Probe:	Tbx12 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: allantois	Strong		3A
TS11: amnion	Present		3A
TS11: cardiogenic plate	Present		3A	Expression is detected in heart precursor cellson either side of the embryonic midline.

Structure	Level	Pattern	Image	Note
TS11: cardiogenic plate	Present		3B	Expression is present in the cardiac crescent at the midline.
TS11: allantois	Strong		3B

Structure	Level	Pattern	Image	Note
TS12: cardiac muscle tissue	Present		3C	Expression levels in the myocardium are higher than those in the endocardium.
TS12: endocardial tube	Present		3C	Expression levels in the myocardium are higher than those in the endocardium.
TS12: allantois	Strong		3C

Structure	Level	Pattern	Image	Note
TS13: heart	Present		3D	Expression is present in most myocardial cells of the looping heart tube.
TS13: lateral plate mesoderm	Present	Regionally restricted	3D	Expression is present in the lateral plate mesoderm immediately below the heart.
TS13: optic pit	Present	Regionally restricted	3D	Expression is present in the dorsal half of the optic vesicle.
TS13: neural tube	Present		3D
TS13: allantois	Strong		3D
TS13: amnion	Absent		3D

Structure	Level	Pattern	Image	Note
TS15: heart	Present		3E	Expression is present in most myocardial cells of the looping heart tube.
TS15: trunk mesenchyme	Present	Regionally restricted	3E	Expression is present in the lateral plate mesoderm immediately below the heart.
TS15: optic cup inner layer	Present		3E	Expression is present in cells that will form the neural retina.
TS15: neural tube	Present		3E

Structure	Level	Pattern	Image	Note
TS15: allantois	Strong

Structure	Level	Pattern	Image	Note
TS17: optic cup inner layer	Present		3F	Expression is present in the neural retina.
TS17: common atrial chamber left part	Present		3F	Expression is present in all chambers of the developing heart.
TS17: common atrial chamber right part	Present		3F	Expression is present in all chambers of the developing heart.
TS17: primitive ventricle	Present		3F	Expression is present in all chambers of the developing heart.
TS17: trunk mesenchyme	Present	Regionally restricted	3F	Expression is present in the lateral plate mesoderm immediately below the heart.

Structure	Level	Pattern	Image	Note
TS17: chorioallantoic placenta	Present			Expression is present in the allantois.

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Present

Structure	Level	Pattern	Image	Note
TS22: left atrium cardiac muscle	Present		3G
TS22: left ventricle cardiac muscle	Present		3G
TS22: right atrium cardiac muscle	Present		3G
TS22: right ventricle cardiac muscle	Present		3G
TS22: outflow tract pulmonary component	Present		3G	Expression is present in the pulmonary trunk.
TS22: outflow tract aortic component	Present		3G	Expression is present in the aortic trunk.

Structure	Level	Pattern	Image	Note
TS27: left atrium	Present		3H	Expression is much stronger in the atria than in the ventricles.
TS27: right atrium	Present		3H	Expression is much stronger in the atria than in the ventricles.
TS27: heart left ventricle	Ambiguous		3H	Expression is much stronger in the atria than in the ventricles.
TS27: heart right ventricle	Ambiguous		3H	Expression is much stronger in the atria than in the ventricles.
TS27: pulmonary trunk	Strong		3H
TS27: aorta	Strong		3H	Expression is present in the aortic trunk.

Structure	Level	Pattern	Image	Note
TS13: cardiac muscle tissue	Present		4A	Expression is much stronger in the myocardium than in the endocardium.
TS13: endocardial tube	Present		4A	Expression is much stronger in the myocardium than in the endocardium.
TS13: lateral plate mesoderm	Present	Regionally restricted	4A	Expression is present in the lateral plate mesoderm immediately below the heart.

Structure	Level	Pattern	Image	Note
TS17: common atrial chamber cardiac muscle	Present		4B	Expression is stronger in the myocardial layer than in the endocardial layer.
TS17: common atrial chamber endocardial lining	Present		4B	Expression is stronger in the myocardial layer than in the endocardial layer.
TS17: primitive ventricle cardiac muscle	Present		4B	Expression is stronger in the myocardial layer than in the endocardial layer.
TS17: primitive ventricle endocardial lining	Present		4B	Expression is stronger in the myocardial layer than in the endocardial layer.
TS17: trunk mesenchyme	Present	Regionally restricted	4B	Expression is present in the lateral plate mesoderm immediately below the heart.

Structure	Level	Pattern	Image	Note
TS22: right atrium cardiac muscle	Present		4C	Expression is stronger in the myocardial layer than in the endocardial layer.
TS22: right ventricle cardiac muscle	Present		4C	Expression is stronger in the myocardial layer than in the endocardial layer.
TS22: right atrium endocardial lining	Ambiguous		4C	Expression is stronger in the myocardial layer than in the endocardial layer.
TS22: right ventricle endocardial lining	Ambiguous		4C	Expression is stronger in the myocardial layer than in the endocardial layer.
TS22: outflow tract aortic component	Strong		4C

Structure	Level	Pattern	Image	Note
TS27: left atrium	Present		4D	Expression is higher in the atria than the ventricles.
TS27: right atrium	Present		4D	Expression is higher in the atria than the ventricles.
TS27: heart left ventricle	Ambiguous		4D	Expression is higher in the atria than the ventricles.
TS27: heart right ventricle	Ambiguous		4D	Expression is higher in the atria than the ventricles.
TS27: aorta	Strong		4D

Structure	Level	Pattern	Image	Note
TS27: heart	Present	Graded		Expression is stronger on the dorsal surface of the heart than on the ventral surface.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Graded		Expression is stronger on the dorsal surface of the heart than on the ventral surface.
TS28: heart atrium	Present			Expression is stronger in the atria than the ventricles.
TS28: heart ventricle	Ambiguous			Expression is higher in the atria than the ventricles.