Probe:	Dan probe3
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	It was possible that Dan probe4 (MGI:5444517) was also used.

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted	5A	Expression first apparent around E8.5.
TS13: head mesenchyme	Present	Regionally restricted	5A	Expression in head mesenchyme ventral to midbrain.
TS13: head somite	Present	Regionally restricted	5A	Expression in nascent somites, in 2 or more stripes.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	5B	Expression in head mesenchyme encompasses both midbrain and hindbrain regions.
TS15: 1st branchial arch mandibular component mesenchyme	Present		5B
TS15: somite	Present	Regionally restricted	5B	Expression in nascent somite region, now toward caudal end of embryo, and in more mature somites.

Structure	Level	Pattern	Image	Note
TS17: somite	Present		5C	Expression throughout the somite array.
TS17: forelimb bud	Present	Regionally restricted	5C	Expression in dorsoanterior aspect of forelimb bud.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	5D	Expression within head mesenchyme, underlying midbrain and hindbrain.
TS17: branchial arch	Strong	Regionally restricted	5D	Increased expression in branchial region.
TS17: 1st branchial arch mandibular component mesenchyme	Present		5D
TS17: 1st branchial arch maxillary component mesenchyme	Present		5D
TS17: forelimb bud	Strong	Regionally restricted	5D	Increased expression in dorsal aspect of forelimb bud.

Structure	Level	Pattern	Image	Note
TS20: somite	Present		5E
TS20: forelimb mesenchyme	Strong	Regionally restricted	5E	Expression in subepithelial domains. on the dorsal and ventral aspects of forelimb bud.
TS20: hindlimb	Present		5E	Initial expression in hindlimb bud.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	5F	High expresssion in facial primordia.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	6A	Expression predominantly localized to caudal region of most recently formed somite.
TS15: tail unsegmented mesenchyme	Weak	Regionally restricted	6A	Weaker expression in a stripe within presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	6B	Expression within presomitic mesoderm and nascent somites most prominent in a zone adjacent to the neural tube.
TS15: tail unsegmented mesenchyme	Present	Regionally restricted	6B	Expression within presomitic mesoderm and nascent somites most prominent in a zone adjacent to the neural tube.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	6C	In epithelial somites re-initiating expression, expression localized approximately centrally (confined to medial half) within somite boundaries and adjacent to the neural tube.

Structure	Level	Pattern	Image	Note
TS15: sclerotome	Present	Regionally restricted	6D	In older somites, expression predominantly restricted to medial part of sclerotome proximal to neural tube.
TS15: dermomyotome	Absent		6D	No expression in dorsal lip of dermomyotome.

Structure	Level	Pattern	Image	Note
TS20: sclerotome	Present	Regionally restricted	6E	As somites mature, expression becomes progressively restricted. No expression except in caudal-most segments.
TS20: myotome	Present	Regionally restricted	6E	Expression predominantly in developing myotomes, with no apparent restriction along the antero-posterior axis.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	6F	Subepithelial expression in dorsal and ventral domains.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme	Present		6G
TS17: 2nd branchial arch mesenchyme	Present		6G
TS17: 1st branchial pouch endoderm	Present		6G
TS17: 2nd branchial pouch endoderm	Present		6G

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	6H	Expression in cephalic mesenchyme subjacent to midbrain and hindbrain, but not under forebrain.
TS17: branchial arch mesenchyme	Present		6H

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	6I	Subepithelial mesenchymal expression in facial primordia.

Structure	Level	Pattern	Image	Note
TS18: myotome	Weak		7A
TS18: sclerotome	Present		7A	Expression in a striped pattern corresponding to sclerotomes.

Structure	Level	Pattern	Image	Note
TS18: myotome	Weak		7B
TS18: sclerotome	Present		7B

Structure	Level	Pattern	Image	Note
TS18: myotome	Present
TS18: sclerotome	Present	Regionally restricted		Expression in cells adjacent to central portion of neural tube, between maturing dorsal root ganglia.
TS18: vertebral cartilage condensation	Absent			No expression in the vertebral body.

Structure	Level	Pattern	Image	Note
TS20: limb mesenchyme	Present		7C

Structure	Level	Pattern	Image	Note
TS20: limb mesenchyme	Present		7D	No difference in expression pattern compared to wild type embryos.