Probe:	S8 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: somatopleure	Present		4C/D , 4A/B
TS13: somite	Present	Non-Uniform	4C/D , 4A/B	The higher concentration of signal was located ventrally.
TS13: allantois	Present		4A/B
TS13: branchial arch	Present		4A/B
TS13: head mesenchyme	Present		4A/B
TS13: embryo endoderm	Absent		4C/D , 4A/B
TS13: neural tube	Absent		4C/D , 4A/B
TS13: splanchnopleure	Absent		4C/D , 4A/B
TS13: embryo ectoderm	Absent		4A/B , 4C/D	Expression was not found in ectodermal epithelium.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mandibular component	Present		4E/F
TS14: heart	Present		4E/F
TS14: mesenchyme derived from lateral plate	Present		4E/F
TS14: somite	Present		4E/F
TS14: 2nd branchial arch	Present		4E/F	Expression was detected in the hyoid arch.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	5A/B	Expression was concentrated in craniofacial mesenchyme.
TS17: 1st branchial arch mandibular component	Present		5A/B
TS17: 1st branchial arch maxillary component	Present		5A/B
TS17: heart	Present		5A/B
TS17: limb	Present		5A/B
TS17: sclerotome	Present		5A/B

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	5C/D	Expression was detected in a narrow band of ectodermal epithelium in the otic vesicle, adjacent to the positive region in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: fronto-nasal process mesenchyme	Present		6A/B
TS20: nasal septum	Present		6A/B
TS20: palatal shelf	Present		6A/B
TS20: tongue	Present	Regionally restricted	6C , 6A/B	Expression was detected in the mesenchyme. There was no signal in the epithelium.

Structure	Level	Pattern	Image	Note
TS22: face mesenchyme	Present	Regionally restricted	6D	Expression was detected in the mesenchymal layers surrounding the hair follicles.

Structure	Level	Pattern	Image	Note
TS22: lower eyelid mesenchyme	Present	Regionally restricted	7A/B	Expression was detected in the orbital septum and tarsus of the upper and lower eyelids.
TS22: upper eyelid mesenchyme	Present	Regionally restricted	7A/B	Expression was detected in the orbital septum and tarsus of the upper and lower eyelids.
TS22: perioptic mesenchyme	Present	Regionally restricted	7A/B	Expression was detected in the sclera.
TS22: lacrimal gland bud	Present		7A/B

Structure	Level	Pattern	Image	Note
TS22: dental papilla	Present	Regionally restricted	7C/D	Expression was detected in mesenchyme of the dental papilla.

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present	Widespread		Expression was distributed throughout the mesenchyme of the limb bud.

Structure	Level	Pattern	Image	Note
TS20: limb	Present	Regionally restricted	8A/B	Expression was concentrated distally in the limb bud. Hybridization was localized sub-epithelially.
TS20: limb pre-cartilage condensation	Absent		8A/B

Structure	Level	Pattern	Image	Note
TS22: forelimb interdigital region	Present		8C/D
TS22: hindlimb interdigital region	Present		8C/D
TS22: hindlimb mesenchyme	Present	Regionally restricted	8C/D	Expression was detected in connective tissue and distal parts of the limbs.
TS22: forelimb cartilage condensation	Absent		8C/D	There was no signal in the precartilaginous cells.
TS22: hindlimb cartilage condensation	Absent		8C/D	There was no signal in the precartilaginous cells.
TS22: head mesenchyme	Present	Regionally restricted	8C/D	Expression was detected in the head surrounding the whisker follicles.

Structure	Level	Pattern	Image	Note
TS20: sclerotome	Present	Regionally restricted	9A/B	Expression was detected ventro-laterally in the loosely packed mesenchyme of the sclerotome.

Structure	Level	Pattern	Image	Note
TS20: sclerotome	Present		9C/D

Structure	Level	Pattern	Image	Note
TS22: cranium	Present	Regionally restricted	10A/B	Expression was detected in the base of the skull.
TS22: face mesenchyme	Present	Regionally restricted	10A/B	Expression was detected in the fronto-nasal mesenchyme.
TS22: mandible	Present		10A/B
TS22: maxilla	Present		10A/B
TS22: palatal shelf	Present		10A/B
TS22: rib cartilage condensation	Present	Regionally restricted	10A/B	Expression was detected in the perichondrial layers of the ribs.
TS22: genital tubercle	Present	Regionally restricted	10A/B	Expression was detected in the genital eminence.

Structure	Level	Pattern	Image	Note
TS20: heart	Present	Regionally restricted		Expression was mainly restricted to the atrial wall and the atrio-ventricular valves.
TS20: heart atrium	Present	Regionally restricted		Expression was mainly restricted to the atrial wall and the atrio-ventricular valves.