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Gene Expression Data
Assay Details
Assay
Reference: J:52200 Holmes GP, et al., Distinct but overlapping expression patterns of two vertebrate slit homologs implies functional roles in CNS development and organogenesis. Mech Dev. 1998 Dec;79(1-2):57-72
Assay type: RNA in situ
MGI Accession ID: MGI:5445096
Gene symbol: Slit3
Gene name: slit guidance ligand 3
Probe: EST 21651
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 3F: embryonic day 8.5 (more )
Note: ventral view
Structure Level Pattern Image Note
TS13: node Absent 3F
TS13: notochord Absent 3F

Specimen 3G: embryonic day 9.5 (more )
Note: lateral view
Structure Level Pattern Image Note
TS15: branchial groove Present
TS15: future brain roof plate Present 3G There is no expression at the ventral midline region rostral to the rostral midbrain.
TS15: future hindbrain floor plate Present 3G Expression is present in the ventral aspect of the caudal hindbrain.
TS15: neural tube floor plate Present Regionally restricted 3G Expression is present in the rostral spinal cord.
TS15: neural tube lateral wall Present Regionally restricted 3G Expression is present in the rostral spinal cord.
TS15: neural tube roof plate Present 3G
TS15: future hindbrain roof plate Absent 3G There is no expression in the roof plate of rostral hindbrain.
TS15: diencephalon roof plate Absent 3G
TS15: future midbrain roof plate Absent 3G

Specimen 3I: embryonic day 12.0-12.5 (more )
Note: lateral view
Structure Level Pattern Image Note
TS20: spinal cord roof plate Present 3I
TS20: spinal cord floor plate Present 3I
TS20: basal columns Present Regionally restricted 3I Expression is present in the ventral aspect of the spinal cord.
TS20: skin Present Regionally restricted 3I Expression is present extensively over the facial ectoderm. This includes the tissue surrounding the whisker follicles. Expression is found over the presumptive supra-orbital tactile hair follicles.
TS20: vibrissa follicle Absent 3I
TS20: external auditory meatus Present 3I
TS20: medial-nasal process Strong Regionally restricted 3I Expression is present in the medial nasal rim.
TS20: lens vesicle Present 3I
TS20: intestine Present 3I
TS20: somite Present 3I

Specimen 3J: embryonic day 13.5 (more )
Note: lateral view
Structure Level Pattern Image Note
TS21: skin Present Regionally restricted 3J Expression is present in the facial ectoderm. In addition, expression is found over the presumptive infra-orbital tactile hair follicles.
TS21: limb digit Present Regionally restricted 3J Expression is present over the joints.
TS21: external auditory meatus Present 3J
TS21: pinna Present 3J
TS21: nose Present Regionally restricted 3J Expression is present in the medial nasal rim.
TS21: lower lip Present 3J
TS21: tongue Present 3J
TS21: lens Present 3J

Specimen 6E: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present Regionally restricted 6E Expression is present in a diffuse pattern at the tip of the paddle.

Specimen 6F: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hand Present Regionally restricted 6F Expression is present in the anterior and posterior margins, defining the precartilage areas.
TS20: forelimb digit Present Regionally restricted 6F Expression is present at the tip.
TS20: forelimb epithelium Present Diffuse 6F

Specimen 6G: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: forelimb digit Present Regionally restricted 6G Expression surrounds the forming digits, across the presumptive joints, and at the tips of the digits.

Specimen Not shown: embryonic day 10.0 (more )
Note: The authors did not specify which probe was used to obtain these data; it is possible MGI:488634 was used instead.
Structure Level Pattern Image Note
TS16: 1st branchial arch Present
TS16: 2nd branchial arch Present

Specimen Not shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: hindlimb bud mesenchyme Present Regionally restricted Expression is present in a diffuse pattern at the tip of the paddle.

Specimen Not shown: embryonic day 11.5 (more )
Note: The authors did not specify which probe was used to obtain these data; it is possible MGI:488634 was used instead.
Structure Level Pattern Image Note
TS19: genital tubercle Present Regionally restricted Expression is present on both sides.
TS19: stomach Present

Specimen Not shown: embryonic day 13.5 (more )
Note: The authors did not specify which probe was used to obtain these data; it is possible MGI:488634 was used instead.
Structure Level Pattern Image Note
TS21: genital tubercle Present Regionally restricted Expression is present on both sides.
TS21: hindgut Strong Regionally restricted There is strong expression in the diverticulum which may be the developing caecum.
TS21: hindlimb digit Present

Specimen Not shown: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: limb Present Regionally restricted Residual staining is observed over the joints and posterior lateral margins of the limbs.
TS22: limb digit Present Regionally restricted Residual staining is observed over the joints and posterior lateral margins of the limbs.
TS22: skin Trace Regionally restricted Expression in the facial ectoderm is barely detectable.

Specimen Not shown: embryonic day 16.5 (more )
Note: The authors did not specify which probe was used to obtain these data; it is possible MGI:488634 was used instead.
Structure Level Pattern Image Note
TS24: urethra of male Present
TS24: paramesonephric duct Present Expression is present in the Wolffian ducts.

Specimen Not shown: embryonic day 16.5 (more )
Note: The authors did not specify which probe was used to obtain these data; it is possible MGI:488634 was used instead.
Structure Level Pattern Image Note
TS24: urethra of female Present
TS24: paramesonephric duct Present Regionally restricted Expression is present in a ventral line along the paramesonephric ducts of the female.

Specimen Not shown: embryonic day 17.5 (more )
Note: The authors did not specify which probe was used to obtain these data; it is possible MGI:488634 was used instead.
Structure Level Pattern Image Note
TS25: urethra of male Present
TS25: paramesonephric duct Present Expression is present in the Wolffian ducts.

Specimen Not shown: embryonic day 17.5 (more )
Note: The authors did not specify which probe was used to obtain these data; it is possible MGI:488634 was used instead.
Structure Level Pattern Image Note
TS25: urethra of female Present
TS25: paramesonephric duct Present Regionally restricted Expression is present in a ventral line along the paramesonephric ducts of the female.


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last database update
04/30/2024
MGI 6.23
The Jackson Laboratory