Antibody:	anti-Islet1
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	5A	Expression in cranial nerve III and IV motoneurons
TS15: future hindbrain	Present	Regionally restricted	5A	Authors report expression on either side of the Wnt1 expressing ring (see assay MGI:5441620).
TS15: future midbrain	Present	Regionally restricted	5A	Authors report expression on either side of the Wnt1 expressing ring (see assay MGI:5441620).

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	5B	Authors report expression on either side of the Wnt1 expressing ring (see assay MGI:5441620).

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	5C	Expression in cranial nerve III and IV motoneurons
TS15: future hindbrain	Present	Regionally restricted	5C	Authors report expression on either side of the Wnt1 expressing ring (see assay MGI:5441620).
TS15: future midbrain	Present	Regionally restricted	5C	Authors report expression on either side of the Wnt1 expressing ring (see assay MGI:5441620).

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	5D	Motoneurons of cranial nerve nuclei III and IV are absent. Scattered immunoreactive cells, possibly one or two spared nerve IV motoneurons.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present	Regionally restricted	5E	Expression in cranial nerve III and IV motoneurons
TS17: hindbrain	Present	Regionally restricted	5E	Authors report expression on either side of the Wnt1 expressing ring (see assay MGI:5441620).
TS17: midbrain	Present	Regionally restricted	5E	Authors report expression on either side of the Wnt1 expressing ring (see assay MGI:5441620).
TS17: spinal nerve	Present	Regionally restricted	5E	Expression in spinal motoneurons.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Absent		5F	Motoneurons of cranial nerve nuclei III and IV are absent.
TS17: spinal nerve	Present	Regionally restricted	5F	Expression in spinal motoneurons.

Structure	Level	Pattern	Image	Note
TS19: cranial nerve	Present	Regionally restricted	5G	Expression in motoneurons of cranial nerves III, IV and V.
TS19: midbrain	Present	Regionally restricted	5G	Authors report expression on either side of the Wnt1 expressing ring (see assay MGI:5441620).
TS19: hindbrain	Present	Regionally restricted	5G	Expression in rhombomeres 1 and 2

Structure	Level	Pattern	Image	Note
TS19: cranial nerve	Present	Regionally restricted	5H	Expression in rhombomeres 1 and 2
TS19: hindbrain	Present	Regionally restricted	5H	Expression patterns were truncated to variable extents but not entirely disrupted. No deletions in expression pattern in rhombomere 2.

Structure	Level	Pattern	Image	Note
TS19: cranial nerve	Present	Regionally restricted	5I	Motoneurons of cranial nerve nuclei III and IV are absent.
TS19: hindbrain	Present	Regionally restricted	5I	Expression patterns were truncated to variable extents but not entirely disrupted. No deletions in expression pattern in rhombomere 2.