Gene Expression Data
Assay Details
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Assay
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Reference:
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J:29060
Bally-Cuif L, et al., Involvement of Wnt-1 in the formation of the mes/metencephalic boundary. Mech Dev. 1995 Sep;53(1):23-34
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5441620
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Gene symbol:
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Wnt1
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Gene name:
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wingless-type MMTV integration site family, member 1
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Probe:
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Wnt1 probe20
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 8.0
(more  )
Note: 3-5 somites.
Specimen
2A:
(close  )
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Genetic Background:
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either: C57BL/6J or OF1
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-5 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Punctate |
2A
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Expression in the neural tube with strongly expressing cells juxtaposed with weakly positive or negative cells.
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Specimen
2C1:
embryonic day 8.0
(more )
Note: 6 somites.
Specimen
2C1:
(close )
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Genetic Background:
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either: C57BL/6J or OF1
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Punctate |
2C1
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Expression in the neural tube with strongly expressing cells juxtaposed with weakly positive or negative cells.
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| TS12: future hindbrain |
Present |
Scattered |
2C1
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Scattered groups of cells on the lateral edge of the neural plate up to a patch expressing cells at the level of rhombomere 4.
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Specimen
2C2:
embryonic day 8.5
(more )
Note: 8 somites.
Specimen
2C2:
(close )
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Genetic Background:
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either: C57BL/6J or OF1
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future brain |
Present |
Regionally restricted |
2C2
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Authors describe a ring of expressing cells inside the broader expression domain.
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| TS13: future hindbrain |
Present |
Regionally restricted |
2C2
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Scattered groups of cells on lateral edge of neural plate up to a patch of expressing cells at the level of rhombomere 4.
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Specimen
2C3:
embryonic day 9.0
(more )
Note: 12-14 somites.
Specimen
2C3:
(close )
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Genetic Background:
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either: C57BL/6J or OF1
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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12-14 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future brain |
Present |
Regionally restricted |
2C3
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Expression disappears from scattered cells, first on caudal side of narrowing expressing ring.
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Specimen
2D:
embryonic day 8.0
(more )
Note: 7 somites.
Specimen
2D:
(close )
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Genetic Background:
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either: C57BL/6J or OF1
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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7 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Regionally restricted |
2D
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Expression in the neural tube with strongly expressing cells juxtaposed with weakly positive or negative cells.
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Specimen
2E:
embryonic day 8.5
(more )
Note: 8 somites.
Specimen
2E:
(close )
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Genetic Background:
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either: C57BL/6J or OF1
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future brain |
Present |
Punctate |
2E
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Authors describe a ring of expression.
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Specimen
2F:
embryonic day 9.0
(more )
Specimen
2F:
(close )
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Genetic Background:
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either: C57BL/6J or OF1
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future hindbrain |
Absent |
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2F
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No expression on edge of rhombomere 1 and cerebellar plate.
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Specimen
5A:
embryonic day 9.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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either: C57BL/6J or OF1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5B:
(close )
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Genetic Background:
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involves: B6C3Fe * C57BL/6J
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Mutant Allele(s):
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Wnt1sw/Wnt1sw
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5C:
embryonic day 9.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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either: C57BL/6J or OF1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5D:
(close )
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Genetic Background:
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involves: B6C3Fe * C57BL/6J
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Mutant Allele(s):
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Wnt1sw/Wnt1sw
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5E:
embryonic day 10.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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either: C57BL/6J or OF1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5F:
(close )
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Genetic Background:
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involves: B6C3Fe * C57BL/6J
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Mutant Allele(s):
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Wnt1sw/Wnt1sw
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools