Probe:	capsulin cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: heart atrium	Present	Regionally restricted	3A	Expression in the epicardium of the ventricle and atrium.
TS17: primitive ventricle	Present	Regionally restricted	3A	Expression in the epicardium of the ventricle and atrium.
TS17: hindgut	Present	Regionally restricted	3A	Expression restricted to the mesoderm surrounding the foregut, stomach and hindgut.
TS17: midgut	Present	Regionally restricted	3A	Expression restricted to the mesoderm surrounding the foregut, stomach and hindgut.
TS17: stomach	Present	Regionally restricted	3A	Expression restricted to the mesoderm surrounding the foregut, stomach and hindgut.
TS17: dorsal aorta	Present	Regionally restricted	3A	Expression also prominent in the mesoderm subjacent to the endodermal lining of the coelomic cavity, as well as in the periphery of the dorsal aorta.
TS17: intraembryonic coelom	Present	Regionally restricted	3A	Expression also prominent in the mesoderm subjacent to the endodermal lining of the coelomic cavity, as well as in the periphery of the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS17: visceral pericardium	Present	Regionally restricted	3B	Expression in the epicardium extended over the common atrial and ventricular chambers, and was most dramatic in the atrioventricular sulcus.

Structure	Level	Pattern	Image	Note
TS19: hindgut	Present	Regionally restricted	3C	Expression in the wall of the stomach, midgut and hindgut. Distribution of expression was similar to that described for E10.5.
TS19: midgut	Present	Regionally restricted	3C	Expression in the wall of the stomach, midgut and hindgut. Distribution of expression was similar to that described for E10.5.
TS19: stomach	Present	Regionally restricted	3C	Expression in the wall of the stomach, midgut and hindgut. Distribution of expression was similar to that described for E10.5.
TS19: esophagus mesenchyme	Strong		3C	Prominent expression in the mesoderm surrounding the esophagus and tracheal diverticulum and in the urogenital sinus.
TS19: trachea mesenchyme	Strong		3C	Prominent expression in the mesoderm surrounding the esophagus and tracheal diverticulum and in the urogenital sinus.
TS19: urogenital sinus	Strong		3C	Prominent expression in the mesoderm surrounding the esophagus and tracheal diverticulum and in the urogenital sinus.

Structure	Level	Pattern	Image	Note
TS19: esophagus	Present	Regionally restricted	3D	Expression in the wall of the esophagus and trachea, as well as in a narrow zone surrounding the thymic primordia.
TS19: thymus/parathyroid primordium	Present	Regionally restricted	3D	Expression in the wall of the esophagus and trachea, as well as in a narrow zone surrounding the thymic primordia.
TS19: trachea	Present	Regionally restricted	3D	Expression in the wall of the esophagus and trachea, as well as in a narrow zone surrounding the thymic primordia.

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present		4A
TS20: mesenchyme of metanephros	Present		4A
TS20: midgut mesenchyme	Present		4A
TS20: stomach mesenchyme	Present		4A

Structure	Level	Pattern	Image	Note
TS20: stomach mesentery	Present	Regionally restricted	4B	Mesenchymal expression extends into the gastric omentum.
TS20: gut epithelium	Absent		4B
TS20: lung epithelium	Absent		4B
TS20: mesonephros	Absent		4B

Structure	Level	Pattern	Image	Note
TS20: visceral pericardium	Present		4C	Expression in epicardium
TS20: cardiac muscle tissue	Present	Regionally restricted	4C	Expression in pericardium most prominent in the atrioventricular sulcus, and at the base of the ventricle extending below the epicardial layer into the superficial myocardium.
TS20: pericardium	Present	Regionally restricted	4C	Expression in pericardium most prominent in the atrioventricular sulcus, and at the base of the ventricle extending below the epicardial layer into the superficial myocardium.

Structure	Level	Pattern	Image	Note
TS20: bronchus	Present	Regionally restricted	4D	Expression around the esophagus, primary bronchi and epicardium, especially in the atrioventricular sulcus.
TS20: esophagus	Present	Regionally restricted	4D	Expression around the esophagus, primary bronchi and epicardium, especially in the atrioventricular sulcus.
TS20: heart	Present	Regionally restricted	4D	Expression around the esophagus, primary bronchi and epicardium, especially in the atrioventricular sulcus.

Structure	Level	Pattern	Image	Note
TS20: aortico-pulmonary spiral septum	Present		4E

Structure	Level	Pattern	Image	Note
TS20: gonadal mesenchyme	Present		4F
TS20: hindgut mesenchyme	Present		4F
TS20: mesenchyme of metanephros	Present		4F

Structure	Level	Pattern	Image	Note
TS21: lung	Strong		5A
TS21: urogenital sinus	Strong		5A
TS21: gut mesenchyme	Strong	Regionally restricted	5A	Expression in the gut was distinctly laminated; confined to the submucosal layer which gives rise to the lamina propria. No expression in the peripheral layers of smooth muscle.

Structure	Level	Pattern	Image	Note
TS21: gut mesenchyme	Present	Regionally restricted	5B	Expression restricted to the lamina propria.
TS21: gut epithelium	Absent		5B

Structure	Level	Pattern	Image	Note
TS21: midgut	Present	Regionally restricted	5C	Expression limited to the lamina propria and mesentery.

Structure	Level	Pattern	Image	Note
TS21: esophagus	Strong	Regionally restricted	5D	Prominent expression around the esophagus and in the epicardium.
TS21: visceral pericardium	Strong	Regionally restricted	5D	Prominent expression around the esophagus and in the epicardium.

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of metanephros	Strong		5E
TS21: testis mesenchyme	Strong		5E
TS21: ureteral mesenchyme	Strong		5E
TS21: nephrogenic interstitium	Strong		5E

Structure	Level	Pattern	Image	Note
TS21: hindgut mesenchyme	Strong	Regionally restricted	5F	Prominent expression in the wall of the stomach, in the mesenchyme of the metanephros, the wall of the hindgut, and surrounding the ureters and testes.
TS21: stomach mesenchyme	Strong	Regionally restricted	5F	Prominent expression in the wall of the stomach, in the mesenchyme of the metanephros, the wall of the hindgut, and surrounding the ureters and testes.
TS21: ureteral mesenchyme	Strong	Regionally restricted	5F	Prominent expression in the wall of the stomach, in the mesenchyme of the metanephros, the wall of the hindgut, and surrounding the ureters and testes.

Structure	Level	Pattern	Image	Note
TS28: visceral epithelium of mature renal corpuscle	Present	Regionally restricted	6A	Kidney expression restricted to the podocytes lining the glomerulus.
TS28: renal tubule	Absent		6A	No expression in adjacent tubules.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Regionally restricted	6B	Expression restricted to cells within the interstitium of the heart.

Structure	Level	Pattern	Image	Note
TS28: lung connective tissue	Present	Regionally restricted	6C	Expression in scattered interstitial cells in the alveolar walls in the lung.

Structure	Level	Pattern	Image	Note
TS28: lamina propria of bladder	Present			Expression in urinary bladder submucosa.
TS28: bladder	Present	Regionally restricted		Expression was limited to the interstitial connective tissue. No expression in differentiated smooth muscle layers.
TS28: gut	Present	Regionally restricted		Expression in the lamina propria and submucosa of the gut. Expression was limited to the interstitial connective tissue. No expression in differentiated smooth muscle layers.
TS28: uterus	Present	Regionally restricted		Expression in the stroma of the uterus. Expression was limited to the interstitial connective tissue. No expression in differentiated smooth muscle layers.