Hoxa13 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:75699 Albrecht AN, et al., The synpolydactyly homolog (spdh) mutation in the mouse -- a defect in patterning and growth of limb cartilage elements. Mech Dev. 2002 Mar;112(1-2):53-67
Assay type:	RNA in situ
MGI Accession ID:	MGI:5446488
Gene symbol:	Hoxa13
Gene name:	homeobox A13

Probe:	Hoxa13 probe7
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Results

Specimen 3 Hoxa13 14.5 wt: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: forelimb digit mesenchyme	Present	Regionally restricted	3_Hoxa13 14.5 wt	Expression in perichondrium and cartilage; No expression in hypertrophic chondrocytes.
TS22: forelimb phalanx cartilage condensation	Present	Regionally restricted	3_Hoxa13 14.5 wt	Expression in perichondrium and cartilage; No expression in hypertrophic chondrocytes.
TS22: forelimb digit 1	Absent		3_Hoxa13 14.5 wt

Specimen 3 Hoxa13 14.5 spdh/spdh: embryonic day 14.5; Hoxd13^spdh/Hoxd13^spdh (more

)

Structure	Level	Pattern	Image	Note
TS22: forelimb digit mesenchyme	Strong	Regionally restricted	3_Hoxa13 14.5 spdh/spdh	Expression in perichondrium and cartilage; No expression in hypertrophic chondrocytes. Authors state that expression was stronger in mutant compared with wildtype.
TS22: forelimb phalanx cartilage condensation	Strong	Regionally restricted	3_Hoxa13 14.5 spdh/spdh	Expression in perichondrium and cartilage; No expression in hypertrophic chondrocytes. Authors state that expression was stronger in mutant compared with wildtype.

Specimen not shown 16.5 wt: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: hand	Present	Regionally restricted	Authors state that expression was comparable to that of Hoxd13.
TS24: forelimb phalanx	Strong	Regionally restricted	Expression more pronounced in distal phalanges
TS24: carpus	Present		Expression in carpal bones.

Specimen not shown 16.5 spdh/spdh: embryonic day 16.5; Hoxd13^spdh/Hoxd13^spdh (more

)

Structure	Level	Pattern	Image	Note
TS24: hand	Present	Regionally restricted		Authors report similar patterns compared with wild type, but expression was generally stronger.

Specimen not shown Newborn wt: postnatal newborn (more

)

Structure	Level	Pattern	Note
TS27: hand bone	Present	Regionally restricted	Authors state that expression was comparable to that of Hoxd13.
TS27: hand connective tissue	Present	Regionally restricted	Authors state that expression was comparable to that of Hoxd13.
TS27: perichondrium	Present	Regionally restricted	Authors state that expression was comparable to that of Hoxd13.
TS27: forelimb distal phalanx	Strong		Expression more pronounced in distal phalanges
TS27: carpal bone	Present

Specimen not shown Newborn spdh/spdh: postnatal newborn; Hoxd13^spdh/Hoxd13^spdh (more

)

Structure	Level	Pattern	Note
TS27: hand bone	Strong	Regionally restricted	Authors report similar patterns compared with wild type, but expression was generally stronger.
TS27: hand connective tissue	Strong	Regionally restricted	Authors report similar patterns compared with wild type, but expression was generally stronger.
TS27: perichondrium	Strong	Regionally restricted	Authors report similar patterns compared with wild type, but expression was generally stronger.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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