Summary | Mutation origin | Mutation description | Recombinase activity | Phenotypes | Find Mice (IMSR) | References

Symbol:	Abcg2tm3.1(cre/ERT2)Bsor
Name:	ATP binding cassette subfamily G member 2 (Junior blood group); targeted mutation 3.1, Brian P Sorrentino
MGI ID:	MGI:5444690
Synonyms:	Abcg2CreERT2, Abcg2-Ires-CreERT2
Gene:	Abcg2  Location: Chr6:58561476-58669436 bp, + strand
Alliance:	Abcg2tm3.1(cre/ERT2)Bsor page

Germline Transmission:	Earliest citation of germline transmission: J:189206
Parent Cell Line:	F12 (ES Cell)
Strain of Origin:	129X1/SvJ

Allele Type:    Targeted (Hypomorph, Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Abcg2tm3.1(cre/ERT2)Bsor expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Abcg2 (MGI:1347061)   Mutation details: A targeting vector was generated with a neomycin resistance cassette, IRES-cre/ERT2 and polyadenylation sequences. The construct was electroporated into ES cells, and homologous recombination inserted the neo-IRES-cre/ERT2-pA sequence into exon 16 of the Abcg2 gene. Correctly targeted ES cells were transiently transfected with a pMC-cre plasmid to excise the nomycin cassette. The ES cells were injected into blastocysts to generate chimeric animals which were used to establish germline transmission. The allele is stated to be hypomorphic for Abcg2 protein expression in the kidney (slight reduction) and liver (greater reduction) determined by Western blot, in homozygous animals. (J:189206)

Activity:	Tissue activity of this recombinase allele
Driver:	Abcg2 (mouse) Summary of all recombinase alleles driven by Abcg2. Alliance mouse Abcg2 gene page

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Abcg2 Mutation:	74 strains or lines available

Original:	J:189206 Fatima S, et al., Abcg2 expression marks tissue-specific stem cells in multiple organs in a mouse progeny tracking model. Stem Cells. 2012 Feb;30(2):210-21
All:	7 reference(s)