Gene Expression Data
Assay Details
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Assay
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Reference:
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J:75699
Albrecht AN, et al., The synpolydactyly homolog (spdh) mutation in the mouse -- a defect in patterning and growth of limb cartilage elements. Mech Dev. 2002 Mar;112(1-2):53-67
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5446473
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Gene symbol:
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Gli1
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Gene name:
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GLI-Kruppel family member GLI1
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Probe:
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Gli1 probe14
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
not shown 10.5 wt:
embryonic day 10.5
(more  )
Specimen
not shown 10.5 wt:
(close  )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
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Expression first observed in posterior margin of limb.
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Specimen
not shown 10.5 spdh/spdh:
(close )
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Genetic Background:
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B6C3Fe a/a-Hoxd13spdh/J
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Mutant Allele(s):
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Hoxd13spdh/Hoxd13spdh
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
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Authors state that the pattern of expression was normal.
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Specimen
2 Gli1 11.5 wt:
embryonic day 11.5
(more )
Specimen
2 Gli1 11.5 wt:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2 Gli1 11.5 spdh/spdh:
(close )
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Genetic Background:
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B6C3Fe a/a-Hoxd13spdh/J
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Mutant Allele(s):
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Hoxd13spdh/Hoxd13spdh
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2 Gli1 12.5 wt:
embryonic day 12.5
(more )
Specimen
2 Gli1 12.5 wt:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2 Gli1 12.5 spdh/spdh:
(close )
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Genetic Background:
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B6C3Fe a/a-Hoxd13spdh/J
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Mutant Allele(s):
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Hoxd13spdh/Hoxd13spdh
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2 Gli1 13.5 wt:
embryonic day 13.5
(more )
Specimen
2 Gli1 13.5 wt:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2 Gli1 13.5 spdh/spdh:
embryonic day 13.5
(more )
Specimen
2 Gli1 13.5 spdh/spdh:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forelimb digit mesenchyme |
Present |
Regionally restricted |
2_Gli1 13.5 spdh/spdh
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Expression is delayed compared with wild type. Continuous expression through phalangeal anlagen, including future joint space, with the exception of the distal interphalangeal joint.
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Analysis Tools