Antibody:	anti-CRABP I
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	The reference for the antibody describes two antibodies (MGI:1340376 and MGI:4460229); either may have been used in this assay. The fixative was Perfix.

Structure	Level	Pattern	Image	Note
TS11: head mesenchyme	Present	Regionally restricted		In 2 out of 9 cases, expression was detected in a thin stripe of mesenchyme in the region of the future hindbrain, posterior to the head fold.
TS11: embryo endoderm	Absent
TS11: neural ectoderm	Absent

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	3	Expression was detected in the future hindbrain. Two caudal areas of slight convexity were labelled.
TS12: neural ectoderm	Present	Regionally restricted	3	Expression was only detected in the future hindbrain. There was no immunoreactivity in the neural tube rostral or caudal to it.

Structure	Level	Pattern	Image	Note
TS12: neural crest	Present	Regionally restricted	4	Expression was detected in neural crest cells in the mesenchyme around the diencephalon and optic vesicle and as a tongue of cells leaving the most rostral labelled rhombomere.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 4	Present		5
TS13: rhombomere 5	Present		5
TS13: rhombomere	Present	Regionally restricted	5	Expression was detected in rhombomeres 4, 5 and 6.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mesenchyme derived from neural crest	Weak		6
TS14: rhombomere 2	Weak		6
TS14: rhombomere 4	Present		6
TS14: rhombomere 5	Present		6
TS14: rhombomere 6	Present		6
TS14: rhombomere 1	Absent		6
TS14: rhombomere 3	Absent		6
TS14: rhombomere 7	Absent		6	Expression tended to tail off into rhombomere 7.
TS14: 2nd branchial arch mesenchyme derived from neural crest	Strong		6
TS14: 3rd branchial arch mesenchyme derived from neural crest	Strong		6
TS14: future midbrain roof plate	Strong	Single cells	6	Expression extended over the caudal two thirds of the midbrain. Immunoreactivity was not throughout the thickness of the neuroepithelium, but in single neuroblasts on the pial surface of the midbrain.

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Trace		8
TS17: rhombomere 5	Trace		8
TS17: rhombomere 6	Trace		8
TS17: hindbrain mantle layer	Present	Regionally restricted	8	Expression was detected in the axons of the mantle layer throughout the rhombencephalon.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Absent		9
TS13: 2nd branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	9	Expression was observed in a stream of intensely labeled neural crest cells from rhombomere 4, passing rostral to the otic vesicle and into the second branchial arch.
TS13: head mesenchyme derived from neural crest	Present	Regionally restricted	9	Expression was observed in a stream of intensely labeled neural crest cells from rhombomere 4, passing rostral to the otic vesicle and into the second branchial arch.
TS13: 1st branchial arch mesenchyme derived from neural crest	Weak		9
TS13: head mesenchyme	Present	Regionally restricted	9	The mesenchyme surrounding the forebrain is positive.
TS13: head	Present	Regionally restricted	9	Expression was detected in the frontonasal mass.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Absent		10
TS13: head mesenchyme	Present	Regionally restricted	10	The mesenchyme surrounding the forebrain is intensely stained.
TS13: 1st branchial arch mesenchyme derived from neural crest	Weak		10
TS13: 2nd branchial arch mesenchyme derived from neural crest	Present		10
TS13: head mesenchyme derived from neural crest	Present	Regionally restricted	10	The neural crest destined for branchial arch 3 was intensely stained.

Structure	Level	Pattern	Image	Note
TS19: forebrain	Absent		11
TS19: latero-nasal process	Present		11
TS19: medial-nasal process	Present		11