Probe:	Tgfbr2 probe1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS9: trophectoderm	Strong
TS28: decidua	Strong
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS12: allantois	Strong
TS12: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: lung mesenchyme	Present
TS15: lung epithelium	Absent

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present		3A
TS17: body wall mesenchyme	Present		3A
TS17: branchial arch	Present		3A
TS17: branchial pouch	Present		3A
TS17: endolymphatic appendage mesenchyme	Present		3A
TS17: head mesenchyme	Present		3A
TS17: hindgut	Present		3A
TS17: neural tube floor plate	Present		3A
TS17: limb mesenchyme	Present	Regionally restricted	3A	Expression is dorsal and ventral.

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Present		3B
TS20: floor plate	Present		3B
TS20: head mesenchyme	Present		3B
TS20: mandibular process	Present		3B
TS20: maxillary process	Present		3B
TS20: hindlimb mesenchyme	Present	Regionally restricted	3B	Expression is dorsal and ventral.

Structure	Level	Pattern	Image	Note
TS21: choroid plexus	Present		3C
TS21: gut mesenchyme	Present		3C
TS21: head mesenchyme	Present		3C
TS21: lung mesenchyme	Present		3C
TS21: lung parenchyma	Present		3C
TS21: mandible	Present		3C
TS21: maxilla	Present		3C
TS21: meninges	Present		3C
TS21: mesenchyme of metanephros	Present		3C
TS21: pons	Present		3C
TS21: axial skeleton	Present	Regionally restricted	3C	Expression is in intervertebral discs, absent in cartilage.
TS21: metanephros	Present	Regionally restricted	3C	Expression is in basement membrane.
TS21: heart	Present	Regionally restricted	3C	Expression is in endothelial cells.
TS21: liver	Absent		3C
TS21: lung epithelium	Absent		3C
TS21: olfactory epithelium	Absent		3C
TS21: vertebral cartilage condensation	Absent		3C	Absent in the condensing cartilage, and chondrocytes in the rostra1 portion of the vertebral column.

Structure	Level	Pattern	Image	Note
TS23: submandibular gland primordium	Strong		3D
TS23: choroid plexus	Present		3D
TS23: dermis	Present		3D
TS23: gut mesenchyme	Present		3D
TS23: intervertebral disc	Present		3D
TS23: intestine	Present		3D
TS23: limb digit	Present		3D
TS23: lower jaw	Present		3D
TS23: lung parenchyma	Present		3D
TS23: meninges	Present		3D
TS23: palatal shelf	Present		3D
TS23: tongue	Present		3D
TS23: nose	Present	Regionally restricted	3D	Expression is in the mesenchyme surrounding the nasal cavity.
TS23: face	Present	Regionally restricted	3D	Expression is in the whisker pad.
TS23: heart	Present	Regionally restricted	3D	Expression is in endothelial cells.
TS23: epidermis	Absent		3D
TS23: gut epithelium	Absent		3D
TS23: gut muscularis	Absent		3D
TS23: olfactory epithelium	Absent		3D

Structure	Level	Pattern	Image	Note
TS21: axial skeleton	Strong	Regionally restricted	4A	Expression is in the intervertebral discs,
TS21: trunk mesenchyme	Strong	Regionally restricted	4A	Expression is in the loose mesenchyme surrounding the vertebrae.
TS21: cartilage	Absent		4A
TS21: vertebral cartilage condensation	Absent		4A	Absent in the condensing cartilage, and chondrocytes in the rostra1 portion of the vertebral column.

Structure	Level	Pattern	Image	Note
TS23: forelimb digit	Strong	Regionally restricted	4B	Expression is at the tip.
TS23: hand mesenchyme	Strong	Regionally restricted	4B	Expression is in the loose mesenchyme surrounding the periosteum and proliferative cells of the epiphyseal plate.
TS23: cartilage	Absent		4B

Structure	Level	Pattern	Image	Note
TS23: upper jaw mesenchyme	Present	Regionally restricted	4C	Expression is in the mesenchyme cells surrounding the condensing maxillary bone and palate shelf.
TS23: nasopharynx epithelium	Present		4C
TS23: palatal shelf	Present		4C
TS23: cartilage	Absent		4C

Structure	Level	Pattern	Image	Note
TS21: tooth mesenchyme	Present		4D
TS21: tooth epithelium	Absent		4D	Absent in dental lamina.

Structure	Level	Pattern	Image	Note
TS23: dental papilla	Present	Regionally restricted	4E	Expression is restricted to the dental pulp and odontoblast layer.
TS23: incisor mesenchyme	Present	Regionally restricted	4E	Expression is restricted to the dental pulp and odontoblast layer.
TS23: incisor enamel organ	Absent		4E	Absent in ameloblast layer.

Structure	Level	Pattern	Image	Note
TS17: heart	Strong	Regionally restricted	5A	Expression is in endothelial cells.
TS17: endocardial cushion	Strong		5A
TS17: cardiac muscle tissue	Absent		5A

Structure	Level	Pattern	Image	Note
TS22: outflow tract	Strong		5B
TS22: heart	Strong	Regionally restricted	5B	Expression is in endothelial cells.

Structure	Level	Pattern	Image	Note
TS23: mesenchyme of metanephros	Present		5C
TS23: collecting duct	Absent		5C
TS23: pancreas	Absent		5C

Structure	Level	Pattern	Image	Note
TS23: lung parenchyma	Strong		5D
TS23: lung epithelium	Weak	Regionally restricted	5D	Absent in the major bronchiolar epithelial cells. However, expression is in the epithelial lining of the alveoli sac.

Structure	Level	Pattern	Image	Note
TS22: stomach	Strong	Regionally restricted	5E	Expression is in the basement membrane.
TS22: stomach mesenchyme	Strong	Regionally restricted	5E	Expression is in the mesenchyme surrounding the epithelial lining of the stomach.
TS22: duodenum	Strong	Regionally restricted	5E	Expression is in the mesenchyme surrounding the epithelial lining of the duodenum.
TS22: gut epithelium	Absent		5E
TS22: liver	Absent		5E

Structure	Level	Pattern	Image	Note
TS23: intestine	Present	Regionally restricted	5F	Expression is in the stroma of the large intestine villi.

Structure	Level	Pattern	Image	Note
TS23: bladder	Present	Regionally restricted	5G	Expression is restricted to the mesenchymal cells subjacent to the epithelial layer, which has no expression.

Structure	Level	Pattern	Image	Note
TS23: thymus primordium	Present	Uniform	5H

Structure	Level	Pattern	Image	Note
TS21: head mesenchyme	Strong	Regionally restricted	6A	Expression is detected in the mesenchymal cells surrounding the eye.
TS21: eyelid	Present		6A
TS21: cornea	Weak		6A
TS21: lens epithelium	Absent		6A
TS21: lens fiber	Absent		6A
TS21: retina	Absent		6A

Structure	Level	Pattern	Image	Note
TS23: nose	Present	Regionally restricted	6B	Expression is in the mesenchymal cells surrounding the olfactory epithelium.
TS23: olfactory epithelium	Absent		6B

Structure	Level	Pattern	Image	Note
TS23: inner ear mesenchyme	Present	Regionally restricted	6C	Expression is in the mesenchyme surrounding the cochlear duct.
TS23: cochlea epithelium	Absent		6C

Structure	Level	Pattern	Image	Note
TS23: pinna mesenchyme	Present	Regionally restricted	6D	Expression is in mesenchyme adjacent to the condensing cartilage.
TS23: pinna cartilage condensation	Absent		6D

Structure	Level	Pattern	Image	Note
TS23: face	Strong	Regionally restricted	6E	Expression is in the mesenchyme of the whisker pad.
TS23: dermis	Strong		6E
TS23: epidermis	Absent		6E
TS23: vibrissa follicle	Absent		6E

Structure	Level	Pattern	Image	Note
TS23: dermis	Strong	Regionally restricted	6F	Expression is in the dermal papilla as well as in mesenchyme adjacent to the out root sheath of hair follicle.
TS23: epidermis	Absent		6F
TS23: hair follicle	Absent		6F

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component	Present		7A
TS15: 1st branchial arch maxillary component	Present		7A
TS15: heart	Present	Regionally restricted	7A	Expression is in endothelial cells.
TS15: migrating neural crest	Present		7A
TS15: cardiac muscle tissue	Absent		7A
TS15: central nervous system	Absent		7A
TS15: diencephalon	Absent		7A

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present		7B
TS20: hindbrain floor plate	Present	Regionally restricted	7B	Expression is at the midline.
TS20: pituitary gland	Absent		7B

Structure	Level	Pattern	Image	Note
TS21: choroid plexus	Present	Regionally restricted	7C
TS21: head mesenchyme	Present	Regionally restricted	7C
TS21: meninges	Present	Regionally restricted	7C
TS21: pons mantle layer	Present	Regionally restricted	7C
TS21: cerebellum	Absent		7C

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Strong		7D
TS22: pons mantle layer	Strong		7D
TS22: medulla oblongata	Strong	Regionally restricted	7D	Expression is ventral.
TS22: meninges	Present		7D
TS22: hindbrain	Present	Regionally restricted	7D	Expression is at the midline.
TS22: hindbrain roof	Absent		7D
TS22: midbrain	Absent		7D
TS22: midbrain roof plate	Absent		7D

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Present	Regionally restricted	7E
TS20: dorsal root ganglion	Absent		7E
TS20: ganglion	Absent		7E
TS20: notochord	Absent		7E