Probe:	Hox 1.3 probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS12: head	Absent		1b/c
TS12: head somite	Absent		1b/c
TS12: head paraxial mesenchyme	Absent		1b/c	No expression in presomitic mesoderm directly adjacent to head somites.

Structure	Level	Pattern	Image	Note
TS12: trunk unsegmented mesenchyme	Present	Regionally restricted	1e/f	Expression in unsegmented mesoderm in the thoracic region.

Structure	Level	Pattern	Image	Note
TS14: head	Absent		2b/c
TS14: future spinal cord	Present		2b/c
TS14: trunk somite	Present		2b/c

Structure	Level	Pattern	Image	Note
TS15: head	Absent		2d/e
TS15: lung	Present		2d/e
TS15: future spinal cord	Present		2d/e
TS15: trunk somite	Present	Regionally restricted	2d/e	Strong expression in new somites.
TS15: tail	Strong		2d/e	Strong expression in the tail bud region harboring the posterior neuropore.

Structure	Level	Pattern	Image	Note
TS15: trunk paraxial mesenchyme	Present	Regionally restricted	2f/g	Expression in area where somitomeres develop.

Structure	Level	Pattern	Image	Note
TS20: sclerotome	Present	Regionally restricted	3b/c	Expression in sclerotomes 8-17. No expression in sclerotomes 6 and 7.

Structure	Level	Pattern	Image	Note
TS18: trunk sclerotome	Present		3e/f

Structure	Level	Pattern	Image	Note
TS21: rib cartilage condensation	Present	Uniform	4b/c	Expression in rib structures, evenly distributed (homogeneous).
TS21: rib pre-cartilage condensation	Present	Uniform	4b/c	Expression in rib structures, evenly distributed (homogeneous).
TS21: skeletal muscle	Present	Regionally restricted	4b/c	Expression in presumptive trunk muscles, but not in other somite-derived tissues.
TS21: trunk mesenchyme	Present	Regionally restricted	4b/c	Expression in presumptive trunk muscles, but not in other somite-derived tissues.
TS21: limb	Absent		4b/c
TS21: liver	Absent		4b/c

Structure	Level	Pattern	Image	Note
TS26: axial muscle	Absent
TS26: rib	Absent

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present		5b/c
TS20: sclerotome	Present		5b/c
TS20: lung	Strong		5b/c

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Strong		5d/e
TS20: lobar bronchus	Absent		5d/e	No expression in endoderm-derived epithelial cells forming the bronchi
TS20: esophagus	Absent		5d/e

Structure	Level	Pattern	Image	Note
TS20: stomach mesenchyme	Strong		6a/b

Structure	Level	Pattern	Image	Note
TS26: lung	Absent
TS26: stomach	Absent

Structure	Level	Pattern	Image	Note
TS26: gut	Present	Regionally restricted	7b/c	Expression in midgut, more restricted than in E12.0 embryos.

Structure	Level	Pattern	Image	Note
TS26: gut muscularis	Present	Regionally restricted	7d/e	Expression in the longitudinal muscle layer of the gut wall.

Structure	Level	Pattern	Image	Note
TS26: metanephros	Present

Structure	Level	Pattern	Image	Note
TS14: central nervous system	Present	Regionally restricted		No expression in central nervous system ganglia and the brain.
TS14: future brain	Absent			No expression in central nervous system ganglia and the brain.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Regionally restricted	8a/b	Strong expression in posterior region of brain adjacent to the myelencephalon.

Structure	Level	Pattern	Image	Note
TS26: hindbrain	Strong	Regionally restricted	8c/d	Strong expression in posterior region of brain adjacent to the myelencephalon.

Structure	Level	Pattern	Image	Note
TS26: brain	Present	Regionally restricted		Authors report no expression in spinal cord or any other tissue of the central nervous system, including the brain.
TS26: spinal cord	Absent			Authors report no expression in spinal cord or any other tissue of the central nervous system, including the brain.