Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:77274
Stecca B, et al., Gcm1 expression defines three stages of chorio-allantoic interaction during placental development. Mech Dev. 2002 Jul;115(1-2):27-34
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:5444441
|
|
Gene symbol:
|
Gcm1
|
|
Gene name:
|
glial cells missing homolog 1
|
|
Probe:
|
Gcm1 cDNA
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
2A:
(close  )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Gcm1tm1Ral/Gcm1tm1Ral
|
|
Age:
|
embryonic day 7.5-8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: chorion |
Present |
Spotted |
2A
|
Expression is present in a few clusters of 4-8 trophoblast cells at the flat chorionic plate.
|
Specimen
2D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Gcm1tm1Ral/Gcm1tm1Ral
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: chorionic plate |
Strong |
Scattered |
2D
|
Expression is present in small clusters of trophoblast cells in close contact with the allantois; these are separated by a few non-expressing cells.
|
| TS13: trophectoderm |
Strong |
Scattered |
2D
|
Expression is present in small clusters of trophoblast cells in close contact with the allantois; these are separated by a few non-expressing cells.
|
Specimen
6E:
embryonic day 8.75
(more  )
Specimen
6E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.75
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
Specimen
6F:
(close )
Specimen
6G:
embryonic day 10.5
(more )
Specimen
6G:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
Specimen
6H:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: labyrinthine zone |
Present |
|
6D/H
|
Expression levels are dramatically reduced compared to those seen in wild type.
|
|
Analysis Tools