Probe:	Klf6 cDNA2
Probe preparation:	Antisense RNA
Assay notes:	The authors state that different RNA probes were generated corresponding to the non-coding or coding regions of the gene; the latter did not contain the highly conserved DNA binding domain. Identical results were obtained with different riboprobes.

Structure	Level	Pattern	Image	Note
TS19: trunk mesenchyme	Present	Regionally restricted	1A/B	Expression is in the mesenchyme surrounding the neural tube.

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present	Regionally restricted	1C/D	Expression is in the mesenchyme surrounding the brain vesicles.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Strong		1E/F , 1H
TS20: hindbrain	Strong		1E/F , 1H
TS20: spinal cord	Strong		1E/F , 1H
TS20: epithelium	Present		1E/F , 1I
TS20: heart	Present		1E/F , 1I
TS20: embryo mesenchyme	Present	Regionally restricted	1E/F , 1H , 1I	Expression is in the loose mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	1J	Expression is in the anteriro hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	1K	At the lumbar and cervical levels expression is in the ventral neural tube.
TS20: basal columns	Present		1K
TS20: trunk mesenchyme	Present	Regionally restricted	1K	Expression is in the mesenchyme surrounding the spinal cord.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	1L	At the lumbar and cervical levels expression is in the ventral neural tube.
TS20: basal columns	Present		1L
TS20: trunk mesenchyme	Present	Regionally restricted	1L	Expression is in the mesenchyme surrounding the spinal cord.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	2A	Expression is in a more dorsal column of cells in the caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	2C	Expression is in a more dorsal column of cells in the caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	2E	Expression is in a more dorsal column of cells in the caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	2G	Expression is in a more dorsal column of cells in the caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	2I	Expression is in a more dorsal column of cells in the caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS21: basal columns	Present	Regionally restricted	3A	Absent at lumbar levels (shown).

Structure	Level	Pattern	Image	Note
TS21: basal columns	Present	Regionally restricted	3C	Expression is also in interneurons in the ventral horns (this section is at the thoracic level).

Structure	Level	Pattern	Image	Note
TS21: basal columns	Present		3E	Shown is cervical level.

Structure	Level	Pattern	Image	Note
TS22: basal columns	Present	Regionally restricted	3G	Expression is restricted to the ventral horns at the forelimb level.

Structure	Level	Pattern	Image	Note
TS22: basal columns	Present	Regionally restricted	3I	Expression is restricted to the ventral horns at the forelimb level.

Structure	Level	Pattern	Image	Note
TS22: basal columns	Present	Regionally restricted	3K	Expression is restricted to the ventral horns at the forelimb level.

Structure	Level	Pattern	Image	Note
TS24: forebrain	Weak		3N/O
TS24: midbrain	Weak		3N/O
TS24: cortical plate	Strong		3N/O
TS24: hypothalamus	Strong		3N/O

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	4D	Expression is in interdigital mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: forelimb interdigital region mesenchyme	Present		4E

Structure	Level	Pattern	Image	Note
TS21: forelimb interdigital region mesenchyme	Present		4F

Structure	Level	Pattern	Image	Note
TS24: gut	Present	Regionally restricted	4G , 4J	Expression is in the mucosa of the hindgut.
TS24: colon epithelium	Present		4J	Absent in the glands.

Structure	Level	Pattern	Image	Note
TS17: extraembryonic component	Strong

Structure	Level	Pattern	Image	Note
TS20: primitive collecting duct	Weak

Structure	Level	Pattern	Image	Note
TS26: gut	Present	Regionally restricted		Expression is in the mucosa of the hindgut.

Structure	Level	Pattern	Image	Note
TS27: hypothalamus	Present
TS27: cerebral cortex	Present	Regionally restricted		Expression is in the cortical plate.
TS27: forebrain	Weak
TS27: midbrain	Weak