Probe:	Wnt1 probe20
Probe preparation:	Antisense RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: future brain	Present	Regionally restricted		Authors report no differences in expression between mutant and wild type embryos at E8.5.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	3A	Expression on dorsal and ventral midlines of diencephalon.
TS15: future midbrain	Present	Regionally restricted	3A	Expression on dorsal and ventral midlines of mesencephalon. Expression disappears from scattered cells on the rostral side of the expressing ring.
TS15: future hindbrain	Present	Regionally restricted	3A	Dorsal midline expression interrupted in the metencephalon.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	3B	Expression maintained on the dorsal and ventral midlines of diencephalon and mesencephalon.
TS15: future midbrain	Present	Regionally restricted	3B	Expression maintained on the dorsal and ventral midlines of diencephalon and mesencephalon.
TS15: future hindbrain	Present	Regionally restricted	3B	Dorsal midline expression not interrupted in the metencephalon as it is in the wild type. Scattered patches of ectopically expressing cells in proximity to remnants of the mes/metencephalic ring and near the edge of the cerebellar plate.

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Absent		3C	In mutant embryos where the mes/metencephalic territory was severely shortened, no ring of expression was detected.
TS15: future hindbrain	Present	Regionally restricted	3C	Scattered patches of ectopically expressing cells in proximity to remnants of the mes/metencephalic ring and near the edge of the cerebellar plate. Authors report that expression at the mes/metencephalic junction is often asymmetrical.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	3D	Expression on the dorsal midline.
TS17: midbrain	Present	Regionally restricted	3D	Expression on the dorsal midline.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present	Regionally restricted	3E	In some cases, the mes/metencephalic row of expression cells was interrupted.
TS17: hindbrain	Present	Regionally restricted	3E	Scattered patches of ectopically expressing cells in proximity to remnants of the mes/metencephalic ring and near the edge of the cerebellar plate. Authors describe a short metencephalic extension of the ventral midline expression domain.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present	Regionally restricted	3F	In mutant embryos where the mes/metencephalic territory was severely shortened, the ring of expression was either not detected (left side) or contained clusters of non-expressing cells (right side).
TS17: hindbrain	Present	Regionally restricted	3F	Authors describe a short metencephalic extension of the ventral midline expression domain.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted		Expression maintained on dorsal and ventral midlines of diencephalon and mesencephalon.
TS21: midbrain	Present	Regionally restricted		Expression maintained on dorsal and ventral midlines of diencephalon and mesencephalon.