Probe:	Pax2 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	3A'	Expression is present in the intermediate zone of the alar/dorsal plate. It is also present in longitudinal columns at the level anterior to and around the otic vesicle.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	3B'	Expression is present in longitudinal columns in basal/medial plate.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	3G'	Areas of expression include the subventricular zone and the prospective pre-intermediate reticular zone.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	3H'	Expression is present in longitudinal columns. Areas of expression do not include the prepostitus hypoglossal nucleus area.

Structure	Level	Pattern	Image	Note
TS26: medulla oblongata	Present	Regionally restricted		There is moderate expression in the nucleus of solitary tract and dorsal (but not lateral) paragigantocellular nucleus. There is no expression in many nuclei detailed in the table, include the ambiguus, gigantocellular, hypoglossal, and inferior olive.
TS26: pons	Present	Regionally restricted		There is moderate expression in the dorsal cochlear nucleus; none in the ventral. There is weak expression in the prepositus hypoglossal nucleus.
TS26: brainstem	Present	Regionally restricted		There is strong expression in the intermediate reticular zone, parvocellular and rostroventrolateral reticular nuclei, spinal trigeminal nucleus and the intermediate reticular zone. There is moderate expression in the locus coerulus.

Structure	Level	Pattern	Image	Note
TS26: medulla oblongata	Present	Regionally restricted	4B2	Areas of expression include the nucleus of solitary tract.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present		4B5
TS26: medulla oblongata	Present	Regionally restricted	4B5	Areas of expression include the nucleus of solitary tract.
TS26: brainstem	Present	Regionally restricted	4B5	Areas of expression include the spinal trigeminal nucleus.
TS26: pons	Present	Scattered	4B5	There is scattered expression in the prepostitus hypoglossal nucleus.

Structure	Level	Pattern	Image	Note
TS26: pons	Present	Regionally restricted	4B7	Areas of expression include the dorsal cochlear nucleus. It is not expressed in the ventral cochlear nucleus.
TS26: brainstem	Present	Regionally restricted	4B7	Areas of expression include the intermediate reticular zone, the parvocellular reticular nucleus, and the rostroventrolateral reticular nucleus.

Structure	Level	Pattern	Image	Note
TS26: medulla oblongata	Present	Regionally restricted	4C6 , 4C2	Areas of expression include the nucleus of solitary tract.
TS26: brainstem	Present	Regionally restricted	4C6 , 4C2	Areas of expression include the spinal trigeminal nucleus.

Structure	Level	Pattern	Image	Note
TS26: brainstem	Present	Regionally restricted	4C4	Areas of expression include the intermediate reticular zone.
TS26: medulla oblongata	Present	Regionally restricted	4C4	Areas of expression include the dorsal paragigantocellular nucleus. There is no expression in the gigantocellular nucleus.