Present	Regionally restricted	7A	Expression in insulin-expressing beta-cells.

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Present		7C	There was expression present in the beta cells.

Structure	Level	Pattern	Image	Note
TS25: pancreas	Present	Regionally restricted	7E	Expression in insulin-expressing beta-cells.

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Present	Regionally restricted	7G	There was expression present in the alpha cells.

Structure	Level	Pattern	Image	Note
TS25: pancreas	Present	Regionally restricted	7I	Expression in somatostatin-positive gamma-cells.

Structure	Level	Pattern	Image	Note
TS28: endocrine pancreas	Present	Regionally restricted	7K	Expression in somatostatin-positive gamma-cells.

Structure	Level	Pattern	Image	Note
TS25: pancreas	Present	Regionally restricted	7M	Expression in pancreatic polypeptide-expressing cells.

Structure	Level	Pattern	Image	Note
TS28: endocrine pancreas	Present	Regionally restricted	7O	Expression in pancreatic polypeptide-expressing cells.`

Structure	Level	Pattern	Image	Note
TS25: pancreas	Present	Regionally restricted	7Q	Expression in amylase-expressing exocrine cells.

Structure	Level	Pattern	Image	Note
TS28: exocrine pancreas	Present	Regionally restricted	7S	Expression in amylase-expressing cells.