Gene Expression Data
Assay Details
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Assay
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Reference:
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J:75699
Albrecht AN, et al., The synpolydactyly homolog (spdh) mutation in the mouse -- a defect in patterning and growth of limb cartilage elements. Mech Dev. 2002 Mar;112(1-2):53-67
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5446450
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Gene symbol:
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Hoxd13
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Gene name:
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homeobox D13
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Probe:
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Hoxd13 probe17
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2 Hoxd13 10.5 wt:
embryonic day 10.5
(more  )
Specimen
2 Hoxd13 10.5 wt:
(close  )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2 Hoxd13 10.5 spdh/spdh:
(close )
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Genetic Background:
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B6C3Fe a/a-Hoxd13spdh/J
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Mutant Allele(s):
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Hoxd13spdh/Hoxd13spdh
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
2_Hoxd13 10.5 spdh/spdh
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Authors report normal expression domains with respect to anterior and posterior borders, but with reduced overall area of expression compared with wildtype.
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Specimen
2 Hoxd13 11.5 wt:
embryonic day 11.5
(more )
Specimen
2 Hoxd13 11.5 wt:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2 Hoxd13 11.5 spdh/spdh:
(close )
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Genetic Background:
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B6C3Fe a/a-Hoxd13spdh/J
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Mutant Allele(s):
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Hoxd13spdh/Hoxd13spdh
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2 Hoxd13 12.5 wt:
embryonic day 12.5
(more )
Specimen
2 Hoxd13 12.5 wt:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hand |
Present |
Regionally restricted |
2_Hoxd13 12.5 wt/wt
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Expression more restricted to perichondrial region of cartilaginous anlagen.
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Specimen
2 Hoxd13 12.5 spdh/spdh:
(close )
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Genetic Background:
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B6C3Fe a/a-Hoxd13spdh/J
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Mutant Allele(s):
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Hoxd13spdh/Hoxd13spdh
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hand |
Present |
Regionally restricted |
2_Hoxd13 12.5 spdh/spdh
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Reduced expression domain with normal anterior and posterior borders. Expression pattern comparable to that seen at E11.5
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Analysis Tools