Gene Expression Data
Assay Details
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Assay
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Reference:
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J:71419
Reddy S, et al., Characterization of Wnt gene expression in developing and postnatal hair follicles and identification of Wnt5a as a target of Sonic hedgehog in hair follicle morphogenesis. Mech Dev. 2001 Sep;107(1-2):69-82
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5445578
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Gene symbol:
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Wnt10b
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Gene name:
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wingless-type MMTV integration site family, member 10B
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Probe:
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Wnt10b cDNA7
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Probe preparation:
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Antisense labelled with S35 RNA
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Results
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Specimen
3H:
embryonic day 14.5
(more  )
Specimen
3H:
(close  )
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Genetic Background:
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FVB/N
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: epidermis |
Present |
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3H
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Expression was detected in the epidermis. Expression was markedly upregulated in hair follicle placodes.
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Specimen
4A:
embryonic day 14.5
(more )
Specimen
4A:
(close )
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Genetic Background:
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FVB/N
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: epidermis |
Present |
Regionally restricted |
4A
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At the placode stage, expression was specifically upregulated in the placode compared with adjacent interfollicular epidermis.
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Specimen
4D:
embryonic day 18.5
(more )
Specimen
4D:
(close )
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Genetic Background:
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FVB/N
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
4D
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At the hair germ stage, expression was seen in follicular epithelial cells immediately adjacent to the dermal condensate.
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Specimen
4G:
embryonic day 18.5
(more )
Specimen
4G:
(close )
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Genetic Background:
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FVB/N
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
4G
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At the peg stage, expression was seen in follicular epithelial cells immediately adjacent to the dermal condensate.
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Specimen
4J:
embryonic day 18.5
(more )
Specimen
4J:
(close )
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Genetic Background:
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FVB/N
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
4J
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At the bulbous peg stage, expression was localized to a cone of epithelial cells that are inner root sheath precursors.
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| TS26: hair inner root sheath |
Present |
Regionally restricted |
4J
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At the bulbous peg stage, expression was localized to a cone of epithelial cells that are inner root sheath precursors.
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Specimen
5A:
postnatal day 7
(more )
Specimen
5A:
(close )
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Genetic Background:
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BALB/c
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair inner root sheath |
Present |
Regionally restricted |
5A
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Expression was detected in matrix cells that are precursors of the inner root sheath.
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| TS28: hair root sheath matrix |
Present |
Regionally restricted |
5A
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Expression was detected in matrix cells that are precursors of the inner root sheath.
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Specimen
6A:
postnatal day 23
(more )
Specimen
6A:
(close )
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Genetic Background:
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BALB/c
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Age:
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postnatal day 23
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Absent |
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6A
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At telogen stage, expression was not detected in the hair follicle.
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Specimen
6B:
postnatal day 23
(more )
Specimen
6B:
(close )
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Genetic Background:
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BALB/c
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Age:
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postnatal day 23
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
6B
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At anagen stage, expression was detected in follicular epithelial cells immediately overlying the dermal papilla.
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Specimen
6C:
postnatal day 23
(more )
Specimen
6C:
(close )
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Genetic Background:
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BALB/c
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Age:
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postnatal day 23
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
6C
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At anagen stage, expression was detected in follicular epithelial cells immediately overlying the dermal papilla.
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Specimen
7A:
embryonic day 18.5
(more )
Specimen
7A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
7A
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Expression was detected in epithelial cells of germ stage hair follicles.
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Specimen
7B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
7B
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Expression was detected in epithelial cells of germ stage hair follicles as in wild type.
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Analysis Tools