Probe:	Cyp26b1 cDNA2
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent		3B
TS10: extraembryonic component	Absent		3B

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Present		3D
TS13: rhombomere 5	Present		3D	Higher level expression in pre-rhombomere 5.

Structure	Level	Pattern	Image	Note
TS14: rhombomere 3	Present		3F
TS14: rhombomere 5	Present		3F
TS14: rhombomere 6	Present		3F
TS14: branchial arch	Present	Regionally restricted	3F	Expressed in the caudal pharyngeal arch region.
TS14: trunk	Present	Regionally restricted	3F , 3F inset	Expressed in the intersomitic regions.
TS14: tail bud	Absent		3F

Structure	Level	Pattern	Image	Note
TS16: forelimb bud	Present		5B
TS16: hindlimb bud	Present		5B
TS16: rhombomere 2	Present		5B
TS16: rhombomere 3	Present		5B
TS16: rhombomere 4	Present		5B
TS16: rhombomere 5	Present		5B
TS16: rhombomere 6	Present		5B
TS16: tail mesenchyme	Present	Regionally restricted	5B	Weak expression in tail bud mesoderm. No expression in the growing tail tip.

Structure	Level	Pattern	Image	Note
TS16: rhombomere 2	Present	Non-Uniform	5D
TS16: rhombomere 4	Present	Non-Uniform	5D
TS16: rhombomere 5	Present		5D	Highest level expression.
TS16: rhombomere 6	Present		5D	Highest level expression.
TS16: rhombomere 3	Present		5D	Weaker expression.

Structure	Level	Pattern	Image	Note
TS16: branchial arch ectoderm	Present		5F	Expressed in defined areas of the caudal branchial arches.
TS16: branchial arch endoderm	Present		5F	Expressed in defined areas of the caudal branchial arches.
TS16: 1st branchial arch maxillary-mandibular groove	Absent		5F	No expression at the maxillo-mandibular cleft.

Structure	Level	Pattern	Image	Note
TS16: 2nd branchial arch ectoderm	Present		5F inset
TS16: 2nd branchial arch endoderm	Present		5F inset

Structure	Level	Pattern	Image	Note
TS18: forelimb bud	Present		5H
TS18: hindlimb bud	Present		5H
TS18: hindbrain	Present		5H
TS18: tail mesenchyme	Weak	Regionally restricted	5H	Weak expression in tail bud mesoderm. No expression in the growing tail tip.

Structure	Level	Pattern	Image	Note
TS16: rhombomere 5	Present	Widespread	6A	Expressed throughout the rhombomere.
TS16: rhombomere 6	Present	Widespread	6A	Expressed throughout the rhombomere.
TS16: rhombomere 2 lateral wall	Present	Regionally restricted	6A	Expressed in the ventral portion.
TS16: rhombomere 3 lateral wall	Present	Regionally restricted	6A	Expressed in the ventral portion.
TS16: rhombomere 4 lateral wall	Present	Regionally restricted	6A	Expressed in the ventral portion. Three longitudinal columns are labeled.
TS16: rhombomere 2 floor plate	Absent		6A
TS16: rhombomere 3 floor plate	Absent		6A
TS16: rhombomere 4 floor plate	Absent		6A

Structure	Level	Pattern	Image	Note
TS18: rhombomere 4	Present	Regionally restricted	6B	Expressed as longitudinal columns.
TS18: rhombomere 5	Present	Regionally restricted	6B	Expressed as longitudinal columns.
TS18: rhombomere 6	Present	Regionally restricted	6B	Expressed as longitudinal columns.
TS18: rhombomere 2	Present		6B
TS18: rhombomere 3	Present		6B

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Regionally restricted	7A	Expressed along the ventral aspect of the bud.
TS15: trunk	Present	Regionally restricted	7A	Expressed in the intersomitic regions.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Regionally restricted	7B	Distally restricted expression at the ventral part.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Non-Uniform	7C	Expressed in both dorsal and ventral locations, and no or very little transcripts in the central, prospective apical ectodermal ridge territory.
TS15: forelimb bud presumptive apical ectodermal ridge	Absent		7C

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Regionally restricted	7D	Distally restricted.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Non-Uniform	7E	Expressed in both dorsal and ventral locations, and no or very little transcripts in the central, prospective apical ectodermal ridge territory.
TS15: forelimb bud presumptive apical ectodermal ridge	Absent		7E

Structure	Level	Pattern	Image	Note
TS15: forelimb bud ectoderm	Present		7F
TS15: forelimb bud mesenchyme	Present	Regionally restricted	7F	Expressed in the superficial mesenchyme.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud ectoderm	Present		7G
TS18: forelimb bud mesenchyme	Present	Regionally restricted	7G	Expressed throughout the distal mesenchyme or progress zone. Highest expression is along the dorsal and ventral regions.

Structure	Level	Pattern	Image	Note
TS19: 2nd branchial arch	Present		8B
TS19: mandibular process	Present		8B
TS19: maxillary process	Present		8B
TS19: midbrain-hindbrain junction	Present		8B
TS19: myelencephalon	Present		8B
TS19: nasal process	Present		8B
TS19: footplate	Strong	Regionally restricted	8B	Expressed along the distal margin.
TS19: handplate	Strong	Regionally restricted	8B	Expressed along the distal margin.

Structure	Level	Pattern	Image	Note
TS19: handplate	Strong	Regionally restricted	8D	Expressed along the distal margin.
TS19: apical ectodermal ridge	Absent		8D

Structure	Level	Pattern	Image	Note
TS19: handplate	Strong	Regionally restricted	8F	Expressed along the distal margin.
TS19: apical ectodermal ridge	Absent		8F

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Absent