Probe:	RIKEN clone 2810011G06
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present		1A
TS20: epithalamus	Present		1A
TS20: hypothalamus	Present		1A
TS20: neocortex	Present		1A
TS20: pons	Present		1A
TS20: thalamus	Strong	Regionally restricted	1A	Within the dorsal thalamus, stronger expression in the more ventrocaudal region.
TS20: midbrain	Strong	Regionally restricted	1A	In the superior colliculus, stronger expression in the more caudal region.

Structure	Level	Pattern	Image	Note
TS24: neocortex	Strong	Regionally restricted	1B	Expression level in neopallium was slightly stronger in caudal than in rostral regions.
TS24: cerebral cortex intermediate zone	Moderate		1B
TS24: cerebral cortex subventricular zone	Moderate		1B
TS24: hippocampus	Strong		1B
TS24: telencephalon	Strong	Regionally restricted	1B	Strong expression in the septum.
TS24: tectum	Present	Graded	1B
TS24: inferior colliculus	Strong		1B
TS24: superior colliculus	Moderate	Regionally restricted	1B	Weaker expression in the rostral region of the superior colliculus.
TS24: hypothalamus	Moderate		1B
TS24: main olfactory bulb	Moderate		1B
TS24: pretectum	Moderate		1B
TS24: basal ganglia	Moderate		1B	Authors state that a gradient of expression was not observed.

Structure	Level	Pattern	Image	Note
TS26: neocortex	Present	Regionally restricted	1C
TS26: cortical plate	Present	Regionally restricted	1C	Authors report slightly stronger expression in caudal than in rostral region.
TS26: cerebellum	Present		1C
TS26: hippocampus	Present		1C
TS26: inferior colliculus	Present		1C
TS26: main olfactory bulb	Present		1C
TS26: pons	Present		1C
TS26: telencephalon septum	Present		1C

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Weak		1D	Authors state that expression was almost restricted to the hippocampus, and weaker than those in the embryonic stages.

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present		2A
TS20: neocortex	Present		2A
TS20: thalamus	Present	Regionally restricted	2A	Expression in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present		2B
TS20: neocortex	Present		2B
TS20: thalamus	Present	Regionally restricted	2B	Expression in the dorsal thalamus.
TS20: telencephalon	Present	Regionally restricted	2B	Expression in the hippocampus.

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present		2C
TS20: neocortex	Present		2C
TS20: thalamus	Present	Regionally restricted	2C	Expression in the dorsal thalamus.
TS20: telencephalon	Present	Regionally restricted	2C	Expression in the hippocampus.

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present		2D
TS20: neocortex	Present		2D
TS20: thalamus	Present	Regionally restricted	2D	Expression in the dorsal thalamus.
TS20: telencephalon	Present	Regionally restricted	2D	Expression in the hippocampus.

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Strong		2E
TS22: hippocampus	Strong		2E
TS22: neocortex	Strong	Regionally restricted	2E	Higher expression in dorsomedial than in the lateral region. Stronger expression in caudal regions than in rostral regions.
TS22: thalamus	Present	Regionally restricted	2E	Expression in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Strong		2F
TS22: hippocampus	Strong		2F
TS22: thalamus	Strong	Regionally restricted	2F	High expression in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Strong		2G
TS22: hippocampus	Strong		2G
TS22: neocortex	Strong	Regionally restricted	2G	Higher expression in dorsomedial than in the lateral region. Stronger expression in caudal regions than in rostral regions.
TS22: thalamus	Strong	Regionally restricted	2G	High expression in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Strong		2H
TS22: hippocampus	Strong		2H
TS22: neocortex	Strong	Regionally restricted	2H	Higher expression in dorsomedial than in the lateral region. Stronger expression in caudal regions than in rostral regions.
TS22: thalamus	Strong	Regionally restricted	2H	High expression in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Strong	Regionally restricted	2I	High expression rostrally to caudally with similar expression levels in these regions.
TS24: neocortex	Moderate		2I

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Strong	Regionally restricted	2J	High expressionn rostrally to caudally with similar expression levels in these regions. In caudal regions, expression in the ventricular zone extending to the lining of the most medial portion of the lateral ventricle.
TS24: neocortex	Moderate		2J

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Strong	Regionally restricted	2K	High expression rostrally to caudally with similar expression levels in these regions.
TS24: neocortex	Moderate		2K
TS24: thalamus	Present	Regionally restricted	2K	Expression in differentiating fields of the dorsal thalamus in the rostral region.

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Strong	Regionally restricted	2L	High expression rostrally to caudally with similar expression levels in these regions.
TS24: neocortex	Moderate		2L
TS24: thalamus	Present	Regionally restricted	2L	No expression in the dorsal thalamus in the caudal region.
TS24: epithalamus	Absent		2L

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Strong	Regionally restricted	2M	Strong expression in the hippocampus rostrally to caudally with no detectable expression level gradient in these regions. Moderate expression in the ventricular zone of the hippocampus.
TS26: dentate gyrus	Moderate		2M
TS26: hippocampus CA1	Moderate		2M
TS26: hippocampus CA2	Moderate		2M
TS26: hippocampus CA3	Moderate		2M

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Strong	Regionally restricted	2N	Strong expression in the hippocampus rostrally to caudally with no detectable expression level gradient in these regions. Moderate expression in the ventricular zone of the hippocampus.
TS26: dentate gyrus	Moderate		2N
TS26: hippocampus CA1	Moderate		2N
TS26: hippocampus CA2	Moderate		2N
TS26: hippocampus CA3	Moderate		2N

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Strong	Regionally restricted	2O	Strong expression in the hippocampus rostrally to caudally with no detectable expression level gradient in these regions. Moderate expression in the ventricular zone of the hippocampus.
TS26: dentate gyrus	Moderate		2O
TS26: hippocampus CA1	Moderate		2O
TS26: hippocampus CA2	Moderate		2O
TS26: hippocampus CA3	Moderate		2O

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Strong	Regionally restricted	2P	Strong expression in the hippocampus rostrally to caudally with no detectable expression level gradient in these regions. Moderate expression in the ventricular zone of the hippocampus.
TS26: dentate gyrus	Moderate		2P
TS26: hippocampus CA1	Moderate		2P
TS26: hippocampus CA2	Moderate		2P
TS26: hippocampus CA3	Moderate		2P

Structure	Level	Pattern	Image	Note
TS20: neocortex	Weak	Regionally restricted	3Ae	In the lateral region, weak expression in the preplate, located near the surface of the neopallium.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Strong		3Af	Authors state that signal distribution in the subventricular zone was denser than that in the intermediate zone.
TS22: cerebral cortex subventricular zone	Strong	Regionally restricted	3Af	Within the intermediate zone, stronger expression in deeper region.
TS22: cortical plate	Present	Graded	3Af	Gradient of expression in the cortical plate: strongest in the most superficial region.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Strong	Spotted	3Ag
TS24: cortical plate	Weak		3Ag

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Strong		3Ah
TS26: cerebral cortex intermediate zone	Moderate		3Ah
TS26: cortical plate	Present	Regionally restricted	3Ah	Moderate expression in the most superficial region and deep half of the cortical plate.
TS26: cerebral cortex ventricular zone	Absent		3Ah	No expression in the inner-most zone, corresponding to the ventricular zone.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Strong		3Bb	Expression dramatically increased in cells in the subventricular zone.
TS22: cerebral cortex ventricular layer	Weak		3Bb

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	4E	Expression in differentiating field in the rostral region of the dorsal thalamus.
TS20: neocortex	Present	Regionally restricted	4E	Expression in dorsomedial region stronger than in lateral region; high expression in medial region continued to hippocampus.
TS20: telencephalon	Present	Regionally restricted	4E	Expression in the hippocampus.
TS20: epithalamus	Present	Regionally restricted	4E	Expression in the epithalamus in differentiating fields; no expression in the epithalamic neuroepithelium.

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present	Regionally restricted	4F	Expression in dorsomedial region stronger than in lateral region; high expression in medial region continued to hippocampus.
TS20: telencephalon	Present	Regionally restricted	4F	Expression in the hippocampus.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Moderate	Regionally restricted	4G	Moderate expression in the differentiating fields of basal ganglia.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	4H	Expression in the hypothalamus preferentially detected in the medial region.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong	Regionally restricted	5D
TS22: cortical plate	Moderate	Regionally restricted	5D
TS22: hippocampus	Strong		5D
TS22: thalamus	Strong	Regionally restricted	5D	High expression in the dorsal thalamus.
TS22: epithalamus	Strong		5D

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong		5E
TS22: hippocampus	Strong		5E
TS22: thalamus	Strong	Regionally restricted	5E	High expression in the dorsal thalamus.
TS22: basal ganglia	Moderate	Regionally restricted	5E	Moderate expression in the subventricular zone of the basal ganglia.
TS22: amygdala	Strong	Regionally restricted	5E	In the caudal region, strong expression in part of the amygdaloid nucleus, corresponding to the central nucleus of the amygdala.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong		5F
TS22: cortical plate	Moderate	Regionally restricted	5F	Moderate expression in the superficial region of the cortical plate.
TS22: hippocampus	Strong		5F
TS22: thalamus	Strong	Regionally restricted	5F	High expression in the dorsal thalamus.
TS22: epithalamus	Strong		5F

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Strong		6B
TS22: hippocampus	Strong		6B
TS22: neocortex	Strong		6B
TS22: thalamus	Strong	Regionally restricted	6B	Within the dorsal thalamus, strong expression in the neuroepithelium, with a ventral-high, dorsal-low gradient.
TS22: hypothalamus	Moderate	Regionally restricted	6B	Expression in the medial region of the hypothalamus. Moderate expression in the ventral hypothalamus (except for the inner-most zone, corresponding to the neuroepithelium)

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Strong		6D
TS22: hypothalamus	Present	Regionally restricted	6D	Expression in the medial region of the hypothalamus.

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Present		6F
TS22: hypothalamus	Present	Regionally restricted	6F	Expression in the medial region of the hypothalamus.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Strong		7C
TS22: neocortex	Strong		7C
TS22: thalamus	Strong	Regionally restricted	7C	Within the dorsal thalamus, strong expression in the neuroepithelium, with a ventral-high, dorsal-low gradient.
TS22: diencephalon	Moderate	Regionally restricted	7C	Moderate expression in the subthalamus,

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Strong		7D
TS22: hippocampus	Strong		7D
TS22: thalamus	Strong	Regionally restricted	7D	Within the dorsal thalamus, strong expression in the neuroepithelium, with a ventral-high, dorsal-low gradient.

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Present	Regionally restricted	8B	Expression localized in the CA1 to CA3. In caudal regions, expression in the ventricular zone extending to the lining of the most medial portion of the lateral ventricle.

Structure	Level	Pattern	Image	Note
TS24: epithalamus	Present	Regionally restricted	8D	Expression in differentiating fields of the epithalamus and dorsal thalamus in the rostral region.
TS24: thalamus	Present	Regionally restricted	8D	Expression in differentiating fields of the epithalamus and dorsal thalamus in the rostral region.

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Moderate		9B
TS26: hippocampus CA1	Moderate		9B
TS26: hippocampus CA2	Moderate		9B
TS26: hippocampus CA3	Moderate		9B
TS26: hippocampus	Moderate	Regionally restricted	9B	Expression in the ventricular zone of the hippocampus.
TS26: neocortex	Weak	Regionally restricted	9B	In dorsal region, weak expression around the boundary between the cortical plate and intermediate zone.

Structure	Level	Pattern	Image	Note
TS27: dentate gyrus	Present		10A

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		10B

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		10C

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		10D	Strong expression highly restricted to the dentate gyrus.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present	Uniform	11Ad , 11Ab/c	Expression distributed over the entire thickness of the granule cell layer of the dentate gyrus.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Strong	Regionally restricted	11Ah , 11Af/g	Strong expression in the dentate gyrus only in the deep fraction of the granule cell layer, corresponding to the region where the late-born neurons are located.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present	Regionally restricted	11Bb	Expression in the deep fraction of the granule cell layer.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present	Regionally restricted	11Bd	Expression in the deep fraction of the granule cell layer.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present		S1B

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present	Regionally restricted	S1D	Expression in the cerebellum in the external granular layer and part of the Purkinje layer.