Gene Expression Data
Assay Details
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Assay
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Reference:
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J:29060
Bally-Cuif L, et al., Involvement of Wnt-1 in the formation of the mes/metencephalic boundary. Mech Dev. 1995 Sep;53(1):23-34
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5441618
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Probe:
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Otx2 probe23
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Probe preparation:
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Antisense RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
not shown:
embryonic day 8.0; Wnt1sw/ Wnt1sw
(more  )
Note: 1-9 somites.
Specimen
not shown:
(close  )
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Genetic Background:
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involves: B6C3Fe * C57BL/6J
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Mutant Allele(s):
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Wnt1sw/Wnt1sw
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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1-9 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Regionally restricted |
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Expression indistinguishable from that of wild type or heterozygous littermates, in particular on the presumptive dorsal midline.
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Specimen
not shown:
embryonic day 8.5; Wnt1sw/ Wnt1sw
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: B6C3Fe * C57BL/6J
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Mutant Allele(s):
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Wnt1sw/Wnt1sw
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future brain |
Present |
Regionally restricted |
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Expression indistinguishable from that of wild type or heterozygous littermates, in particular on the presumptive dorsal midline.
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Specimen
3G:
(close )
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Genetic Background:
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involves: B6C3Fe * C57BL/6J
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Mutant Allele(s):
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Wnt1sw/Wnt1sw
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future brain |
Present |
Regionally restricted |
3G
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Authors state that the caudal limit of expression was perturbed.
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| TS15: future hindbrain |
Present |
Regionally restricted |
3G
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Ectopic patches of expressing cells, more abundant near the presumptive mes/metencephalic limit and the edge of the cerebellar plate.
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Specimen
3H:
embryonic day 10.5
(more )
Specimen
3H:
(close )
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Genetic Background:
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either: C57BL/6J or OF1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3I:
(close )
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Genetic Background:
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involves: B6C3Fe * C57BL/6J
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Mutant Allele(s):
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Wnt1sw/Wnt1sw
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: brain |
Present |
Regionally restricted |
3I
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Authors state that the caudal limit of expression was perturbed.
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| TS17: rhombomere 1 |
Present |
Regionally restricted |
3I
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Ectopic patches of expressing cells, spreading into rhombomere 1, but not crossing the boundary between rhombomeres 1 and 2.
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Specimen
not shown:
embryonic day 14.5; Wnt1sw/ Wnt1sw
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: B6C3Fe * C57BL/6J
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Mutant Allele(s):
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Wnt1sw/Wnt1sw
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brain |
Present |
Regionally restricted |
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Caudal limit of expression was perturbed.
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Specimen
not shown:
embryonic day 15.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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either: C57BL/6J or OF1
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: brain |
Present |
Regionally restricted |
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Authors report sharp caudal limit of expression until at least E15.5.
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Analysis Tools
